• Patchliner

    兼顾科研与工业的全自动膜片钳系统
  • Patchliner

    自主生产与质量控制的耗材
  • Patchliner

    拥有超过10年实验设计与支持的经验
  • Dynamite8

    Automated Dynamic Clamp
  • Patchliner

    拥有所有手动膜片钳的特征与优点

NaV1.8 - "Characterization of rNaV1.8 (ND7-23) on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.4 MB)
Cells were kindly provided by Millipore.

Summary:

The NaV1.8 gene (originally named PN3 or SNS; gene symbol SCN10A) encodes a voltage-gated sodium (NaV) channel, selectively expressed in dorsal root ganglion (DRG) neurons. DRGs transmit peripheral stimuli to the central nervous system and are involved in nociception. Different NaV channels play a key role in modulation of DRG action potentials. In particular, the fast upstroke of the action potential is mediated by NaV channels. NaV channels are in part characterized by their TTX-sensitivity (TTX-resistant [TTXr], TTX-sensitive [TTXs]). NaV1.8 is a TTXr channel. Compared with other NaV channels, NaV1.8 has slow activation and inactivation kinetics and is opened at relatively high  voltages. It is an interesting drug target for inflammatory and neuropathic pain, because modulation of NaV1.8 by inflammatory mediators seems to be a key mechanism of DRG nociceptor sensitization and activation. Interestingly, NaV1.8 has been reported to play an important role in the perception of cold pain. In this Application Note we present data recorded on the Patchliner characterizing ND7-23 cells (a rat DRG/mouse neuroblastoma hybrid) stably transfected with rat NaV1.8. All experiments were performed in the presence of 100 nM TTX to block the endogenous TTXs Na+ current present in these cells. The NaV1.8 activation and inactivation properties and tetracaine sensitivity recorded on the Patchliner were consistent with those reported in the literature.

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