Cardiomyocytes - "Recordings of action potentials in mouse ES cell-derived Cor.At cardiomyocytes on Nanion's Patchliner"
Patchliner application note: (0.7 MB)
Cells were kindly provided by Axiogenesis.
Cor.At® cardiomyocytes display typical cardiac ion channel activity and action potentials. Both the hERG blockers Quinidine and Cisapride, and the Na+-channel blocker Lidocaine, modulated the action potentials. The Quinidine and Lidocaine effects were reversible, the Cisapride effect was non-reversible. The results demonstrate the presence of an array of ion channels in Cor.At® cardiomyocytes which in conjunction are capable of generating action potentials. Hence these stem cell-derived cardiomyocytes are a suitable alternative to primary cardiomyocytes in drug screening and safety testing. In addition, these experiments demonstrate for the first time the suitability of a higher throughput planar patch clamp system, i.e. Nanion´s Patchliner®, for recording action potentials. This is possible because of the flexibility of Nanion‘s patch clamp systems allowing for a multitude of different experiments to be performed in both voltage and current clamp modes. Cells expressing multiple ion channels and therefore able to elicit action potentials, as opposed to cell lines overexpressing a single ion channel subtype, better represent the physiological system. Thus, the Patchliner® in combination with Cor.At® cardiomyocytes form a powerful tool for ion channel research, drug screening and safety testing.