2022 - Using single-chain variable fragments (scFv) to map the β3-subunit binding site on the pain-sensing sodium channel Nav1.7
Patchliner Poster Cambridge Ion Channel Forum 2022 (7.04 MB)
β3-subunit is co-expressed with Nav1.7 in painsensing neurons, but its binding site remains unknown. We used in silico modelling to predict the β3 binding site. We used scFvs against Nav1.7 domain I extracellular loop (L1) and domain IV extracellular loop (L4) to test this model.