Port-a-Patch mini -更小巧而强大的全自动膜片钳

随着Port-a-Patch mini的发布，世界上最小的膜片钳装置就变得更小巧了。基于Port-a-Patch的成功，我们通过将放大器集成到工作站中，从而进一步缩小了膜片钳系统。 Port-a-Patch mini一次支持对一个细胞进行千兆封接记录，只需简短的培训即可快速轻松地获得高质量的膜片钳数据。它不仅是功能强大的研究工具，还是用于教学目的以及细胞和离子通道快速测试的理想之选。 Port-a-Patch mini的优点包括：

小型且价格合理的完整膜片钳系统
尽量减少测试细胞和待测物的花费
内置放大器
不需要膜片钳经验
适合教学
支持电压，电流钳
支持全细胞，穿孔膜记录
Nanion自产芯片，提供多种电阻规格 (可另外定制芯片规格)
手动，无限制加入待测物
高成功率（细胞系），也可成功用于原代细胞与干细胞
易于使用，只需要简单培训

使用Port-a-Patch mini既简单又容易-用户只需将溶液和细胞添加到一次性记录芯片上，即可使用计算机控制的气泵通过抽吸自动捕获和封接细胞。

Port-a-Patch微型系统由带内置放大器的Port-a-Patch微型记录工作站，抽吸控制器和计算机组成。

更多信息:

PatchControl mini

PatchContol mini: the intuitive Software of the Port-a-Patch mini

The PatchControl mini is a software based on the well-known PatchControl software of the Port-a-Patch and optimized for the Port-a-Patch mini allowing the easiest way to perform your sealing procedure. Equipped with a set of pre-written protocols fitting most user scenarios to obtain a fully automated procedure to rech whole cell configuration, the protocols can also be altered, allowing the entire procedure for cell capture, sealing and obtaining the whole cell configuration to be completely customizable. However, because we know that sometimes you just want to control things yourself, the protocols can be interrupted at any time and the suction controlled manually.

Through innovative communication with the electrophysiology software WinWCP, PatchControl mini can read out important parameters like membrane resistance, whole cell capacitance and series resistance and uses those to perform the pre-programmed automated steps accordingly. Suction strength and duration, voltage and membrane criteria can be set and altered to obtain the optimum parameters for cells routinely used in your lab.

The PatchControl mini software is there to make your patch clamp life easier, from automated cell capture to automated pressure control, the PatchControl mini software can do it all!

耗材

NPC-1芯片

Products NPC1 330

The NPC-1 chip is a proprietary innovative product by Nanion Technologies, developed for the Port-a-Patch. It is produced and quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-1 chips are available which should be chosen depending on cell size and application.

材料

The core structure of the NPC-1 chip is a planar borosilicate glass slide with its micron-sized patch aperture. This structure is incorporated into a screw cap for easy handling and minimal cycle times between experiments. Additionally, the unique design of the NPC-1 chip gives similar access to the external and internal solution. Continuous perfusion of the internal solution has finally become possible.

可提供的芯片类型

"NPC-1, 1-2 MOhm": (Order # 061101)
"NPC-1, 2-3.5 MOhm": (Order # 061102)
"NPC-1, 3-5 MOhm": (Order # 061103)
"NPC-1, 5-10 MOhm": (Order # 061104)
"NPC-1, 8-12 MOhm": (Order # 061105)
"NPC-1, 10-15 MOhm": (Order # 061106)

其他孔径也可以定制!

缓冲液与溶液

Buffers and Solutions for the Port-a-Patch mini

Reliable buffer solutions are critical for any electrophysiological application. Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability. Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. Our buffers are shipped with the corresponding "Certificates of Analysis" and "Material Safety Data Sheets" (MSDS).

Available buffers and solutions

"External Standard", 500 mL: (Order # 08 3001)
"External Standard Ca 10", 500 mL: (Order # 08 3012)
"External NMDG 60", 500 mL: (Order # 08 3004)
"External NMDG 60 Ca 10", 500 mL: (Order # 08 3011)
"Internal CsF 110", 500 mL: (Order # 08 3008)
"Internal KF 110", 500 mL: (Order # 08 3007)
"Reagent Kit Port-a-Patch Standard", Cryo box with 81 vials, incuding 27x External standard, 18x External Ca 35, 18x Internal KF 60, 18x Internal CsF 60 (Order # 08 1001)

数据与应用

Astrocytes - Current Traces of Potassium Channels

Port-a-Patch mini data and applications:
Native mouse Astrocytes.

Patch clamp recordings from cultured mouse primary astrocytes were made in the whole cell configuration. Currents of expressed Potassium channels were obtained by depolarizing voltage pulses from a holding potential of −80 mV.

Chondrocytes - Current response to voltage ramp

Port-a-Patch mini data and applications:
Native Bovine Chondrocytes were kindly provided by Dr. Rebecca Lewis, University of Surrey.

Patch clamp recordings from primary chondrocytes were made in the whole cell configuration. The raw current trace was obtained by using a ramp protocol starting from -100 mV to 100 mV.

hERG - Application of Dofetilide

Port-a-Patch mini data and applications:
Cells were kindly provided by SB Drug Discovery.

Raw data traces of hERG (expressed in HEK cells) in control solution and in the presence of increasing concentrations of Dofetilide (top). IC₅₀ concentration response curve for an average of 3 cells is shown revealing an IC₅₀ of 161 ± 12 nM.

NaV1.5 - Raw current traces and Current-Voltage Relationship

Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.

The image shows currents from an example cell and the corresponding current-voltage plot for Na_V1.5 expressed in stably-transfected CHO-K1 cells.

NaV1.5 - Tetracaine concentration response curve

Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.

The image shows the timecourse of the experiment (top) where peak amplitude is plotted against time and example traces in control conditions and different concentrations of tetracaine are also shown in the insert. The concentration reponse curve for tetracaine is shown on the bottom for an average of 6 cells. The curve is fit with a Hill equation revealing an IC₅₀ = 25.5 ± 5.7 µM (n = 6).

NaV1.5-Late - Recorded from Cardiosight-S hiPSC-CMs

Port-a-Patch mini data and applications:
Cells were kindly provided by Nexel.

NaV-Late was induced by application of ATX II in Cardiosight-S^® cells on the Port-a-Patch mini. Example traces are shown on the top with the response to the voltage ramp (CiPA protocol) shown in the inset. The timecourse of the experiment is shown on the bottom, with block by ranolazine.