Port-a-Patch - 世界上最小的膜片钳系统
Port-a-Patch是一台支持单细胞千兆欧姆级封接小型化膜片钳系统,只需很少时间的培训即可快速、轻松地获得高质量的膜片钳数据。因此,Port-a-Patch不仅是一款强大的科研工具、也是一款用于教学和快速检测细胞与离子通道的理想工具。
Port-a-Patch有以下一些特点:
- 外液和内液灌流功能
- 支持温度控制
- 支持电流钳和电压钳
- 支持全细胞记录与穿孔膜片钳记录
- 严格质控、自主生产的单孔与多孔芯片
- 支持串联电阻Rs补偿
- 支持无限次灌流
- 电压与配体门控离子通道研究
- 可记录细胞系、原代细胞和干细胞
- 易学易用
- 可在实验过程中随时调整参数,实现快速高效的实验开发
Port-a-Patch的操作简单、直接——用户只需要在芯片上加入溶液与细胞,通过负压控制器即可自动捕获并封接细胞,而不需要显微镜与微操。
Port-a-Patch的内外灌流系统、温度控制系统等配件以及支持电流钳和脂双层实验的功能极大地扩展了科学研究的实验范围。
Port-a-Patch系统由Port-a-Patch工作站、负压控制器、HEKA放大器和电脑组成。如果您已有放大器,大部分情况下放大器是可以直接整合到Port-a-Patch系统中。 另外,还有一些可用于特殊实验的附件:温度控制附件、显微成像附件、自动灌流系统等。
更多信息:
附件与功能
温度控制与外灌流系统
Port-a-Patch的外灌流系统由计算机控制的磁性夹管阀面板和专用的温控流动室(TC Flow Chamber)组成。 灌流面板最多可处理八种不同的溶液。溶液既可以手动切换,也可以通过软件切换。 后者允许数据采集与溶液交换同步,以及标注所应用的化合物和浓度等相关信息。
配备灌流板的温控灌流室的溶液交换时间<150 ms,非常适合研究配体和电压门控通道。温控灌流室的温度控制功能允许在高温和低温下进行实验或应用短时间的温度脉冲。 内含两个部件用于加热或冷却溶液:用于在连续灌注期间冷却/加热溶液的套管,以及集成在屏蔽盖中的Peltier元件。 无论细胞是否灌注,都可以保持稳定的温度。 温度传感器将提供浴液的实时信息,并在PatchControl中进行监控,只需点击一下按钮即可调节温度。
内灌流系统
Nanion的平面膜片钳芯片提供了灌注膜细胞内侧的独特可能性。 这个功能被用于Port-a-Patch的内液灌流系统。 在细胞内可以灌流多达八种不同的溶液,允许对作用于离子通道的胞质部分的化合物进行剂量反应分析。 还可以在内部添加第二信使和代谢物以研究离子通道功能如何受到影响。 该系统可以在几秒钟内交换细胞内溶液,并且在溶液交换期间可以连续监测离子通道信号。
内灌流系统是Port-a-Patch的附加组件。 结合Nanion的灌流系统,它可以手动操作,也可以完全由计算机控制。 通过这种方式,数据采集可以与溶液交换同步,并且自动标记化合物信息。
Port-a-Patch 显微镜载玻片
Nanion提供Port-a-Patch显微镜载玻片,用于同步进行细胞可视化记录和膜片钳记录。 可以将直立显微镜的荧光测量结果与当前膜片钳记录组合。
负压控制器 Pro
负压控制器Pro包含一个用于与膜片钳放大器的模拟输入和输出通信的接口。 通过这种通信途径,施加压力可以与电压protocol同步,并实现同时记录。
负压控制器Pro非常适合研究机械敏感离子通道和研究压力对人工膜与细胞膜的影响。 该附加组件可与Port-a-Patch一起使用。 它与所有现有的Port-a-Patch系统兼容。
The Port-a-Patch SOL
The Port-a-Patch SOL enables optical stimulation during recordings on the Port-a-Patch by covering it with a dedicated lid to which up to three light sources can be connected.
The Port-a-Patch SOL is available as a kit or as an Add-On.
The Port-a-Patch SOL Kit consists of an optical lid and one high-power LED light source. The light source can either controlled manually or by the amplifier software.
The Add-On does not include a light source, so you are free to use light sources you already have.
PatchControl软件
PatchContol: Port-a-Patch的简单易用的软件
PatchControl是为用户定制的软件。可以加载和更改预先编写的protocol,从而使得细胞捕获,密封和获得全细胞的整个配置的过程完全自动化。同时,我们知道有时你也想自己控制实验,所以也可以随时中断protocol并手动控制。 p>
通过与电生理学软件的数据交换,PatchControl可以读取膜电阻,全细胞电容和串联电阻等重要参数,并相应地执行预编程步骤。可以设置和更改气压强度和持续时间,电压和膜电容电阻等参数,以获得实验室常规使用的细胞的最佳参数。不仅如此,复杂的宏可以轻松实现从简单的增益变化到复杂的灌流程序,编程和自动执行。 p>
PatchControl软件可以让您的贴片钳实验更轻松,从自动细胞捕获到自动压力控制,PatchControl软件可以做到这一切!
耗材
NPC-1芯片
The NPC-1 chip is a proprietary innovative product by Nanion Technologies, developed for the Port-a-Patch. It is produced and quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-1 chips are available which should be chosen depending on cell size and application.
材料
The core structure of the NPC-1 chip is a planar borosilicate glass slide with its micron-sized patch aperture. This structure is incorporated into a screw cap for easy handling and minimal cycle times between experiments. Additionally, the unique design of the NPC-1 chip gives similar access to the external and internal solution. Continuous perfusion of the internal solution has finally become possible.可提供的芯片类型
- "NPC-1, 1-2 MOhm": (Order # 061101)
- "NPC-1, 2-3.5 MOhm": (Order # 061102)
- "NPC-1, 3-5 MOhm": (Order # 061103)
- "NPC-1, 5-10 MOhm": (Order # 061104)
- "NPC-1, 8-12 MOhm": (Order # 061105)
- "NPC-1, 10-15 MOhm": (Order # 061106)
其他孔径也可以定制!
缓冲液与溶液
Buffers and Solutions for the Port-a-Patch
Reliable buffer solutions are critical for any electrophysiological application. Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability. Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. Our buffers are shipped with the corresponding "Certificates of Analysis" and "Material Safety Data Sheets" (MSDS).
Available buffers and solutions
- "External Standard", 500 mL: (Order # 08 3001)
- "External Standard Ca 10", 500 mL: (Order # 08 3012)
- "External NMDG 60", 500 mL: (Order # 08 3004)
- "External NMDG 60 Ca 10", 500 mL: (Order # 08 3011)
- "Internal CsF 110", 500 mL: (Order # 08 3008)
- "Internal KF 110", 500 mL: (Order # 08 3007)
- "Reagent Kit Port-a-Patch Standard", Cryo box with 81 vials, incuding 27x External standard, 18x External Ca 35, 18x Internal KF 60, 18x Internal CsF 60 (Order # 08 1001)
Buffer Solutions - Product Sheet
SyncroPatch 384,
Patchliner and
Port-a-Patch - Quality-assured buffer solution product sheet:
(2.8 MB)
数据与应用
AMPA Receptor (GluA2) - Reproducible Responses
Port-a-Patch data and applications:
Cells were kindly provided by SB Drug Discovery.
GluA2 reproducibly recorded on the Port-a-Patch. L-glutamate was applied for 500 ms and this was repeated six times in the same cell showing reproducible responses.
AMPA Receptor (GluA2) - Fast Perfusion with the Port-a-Patch
Port-a-Patch data and applications:
Cells were kindly provided by SB Drug Discovery.
The AMPA receptor (GluA2) was activated by increasing concentrations of glutamate on the Port-a-Patch. L-glutamate was applied for approximately 500 ms in increasing concentrations (left) and a cumulative concentration response curve for glutamate was constructed for 8 cells (right). The fast activation of GluA2 could be captured at higher concentrations (inset; 1 mM).
CaV2.2 - Current-to-Voltage Relationship
Port-a-Patch data and applications:
Cells were kindly provided by Millipore.
Representative current responses of an individual HEK-293 cell expressing CaV2.2 to a standard voltage protocol. Average current-voltage relationship (n = 10). The error bars reflect the standard error of the mean (S.E.M.).
hERG - Pharmacological Experiments at 35 °C
Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore, UK.
A full dose response curve of quinidine acting on the hERG channel was obtained at physiological temperature (35 °C). The IC50 for quinidine at physiological temperature was 1.3 ± 0.2 μM (n = 5), similar to that obtained at room temperature (1.0 ± 0.03 μM, n = 3).
TRPV1 - Heat Activation
Port-a-Patch data and applications:
Raw traces of TRPV1 current responses to voltage ramps from –100 mV up to +100 mV. TRPV1 current at RT (28°C), during two stimulations by application of heated solution (34°C and 36°C), and after cooling down to RT (29°C). The picture on the right-hand side shows the current amplitudes as recorded at -100 mV and +100 mV plotted against time.
KV1.3 - Voltage Induced Membrane Movements Measured with Atomic Force Microscopy
Port-a-Patch data and applications:
Data are taken from Pamir E., et al., Ultramicroscopy, 2008, 108, 552-557.
Voltage induced membrane movements of a Jurkat cell. (a) Deflection signal of the cantilever resting on the cell membrane at an indenting force of 1.0 nN. (b) Corresponding whole cell current: The characteristic response of the voltage gated potassium channel KV1.3 in Jurkat cells is observed. (c) Below: Applied pulse protocol.
KV1.3 - Internal Application of TEA and Quinidine
Port-a-Patch data and applications:
KV1.3 current was blocked by the internal application of increasing concentrations of quinidine (left) or TEA+ (right). For quinidine the IC50 was determined as 15 μM (literature value external application 14 μM) and for TEA+ the IC50 was determined as 0.9 ± 0.3 mM (n = 3) (literature value internal application 0.6 mM).
Gramicidin - Rapid Internal Solution Exchange
Port-a-Patch and
Vesicle Prep Pro data and applications:
Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.
TRPV1 - Internal Application of Calmodulin
Port-a-Patch data and applications:
TRPV1 currents were elicited by the external application of 20 μM capsaicin. After capsaicin activation, the currents were partially blocked by the internal application of Ca2+-calmodulin (50 μM Ca2+ / 500 nM CaM). TRPV1 channels were expressed in CHO cells.
KCa1.1 (BK) - Intracellular Second Messengers
Port-a-Patch data and applications:
Currents mediated by BK channels expressed in CHO cells were studied using the Internal Perfusion System. Currents were elicited by a voltage step from -80 mV to +80 mV before and after adding an internal solution containing a higher concentration of free Ca2+.
TRPM7 - Current Traces
Port-a-Patch data and applications:
Shown are mTRPM7 (HEK293) raw current responses to a voltage ramp voltage procotol (−100 mV to 100 mV over 200 ms) recorded under control conditions and after subsequent internal perfusion with Mg2+ (left). Initally, currents showed some run-up before they became stable. The arrows in the timecourse on the right mark the timepoints for which the raw current traces are shown.
KV1.3 - Continuous Internal Perfusion
Port-a-Patch data and applications:
KV1.3 currents (blue), endogenously expressed in Jurkat cells, were rapidly blocked by internal perfusion of Cs+ (light blue), and fully recovered after washout with K+ (grey). Internal solution replacement was repeated 19 times and the recording was stable for over 35 minutes, as shown in the lower graph.
Glycine Receptor (GlyRa1) - Pharmacology
Port-a-Patch data and applications:
Cells were kindly provided by AstraZeneca, Sweden.
The Laminar Flow Chamber can also be used for studying glycine receptors. The pharmacology of the hGlyRα1, expressed in a mouse fibroblast cell line (L-tk), was investigated. The EC50 for glycine was determined as 89 ± 2.7 μM (n = 10) which is in accordance with the literature.
GABAA Receptor (a1b2g2) - Reliable Compound Application
Port-a-Patch data and applications:
Ligand dependent activation of GABAA receptors can be recorded with the Laminar Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration dependent activation and desensitization of GABAA (α1β2γ2) receptors was obtained by applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s.
MscL - Pressure Clamp Experiments
Port-a-Patch and
Vesicle Prep Pro data and applications:
The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.
Neural Cells (Primary Mouse Stem Cells) - Current Traces
Port-a-Patch data and applications:
Data courtesy of Dr. Gavin Dawe, National University of Singapore.
Patch clamp recordings from cultured mouse primary neuronal stem cells were made in the whole cell configuration (holding potential −60 mV). Currents were evoked by 500 ms depolarizing voltage pulses, showing outwardly rectifying K+- currents.
Neurons (Primary Hippocampal Granule Cell) - Current Traces
Port-a-Patch data and applications:
Recordings from acutely isolated hippocampal granule cells show BK- and CaV currents. Whole cell currents were obtained by depolarizing voltage pulses from a holding potential of −80 mV.
NaV1.5 - Accurate Voltage Control
Port-a-Patch data and applications:
Cells stably transfected with human SCN5A were kindly provided by Millipore.
To perform recordings of fast events, such as the activation and inactivation of Na currents, it is essential to have accurate voltage control. The image shows currents and I/V characteristics of NaV1.5 expressed in HEK293 cells.
Cardiac Action Potentials - hESC-derived Cells
Port-a-Patch data and applications:
Cells were kindly provided by Geron/GE.
The left picture shows a typical action potential in hESC-derived cardiomyocytes (human embryonic stem cell-derived cardiomyocytes). The traces represent K+- (top left), Ca2+- (bottom left) and Na+-currents (top right). The lower right panel shows the corresponding I/V-relationships.
Cardiac Action Potentials - Cor.At® Cells
Port-a-Patch data and applications:
Cells were kindly provided by Axiogenesis
Cor.At® cardiomyocytes are derived from mouse embryonic stem (ES) cells. Whole cell currents recorded in voltage clamp mode reveal cardiomyocyte-typical ion channels (K+, Ca2+ and Na+). Traces on the lower left show prolongation of the action potential upon application of Dofetilide (1 μM).
Download: Application Note
TRPM8 / TRPV1 - Pharmacology
Port-a-Patch data and applications:
TRPV1 data were kindly supplied by Dr. David Cohen, Oregon Health and Service University, Portland, OR, USA.
TRP channels recorded in HEK293 cells. Menthol (dark blue) activated the TRPM8 channels (shown on the left) and could be inhibited by genistein (blue). TRPV1 channels expressed in HEK293 cells (right) were stimulated by application of capsaicin.
TRPM8 - Activated by Menthol
Port-a-Patch data and applications:
External application of 300 µM menthol activated the TRPM8 channels expressed in HEK293 cells.
CaV3.2 - T-Type Calcium Channels
Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx Millipore.
Shown are raw current traces (top left) and average peak current data (top right) of the current voltage relationship of CaV3.2 (T-type Ca2+- Channel) stably expressed in HEK293 cells. Activation and Inactivation plots were constructed (bottom). Half-activation and half-inactivating potentials were determined as -32 mV and -65 mV, respectively.
CaV3.2 - Channel Blockers
Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx Millipore.
Shown are raw current traces (top) and average dose response curves (bottom) of CaV3.2 (T-type Ca2+- Channel) block by compounds as indicated. CaV3.2 is stably expressed in HEK293 cells. IC50s were 863 nM (Mibefradil), 27 μM (Nifedipine) and 52 μM Amiloride.
hERG - Good Results with "Sticky Compounds"
Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore.
Sticky compounds, such as some hERG blockers, exhibit expected IC50 values with the Port-a-Patch. The concentrations of the half maximal block were: 1.27 nM (astemizole), 8.9 nM (cisapride), 163.7 nM (flunarizine) and 11.0 nM (terfenadine).
Bilayer Recordings - Low Noise
Port-a-Patch and
Vesicle Prep Pro data and applications:
Typical recording of the Irms noise level of a bilayer (DPhPC) on the Low Capacitance Holder. Typical values are 220 fA
(10 kHz filter frequency, 50 kHz sampling rate. Cf. 2.3 pA RMS on the standard Port-a-Patch).
hERG - Temperature Control
Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore.
The image shows example traces for hERG mediated currents at 25 ± 2 °C (black) and 35 ± 2 °C (blue). The peak current amplitude was increased at 35 °C, and the rise time and decay time constants were faster at physiological temperature compared with those obtained at room temperature.
Protoplasts (Mesophyll Tabacco Leaves) - Hyperpolarization-Activated Inward Currents
Port-a-Patch data and applications:
Data were kindly provided by Berghöfer; Eing; Flickinger; Frey (Forschungszentrum Karlsruhe, Germany).
Mesophyll protoplasts are enzymatically isolated from tobacco leaves. Shown are whole cell recordings which demonstrate the hyperpolarization-activated inward currents in the protoplasts. A voltage ramp from −40 mV to −120 mV was applied.
KV1 (Shaker-Relared Potassium Channels) - Block of Mutated Channel
Port-a-Patch data and applications:
Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.
Block of Shaker-IR by Agitoxin2. Shown are current recordings from HEK293 transiently expressing Shaker-IR. Currents were elicited by a 500 ms step to +50 mV from a holding potential of -80 mV. K+ gradients are the same as for the IV curve experiment shown above. 200 nM Agitoxin2 almost fully blocks the Shaker K+ current.
Alpha-Hemolysin - DNA Translocation
Port-a-Patch and
Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.
Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch.
TRPM8 - Bilayer Recording
Port-a-Patch and
Vesicle Prep Pro data and applications:
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.
Cytolysin (Bacterial) - Bilayer Recordings
Port-a-Patch and
Vesicle Prep Pro data and applications:
Recordings were kindly supplied by ITC & CNR-Istituto di Biofisica, Trento, Italy.
Traces were recorded in 100 mM KCl, 10 mM Hepes, 0.1 mM EDTA, pH 7 at -40 mV. Lipid: Diphtanoyl-PC.
Alamethicin - Bilayer Recordings
Port-a-Patch and
Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.
Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.
Gramicidin - Single Channel Analysis
Port-a-Patch and
Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.
Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.
Mitochondria - Cell Attached Recordings
Port-a-Patch data and applications:
Mitochondria were isolated from rat cardiac tissue. The patch clamp measurements were performed in the cell attached configuration with an Axopatch 200B. Single channel currents were elicited by a voltage ramp from 0 mV to -80 mV. Due to the cell attached configuration inward currents are displayed as a positive current.
Chloride Channels - Lysosomes Recordings
Port-a-Patch data and applications:
Data are taken from Schieder M. et al., The Journal of Biological Chemistry, 2010, 285(28), 21219-21222.
Typical chloride currents in four isolated lysosomes.Chloride currents were determined as difference currents between IV curves recorded in the absence and presence of extralysosomal high (64 mM) Cl-. On the left a sketch is illustrating the methodology.
Erythrocytes - Single Channel Analysis
Port-a-Patch data and applications:
Erythrocytes lack mitochondria and nuclei and consist mainly of hemoglobin. The membrane contains different ion channels, for example, a Ca2+-activated K+ channel and a volume-sensitive Na+/K+ pump. Here, single channel activity recorded from an erythrocyte in the cell attached configuration is shown.
KCa1.1 (BK) - Single Channel Recordings
Port-a-Patch data and applications:
Single channel recordings of BK channels as recorded from CHO cells in the cell attached mode at +60 mV (top), +40 mV (middle) and 0 mV (lowest trace).
Sonoporation Technology - Contrast Agent Combined with Ultrasound
Port-a-Patch data and applications:
Data were kindly provided by Dr. Cheri Deng, University of Michigan, USA.
Current response of a non-transfected Chinese hamster ovary (CHO) cell to a train of ultrasound pulses (US) in the absence (A) and presence (B) of contrast agent. The holding potential was -80 mV. Ultrasound signals had a centre frequency of 5 MHz. Pulses of 50 cycles were repeated for 2 s every 10 ms.
BY2 Protoplasts - Current Traces
Port-a-Patch data and applications:
Tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells are the most widely used plant cell culture. Depolarisation-activated outward currents in BY2 cell protoplasts. Shown are whole cell current in response to voltage steps (left) and the corresponding IV curve (right). From a holding potential of −40 mV, 500 ms voltage steps were applied from −60 mV to 60 mV in 20 mV increments, with 3 s intervals.
Cardiomyocytes (Ventricular Myocytes) - Recordings
Port-a-Patch data and applicatons:
Thanks to S. Rakovic and D. Terrar from University of Oxford, for preparing the cardiomyocytes used in these experiments.
Ca2+ current recorded from a ventricular myocyte. Raw current voltage relationship and peak current data are plotted (top). From the IV-plot the half-activating voltage was determined as -16 mV. The current was later blocked by the L-type Ca2+ channel blocker, nifedepine (10 µM). Currents were elicited by stepping to +10 mV for 200 ms from a holding potential of -40 mV every 5 seconds.
Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings
Port-a-Patch and
Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.
Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.
TRPV1 - External Perfusion of Capsaicin
Port-a-Patch data and applications:
Data were kindly provided by David Cohen, Oregon Health & Science University, Portland, USA.
Whole cell current responses from HEK 293 cells transiently expressing TRPV1 to a ramp voltage protocol (‑100 mV to +100 mV). Capsaicin at a concentration of 2 μM reversibly activated the channel.
P2X3 - Application of ATP
Port-a-Patch data and applications:
Cells were kindly supplied by Evotec AG, Hamburg, Germany
Shown are raw current traces from 1321 N1 cells expressing P2X3 receptors demonstrating the reproducability of the whole cell responses. Currents were activated by 100 ms application of 10 µM ATP. The waiting time between sweeps was 120 s (holding −60 mV).
NBC1 (Electrogenic Na+/HCO3- Cotransporter) - Current responses
Port-a-Patch data and applications:
Data were kindly provided by Ira Kurtz, UCLA, USA.
Recordings from HEK 293 cells expressing the electrogenic Na+/bicarbonate cotransporter (NBC1). Current responses to 200 ms voltage ramps in high external Na+ initially after establishment of the whole cell configuration (control), after application of HCO3- (bicarbonate), and after wash-out (wash-out).
Kir Channels - Response to Different External Potassium Concentrations
Port-a-Patch data and applications:
Current response in RBL cells to a voltage ramp from –150 mV to +80 mV in the presence of a low and a high external K+ concentration (left). Voltage dependent block of the inward K+ current after the external additon of 50 µM Ba2+ (middle). Same block in response to a continuous voltage step to –150 mV (right) from 0 mV. Low K+ (4.5 mM K+), high K+ (143 mM K+).
KV1 (Shaker-related Potassium Channels) - Mutation without Inactivation
Port-a-Patch data and applications:
Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.
Here we show recordings from HEK293 cells transiently expressing a Shaker K mutant that has its inactivation removed (Shaker-IR). The holding potential was -80 mV. The current-voltage relationship of the tail currents reveals a half-activating potential of -27 mV.
IMR32 Differentiated Cells - Sodium and Potassium Currents
Port-a-Patch data and applications:
Whole cell current responses of undifferentiated (left) and for 12 days differentiated (right) IMR32 cells to a voltage step protocol (holding −90 mV, stepping for 20 ms from −60 mV to +60 mV in 20 mV increments) are shown. The change in current shape indicating increased expression of KV and NaV are apparent.
Potassium Channels - Currents Measured in Differentiated IMR32 Cells
Port-a-Patch data and applications:
Whole cell current responses of undifferentiated (a) and for 12 days differentiated (b) IMR32 cells to a voltage step protocol (holding −90 mV, stepping for 500 ms from −60 mV to +60 mV in 10 mV increments) are shown. In (c) and (d) the corresponding current voltag relationships are shown (closed circles). Open circles are the correspondig current voltage relationships after the cells have been exposed to internal Cs+.
EAG1 (hKv10.1) - Internal Application of TEA
Port-a-Patch data and applications:
Data were kindly provided by Walter Stühmer, MPI for exp. Med., Göttingen.
Block of hEAG whole cell currents after the internal application of 200 µM TEA.
KV1.3 - Perforated Patch
Port-a-Patch data and applications:
Perforated and conventional whole cell configuration derived hKV1.3-currents. Voltage-dependence was shifted significantly to more negative potentials in the whole cell configuration compared to the perforated whole cell configuration. Whole cell currents after a conventional membrane breakthrough (right) and perforated whole cell currents (left).
CNG - cAMP-Regulation of a Plant Ion Channel
Port-a-Patch data and applicatons:
Data were kindly provided by A. Kugler & P. Dietrich, Univ. Erlangen, Germany.
The image shows internal cAMP-activation of a transiently expressed plant CNG channel and subsequent external block by lanthanum. Activators and blockers were manually added to the intracellular and extracellular sides of the membrane.
推荐与案例研究
Prof. Dr. Armin Kargol - Statement about the Port-a-Patch
“Nanion’s Port-a-Patch is an excellent instrument for research labs working with undergraduate students. Manual patch-clamping methods depend to a large extend on the skills of the operator. They require long training periods. For a typical undergraduate student it takes over a year to learn patch-clamping. Port-a-Patch reduces that time to only weeks and the most difficult part is to understand the software that controls the instrument. This allows undergraduate students to actively participate in real research projects.”
Prof. Dr. Armin Kargol, Professor of Physics, Rev. James C. Carter, S.J., Distinguished Professor in Experimental Physics, Department Chair
Loyola University New Orleans, USA
Download the full customer case study here:
Port-a-Patch Customer Case Study (2016)
(0.7 MB)
Prof. Dr. Armin Kargol, Prof. Dr. Kimberlee Mix, Loyola University New Orleans, USA
Prof. Dr. Carlos Villalobos - Statement about the Port-a-Patch
“We have used Nanion´s Port-a-patch for recording endogenous Icrac, the small calcium-release activated currents that are becoming very important in cell fate and cancer hallmarks. In addition, measurements were carried out in primary cultures of human, coronary, vascular-smooth muscle cells, a very difficult cell type to patch, and several normal and tumoral, epithelial cells. In the beginning I was very skeptical, because the currents are extremely small. However, the results were excellent and clear cut. It is possible to investigate the pharmacology and molecular biology of these channels during the phenotypic switches related to transdifferentiation, aging or tumorogenesis”.
Prof. Carlos Villalobos
University of Valladolid and Spanish Research Council (CSIC), Spain
Prof. Dr. Friedrich Simmel - Statement about the Port-a-Patch
“The Port-a-Patch enables students and young researchers lacking formal training in electrophysiology to rapidly generate research grade data. Within the courses given at the Institute for Biomolecular Systems and Bioelectronics, the students gain hands-one xperience in basic membrane biophysics and electrophysiology, which is of great value for their education.”
Professor Dr. Friedrich Simmel
Technical University of Munich, Munich, Germany
Prof. Dr. Colin Taylor - Statement about the Port-a-Patch and Vesicle Prep Pro
“We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.”
Colin Taylor, Professor of Cellular Pharmacology, Department of Pharmacology
University of Cambridge, Cambridge, UK
Dr. Chris Fanger - Statement about the Port-a-Patch
“The Port-a-Patch is a state-of-the-art automated patch clamp platform that allows trainees at Hydra without prior experience in electrophysiology to quickly learn how to generate high quality patch clamp recordings. It substantially speeds up the teaching process as well as giving us an easy-to-use platform for efficient accurate screening of ion channel active compounds.”
Chris Fanger, Director of Lead Discovery
Hydra Biosciences, Boston, MA, USA
Dr. Marisa Rangel - Statement about the Vesicle Prep Pro and Port-a-Patch
“Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro setup. Together with the Port-a-Patch, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings.”
Marisa Rangel, PhD, Researcher
University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil, WI, US
网络研讨会
14.10.2020 | Webinar: Measurement of Transient receptor potential cation (TRP) channels using the Patchliner and Port-a-Patch
Port-a-Patch and
Patchliner Webinar
Date: October 14. 2020
Speakers:
Dr. András Horváth (Nanion Technologies; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
14.10.2020 | Webinar: TRP channels in human corneal cells and their role in ophthalmology
Port-a-Patch Webinar
Date: October 14. 2020
Speakers:
Prof. Dr. Stefan Mergler (Charité - Universitätsmedizin Berlin; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
20.11.2018 | Webinar: The RELEVANCE of ion channel interplay – Voltage-activated channels in non-excitable cells
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i),
Patchliner,
Port-a-Patch Webinar
Date: November 20. 2018, 4:00 PM CET (11:00 AM EDT)
The Webinar focuses on the automated patch clamp assay development for the study of red blood cells in health and disease and the RELEVANCE project, an international consortium of 13 partners from academia, diagnostic labs, blood supply centers, and small companies that combines basic and translational research to improve prognostic, diagnostic and therapeutic approaches on red blood cell function in health and disease. To this end, Nanion contributes assays for the electrophysiological characterization of healthy and patient-derived red blood cells.
07.10.2015 | Webinar: Patch Clamp Made Easy, Fast Track to Excellence
Port-a-Patch
This webinar shows applications that go way beyond possibilities of conventional patch-clamping, where the Port-a-Patch facilitates completely novel scientific directions.
下载:
应用文献
Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"
Port-a-Patch application note:
(0.5 MB)
Cardiomyocytes - "Action Potentials in Mouse ES Cell-Derived Cor.At Cardiomyocytes on Nanion´s Port-a-Patch"
Port-a-Patch application note:
(0.6 MB)
Cells were kindly provided by Axiogenesis.
CaV2.2 - "Characterization of CaV2.2 on Nanion's Port-a-Patch"
Port-a-Patch application note:
(0.6 MB)
Cells were kindly provided by Millipore.
CaV3.2 - "Characterization of CaV3.2 on Nanion's Port-a-Patch"
Port-a-Patch application note:
(0.7 MB)
Cells were kindly provided by Millipore.
hERG - "Characterization of hERG (CHO) on Nanion's Port-a-Patch"
Port-a-Patch application note:
(0.6 MB)
Cells were kindly provided by Millipore.
hERG - "Characterization of hERG (HEK293) on Nanion's Port-a-Patch"
Port-a-Patch application note:
(0.7 MB)
Cells were kindly provided by Millipore.
hERG - "Pharmacology of hERG recorded on Nanion´s Port-a-Patch"
Port-a-Patch application note:
(0.4 MB)
Cells were kindly provided by Millipore.
hERG - "Temperature controlled hERG recordings on the Port-a-Patch"
Port-a-Patch application note:
(0.5 MB)
Cells were kindly provided by Millipore.
KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"
Port-a-Patch and
Vesicle Prep Pro application note:
(0.2 MB)
KV1.3 - "Automated internal perfusion of Jurkat cells expressing KV1.3 channels"
Port-a-Patch application note:
(0.2 MB)
Mitochondria - "Mitoplasts from HEK cells on Nanion‘s Port-a-Patch"
Port-a-Patch application note
(0.5 MB)
Mitochondria - "Recordings from mitochondria and mitoplasts"
Port-a-Patch application note
(0.4 MB)
MscL - "Lipid bilayer recordings of a mechanosensitive channel, MscL, using Nanion’s pressure clampchannel, MscL, using Nanion’s pressure clamp"
Port-a-Patch and
Vesicle Prep Pro application note
(0.3 MB)
NaV1.7 - "Patch clamp recordings of hNaV1.7 on Nanion’s Port-a-Patch"
Port-a-Patch application note
(0.7 MB)
Cells were kindly provided by Anaxon
OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "
Port-a-Patch and
Vesicle Prep Pro application note:
(1.4 MB)
TRPA1 - "TRPA1 activation by allyl isothiocyanate recorded on the Port-a-Patch"
Port-a-Patch application note:
(1.4 MB)
TRPM7 - "TRPM7 activation by internal sequestering of Mg2+ ions recorded on the Port-a-Patch"
Port-a-Patch application note:
(0.8 MB)
TRPM8 - Cold activation of TRPM8 using the External Perfusion System and the Port-a-Patch
Port-a-Patch application note:
(3.63 MB)
TRPM8 / TRPV1 - "TRPV1 and TRPM8 recorded on Nanion's Port-a-Patch"
Port-a-Patch application note:
(0.2 MB)
TRPV4 - Activation and inhibition of TRPV4 using different stimuli on the Port-a-Patch
Port-a-Patch application note:
(3.79 MB)
产品单页与目录
发表文献
2021 - Gating the channel pore of ionotropic glutamate receptors with bacterial substrate binding proteins
Port-a-Patch publication in bioRxiv (2021)
2021 - Active components of Bupleurum chinense and Angelica biserrata showed analgesic effects in formalin induced pain by acting on Nav1.7
Port-a-Patch publication in Journal of Ethnopharmacology (2021)
Authors:
Xu Y., Yu Y., Wang Q., Li W., Zhang S., Liao X., Liu Y., Su Y., Zhao M., Zhang J.
2021 - Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion
Port-a-Patch publication in BBA - Molecular and Cell Biology of Lipids (2021)
Authors:
Li G., Huang D., Bhat O.M., Poklis J.L., Zhang A., Zou Y., Kidd J., Gehr T.W.B., Li P-L.
2020 - Wireless Charging Electrochemiluminescence System for Ionic Channel Manipulation in Living Cells
Port-a-Patch publication in ACS Applied Materials & Interfaces (2020)
Authors:
Peng K., Liu S., Lv F., Fu X., Hussain S., Zhao H., Liu L., Wang S.
2020 - Trp, a conserved aromatic residue crucial to the interaction of a scorpion peptide with sodium channels
Port-a-Patch Publication found in The Journal of Biochemistry (2020)
Authors:
Xu Y., Sun J., Yu Y., Kong X., Meng X., Liu Y., Cui Y., Su Y., Zhao M., Zhang J.
2020 - The structure of the antimicrobial human cathelicidin LL-37 shows oligomerization and channel formation in the presence of membrane mimics
Port-a-Patch and
Vesicle Prep Pro publication in Scientific Reports (2020)
Authors:
Sancho-Vaello E., Gil-Carton D., François P., Bonetti E.J., Kreir M., Pothula K.R., Kleinekathöfer U., Zeth K.
2020 - Purification and initial characterization of Plasmodium falciparum K+ channels, PfKch1 and PfKch2 produced in Saccharomyces cerevisiae
Vesicle Prep Pro and
Port-a-Patch publication in Microbial Cell Factories (2020)
Authors:
Molbaek K., Tejada M., Ricke C.H., Scharff-Poulsen P., Ellekvist P., Helix-Nielsen C., Kumar N., Klaerke D.A., Pedersen P.A.
2020 - New natural agonists of the transient receptor potential Ankyrin 1 (TRPA1) channel
Port-a-Patch publication in Nature Scientific Reports (2020)
Authors:
Legrand C., Merlini J.M., de Senarclens‑Bezençon C., Michlig S.
2020 - Mechanism of non-blocking inhibition of sodium channels revealed by conformation-selective photolabeling
Port-a-Patch pre-publication in BioRxiv (2020)
Authors:
Földi M.C., Pesti K., Zboray K., Hegedűs T., Málnási-Csizmadia A., Lukács P., Mike A.
2020 - Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes
Port-a-Patch and
Vesicle Prep Pro publication in Nature Communications (2020)
Authors:
Debnath M., Chakraborty S., Kumar Y.P., Chaudhuri R., Jana B., Dash J.
2020 - Interferon-γ and high glucose-induced opening of Cx43 hemichannels causes endothelial cell dysfunction and damage
Port-a-Patch publication in Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (2020)
Authors:
Sáez J.C., Contreras-Duarte S., Labra V.C., Santibañez C.A., Mellado L.A., Inostroza C.A., Alvear T.F., Retamal M.A., Velarde V., Orellana J.A..
2020 - Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel
Port-a-Patch and
Vesicle Prep Pro publication in Cell Calcium (2020)
Authors:
Moparthi L., Zygmunt P.M.
2020 - Homocysteine-induced electrical remodeling via the mediation of IP 3 R1/Nav1.5 signaling pathway
Port-a-Patch publication in American Journal of Translational Research (2020)
Authors:
Han L., Wu A., Li Q., Xia Z., Wu Y., Hong K., Xia Z., Li J.
2020 - Hepatitis C Virus p7 Induces Membrane Permeabilization by Interacting with Phosphatidylserine
Port-a-Patch and
Vesicle Prep Pro publication in International Journal of Molecular Sciences (2020)
Authors:
Lee H-R., Lee G.Y., You D-G., Kim H.K., Yoo Y.D.
2020 - Fenamates Inhibit Human Sodium Channel Nav1.2 and Protect Glutamate-Induced Injury in SH-SY5Y Cells
Port-a-Patch publication in Cellular and Molecular Neurobiology (2020)
Authors:
Sun J.F., Zhao M.Y., Xu Y.J., Su Y., Kong X.H., Wang Z.Y.
2020 - Electrophysiological Characterization of Transport Across Outer-Membrane Channels From Gram-Negative Bacteria in Presence of Lipopolysaccharides
Port-a-Patch publication in Frontiers in Angewandte Chemie International Edition Engl. (2020)
Authors:
Wang J., Terrasse R., Bafna J.A., Benier L., Winterhalter M.
2020 - Electrophysiological characterization of transport across outer membrane channels from Gram‐negative bacteria in presence of lipopolysaccharides (LPS)
Port-a-Patch,
Vesicle Prep Pro and
Orbit mini Publication in Angewandte Chemie International Edition (2020)
Authors:
Wang J., Terrasse R., Bafna J.A., Benier L., Winterhalter M.
2020 - Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR
Port-a-Patch and
Vesicle Prep Pro publication in Nature Communications (2020)
Authors:
Iwahasi Y., Toyama Y., Imai S., Itoh H., Osawa M., Inoue M., Shimada I.
2020 - Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells
Port-a-Patch publication in Clinical Science (2020)
Authors:
Chen J., Wang F., Zhou Y., Jiang J., Ksimu S., Zhang X., Li J.Z., Niu J., Wang Q.
2020 - Calcium activates purified human TRPA1 with and without its N-terminal ankyrin repeat domain in the absence of calmodulin
Port-a-Patch and
Vesicle Prep Pro publication in Cell Calcium (2020)
Authors:
Moparthi L., Moparthi S.B., Wenger J., Zygmunt P.M.
2020 - Bioluminescence Methodology for Ion Channel Studies
Port-a-Patch Chapter in Patch Clamp Electrophysiology (2020)
Authors:
Wadsworth P.A., Singh A.K., Nguyen N., Stephan C., Laezza F.
2020 - Automated Patch Clamp in Drug Discovery: major breakthroughs and innovation in the last decade
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i instrument)
Patchliner and
Port-a-Patch publication in Expert Opinion on Drug Discovery (2020)
Authors:
Obergrussberger A., Friis S., Brüggemann A., Fertig N.
2020 - Ascorbate-induced oxidative stress mediates TRP channel activation and cytotoxicity in human etoposide-sensitive and -resistant retinoblastoma cells
Port a Patch publication in Laboratory Investigation (2020)
Authors:
Oronowicz J., Reinhard J., Reinach P.S., Ludwiczak S., Luo H., Salem M.H.O.B., Kraemer M.M., Biebermann H., Kakkassery V., Mergler S.
2020 - Arterial Medial Calcification through Enhanced small Extracellular Vesicle Release in Smooth Muscle-Specific Asah1 Gene Knockout Mice
Port-a-Patch publication in Nature Scientific Reports (2020)
Authors:
Bhat O.M., Li G., Yuan X., Huang D., Gulbins E., Kukreja R.C., Li P-L.
2020 - Addressing hERG activity while maintaining favorable potency, selectivity and pharmacokinetic properties of PPARδ modulators
Port-a Patch Publication in Bioorganic & Medicinal Chemistry Letters (2020)
Authors:
Lagu B., Senaiar R.S., Kluge A.F., Mallesh B., Ramakrishna M., Bhat R., Patane M.A.
2020 - A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy
Vesicle Prep Pro and
Port-a-Patch publication in Communications Biology (2020)
Authors:
Manzo G., Hind C.K., Ferguson P.M., Amison R.T., Hodgson-Casson A.C., Ciazynska K.A., Weller B.J., Clarke M., Lam C., Man R.C.H., O'Shaughnessy B.G., Clifford M., Bui T.T., Drake A.F., Atkinson R.A., Lam J.K.W., Pitchford S.C., Page C.P., Phoenix D.A., Lorenz C.D., Sutton J.M., Mason A.J.
2019 - Temporin L and aurein 2.5 have identical conformations but subtly distinct membrane and antibacterial activities
Port-a-Patch and
Vesicle Prep Pro publication in Nature Scientific Reports (2019)
Authors:
Manzo G., Ferguson P.M., Hind C.H., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton J.M., Lorenz C.D., Phoenix D.A., Mason A.J.
2019 - Structure of the human ClC-1 chloride channel
Port-a-Patch,
Vesicle Prep Pro and
Orbit mini publication in PLOS Biology (2019)
Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.
2019 - Screening by changes in stereotypical behavior during cell motility
Port-a Patch Publication in Scientific Reports (2019)
Authors:
Tweedy L., Witzel P., Heinrich D., Insall R.H., Endres R.G.
2019 - Role of High‐Throughput Electrophysiology in Drug Discovery
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i),
Patchliner and
Port-a-Patch review article in Current Protocols in Pharmacology (2019)
Authors:
Liu C., Li T., Chen J.
2019 - Postpartum hormones oxytocin and prolactin cause pro-arrhythmic prolongation of cardiac repolarization in long QT syndrome type 2
Port-a Patch Publication in EP Europace (2019)
Authors:
Bodi I., Sorge J., Castiglione A., Glatz S.M., Wuelfers E.M., Franke G., Perez-Feliz S., Koren G., Zehender M., Bugger H., Seemann G., Brunner M., Bode C., Odening K.E.
2019 - NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated Cl− current
Port-a Patch Publication in Naunyn-Schmiedeberg's Archives of Pharmacology (2019)
Authors:
Zhong, J., Qin Z., Yu H., Liu X., Li C., Shi J., Mao J., Xu B.
2019 - NMDA Receptor-Mediated Signaling Pathways Enhance Radiation Resistance, Survival and Migration in Glioblastoma Cells-A Potential Target for Adjuvant Radiotherapy
Port-a Patch Publication in Cancers (2019)
Authors:
Müller-Längle A., Lutz H., Hehlgans S., Rödel F., Rau K., Laube B.
2019 - Molecular understanding of calcium permeation through the open Orai channel
Port-a Patch and
Vesicle Prep Pro Publication in PLOS Computational Biology (2019)
Authors:
Liu X., Wu G., Yu Y., Chen X., Ji R., Lu J., Li X., Zhang X., Yang X., Shen Y.
2019 - Modulation of the potassium channel KcsA by anionic phospholipids: Role of arginines at the non-annular lipid binding sites
Port-a Patch Publication in BBA - Biomembranes (2019)
Authors:
Poveda J.A., Giudici A.M., Renart M.L., Millet O., Morales A., González-Ros J.M., Oakes V., Furini S., Domene C
2019 - Mitragynine, an euphoric compound inhibits hERG1a/1b channel current and upregulates the complexation of hERG1a-Hsp90 in HEK293-hERG1a/1b cells
Port-a Patch Publication in Nature Scientific Reports (2019)
Authors:
Tay Y.L., Amanah A., Adenan M.I., Wahab H.A., Tan M.L.
2019 - Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity
Port-a-Patch and
Vesicle Prep Pro publication in Nature Scientific Reports (2019)
Authors:
Manzo G., Ferguson P.M., Gustilo V.B., Ali H., Bui T.T., Drake A.F., Atkinson R.A., Batoni G., Lorenz C.D., Phoenix D.A., Mason A.J.
2019 - KCa1.1 and Kv1.3 channels regulate the interactions between fibroblast-like synoviocytes and T lymphocytes during rheumatoid arthritis
Port-a-Patch publication in Arthritis Research & Therapy (2019)
Authors:
Tanner M.R., Pennington M.W., Chauhan S.S., Laragione T., Gulko P.S., Beeton C.
2019 - Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells
Port-a-Patch publication in Cancers (2019)
Authors:
Gutiérrez L.G., Hernández-Morales M., Núñez L., Villalobos C.
2019 - High-resolution experimental and computational electrophysiology reveals weak β-lactam binding events in the porin PorB
Port-a-Patch publication in Nature Scientific Reports (2019)
Authors:
Bartsch A., Llabrés S., Pein F., Kattner C., Schön M., Diehn M., Tanabe M., Munk A., Zachariae U., Steinem C.
2019 - Golgi anti-apoptotic proteins are evolutionarily conserved ion channels that regulate cell death in plants
Port-a-Patch and
Vesicle Prep Pro Pre-publication in BioRxiv (2019)
Authors:
Sierla M., Prole D.L., Saraiva N., Carrara G., Dinischiotu N., Vaattovaara A., Wrzaczek M., Taylor C.W., Smith F.L., Feys B.
2019 - Fenamates inhibit human sodium channel Nav1.7 and Nav1.8
Port-a-Patch publication in Neuroscience Letters (2019)
Authors:
Sun, J-F., Xu, Y-J., Kong, X-H., Su, Y., Wang, Z-Y.
2019 - Control of Lysosomal TRPML1 Channel Activity and Exosome Release by Acid Ceramidase in Mouse Podocytes
Port-a Patch Publication in American Journal of Physiology Cell Physiology (2019)
Authors:
Li G., Huang D., Hong J., Bhat O.M., Yuan X., Li P.L.
2019 - Breaching the Barrier: Quantifying Antibiotic Permeability across Gram-Negative Bacterial Membranes
Port-a Patch Publication in Journal of Molecular Biology (2019)
Authors:
Cama J., MaeHenney A., Winterhalter M.
2019 - Action of MK‐7264 (Gefapixant) at human P2X3 and P2X2/3 receptors and in vivo efficacy in models of sensitisation
Port-a-Patch publication in British Journal of Pharmacology (2019)
Authors:
Richards D., Gever J.R., Ford A.P., Fountain S.J.
2019 - Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures
Port-a Patch and Internal Perfusion Publication in JCI Insight (2019)
Authors:
Hammelmann, V., Stieglitz, M.S., Hülle, H., Le Meur, K., Kass, J., Brümmer, M., Gruner, C., Rötzer, R.D., Fenske, S., Hartmann, J., Zott, B., Lüthi, A., Spahn, S., Moser, M,, Isbrandt, D., Ludwig, A., Konnerth, A., Wahl-Schott, C., Biel M.
2018 - Vascular Endothelial Growth Factor (VEGF) Induced Downstream Responses to Transient Receptor Potential Vanilloid 1 (TRPV1) and 3-Iodothyronamine (3-T1AM) in Human Corneal Keratocytes
Port-a-Patch publication in Frontiers in Pharmacology (2018)
Authors:
Türker, E., Garreis, F., Khajavi, N., Reinach, P.S., Joshi, P., Brockmann, T., Lucius, A., Ljubojevic, N., Turan, E., Cooper, D., Schick, F., Reinholz, R., Pleyer, U., Köhrle, J., Mergler, S.
2018 - TRPM8 Activation via 3-Iodothyronamine Blunts VEGF-Induced Transactivation of TRPV1 in Human Uveal Melanoma Cells
Port-a-Patch publication in Frontiers in Pharmacology (2018)
Authors:
Walcher, L., Budde, C., Böhm, A., Reinach, P.S., Dhandapani, P., Ljubojevic, N., Schweiger, M.W., von der Waydbrink, H., Reimers, I., Köhrle, J., Mergler, S.
2018 - Thymol Induces Conidial Apoptosis in Aspergillus flavus via Stimulating K+ Eruption
Port-a-Patch publication in Journal of Agricultural and Food Chemistry (2018)
Authors:
Hu L.-B., Ban F.-F., Li H.-B., Qian P.-P., Shen Q.-S., Yan-Zhao Y., Mo H.-Z., Zhou X.
2018 - Temperature-Induced Modulation of Voltage-Gated Ion Channels in Human Lung Cancer Cell Line A549 Using Automated Patch Clamp Technology
Port-a-Patch book chapter in World Congress on Medical Physics and Biomedical Engineering 2018.
Authors:
Langthaler S., Bergmoser K., Lassnig A., Baumgartner C.
2018 - Targeting KCa1.1 channels with a scorpion venom peptide for the therapy of rat models of rheumatoid arthritis
Port-a-Patch publication in The Journal of Pharmacology and Experimental Therapeutics (2018)
Authors:
Tanner M.R., Pennington R.W., Chamberlain B.H., Huq R., Gehrmann E.J., Laragione, T., Gulko P.S., Beeton C.
2018 - Structure of the mechanosensitive OSCA channels
Port-a-Patch Suction Control Pro publication in Nature Structural & Molecular Biology (2018)
Authors:
Zhang M., Wang D., Kang Y., Wu J.-X., Yao F., Pan C., Yan Z., Song C., Chen L.
2018 - Single channel recording of a mitochondrial calcium uniporter
Port-a-Patch and
Vesicle Prep Pro publication in BBRC (2018)
Authors:
Wu G., Li S., Zong G., Liu, X., Fei S., Shen L., Guan X., Yanga X., Shen Y.
2018 - Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics
Port-a-Patch and
Vesicle Prep Pro publication in Journal of Cell Biology (2018)
Authors:
Lee G.Y., You D.G., Lee H.R., Hwang S.W., Lee C.J., Do Yoo Y.
2018 - Role of the Fourth Transmembrane Segment in TRAAK Channel Mechanosensitivity
Port-a-Patch Suction Control Pro pre-publication in bioRxiv (2018)
Authors:
Zhang M., Yao F., Pan C., Yan Z.
2018 - Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers
Port-a-Patch and
Vesicle Prep Pro publication in Current Protocols in Pharmacology (2018)
Authors:
Klaerke D.A., de los Angeles Tejada M., Grøsfjeld Christensen V., Lassen M., Amstrup Pedersen P., Calloe K.
2018 - Quantifying Permeation of Small Charged Molecules across Channels: Electrophysiology in Small Volumes
Port-a-Patch and
Vesicle Prep Pro publication in ACS Omega (2018)
Authors:
Wang J., Benier L., Winterhalter M.
2018 - Powering the ABC multidrug exporter LmrA: How nucleotides embrace the ion-motive force
Port-a-Patch publication in Science Advances (2018)
Authors:
Agboh K., Lau C.H.F., Khoo Y.S.K., Singh H., Raturi S., Nair A.V., Howard J., Chiapello M., Feret R., Deery M.J., Murakami S., van Veen H.W.
2018 - Parallel evolution of frog antimicrobial peptides produces identical conformations but subtly distinct membrane and antibacterial activities
Port-a-Patch and
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Manzo G., Ferguson P.M., Hind C., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton M.J., Lorenz C.D., Phoenix D.A., Mason A.J.
2018 - Non-blocking modulation contributes to sodium channel inhibition by a covalently attached photoreactive riluzole analog
Port-a-Patch publication in Nature Scientific Reports (2018)
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2018 - Molecular mechanism of the Orai channel activation
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2018 - Interaction of Gravity with Cellular Compounds
Port-a-Patch book chapter in Gravitational Biology II (2018)
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2018 - Inhibition of pannexin-1 channel activity by adiponectin in podocytes: Role of acid ceramidase activation
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2018 - Functional Analysis of Membrane Proteins Produced by Cell-Free Translation
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2018 - Enhanced Cardiomyocyte NLRP3 Inflammasome Signaling Promotes Atrial Fibrillation
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Yao C., Veleva T., Scott L., Cao S., Li L., Chen G., Jeyabal P., Pan X., Alsina K.M., Abu-Taha I., Ghezelbash S., Reynolds C.L., Shen Y.H., LeMaire S.A., Schmitz W., Müller F.U., El-Armouche A., Eissa N.T., Beeton C., Nattel S. , Wehrens X.H.T., Dobrev D., Li N.
2018 - Electrophysiological experiments in microgravity: lessons learned and future challenges
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2018 - An aromatic cluster in Lysinibacillus sphaericus BinB involved in toxicity and proper in-membrane folding
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2018 - An allosteric mechanism of inactivation in the calcium-dependent chloride channel BEST1
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2018 - 3-Iodothyronamine Activates a Set of Membrane Proteins in Murine Hypothalamic Cell Lines
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2017 - The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel
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Pham T., Perry J.L., Dosey T.L., Delcour L.H.,Hyser J.M.
2017 - Synthetic Channel Specifically Inserts into the Lipid Bilayer of Gram-Positive Bacteria but not that of Mammalian Erythrocytes
Port-a-Patch publication in Angewandte Chemie (2017)
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Zhang M., Zhu P.P., Xin P., Si W., Li Z.T., Hou J.L.
2017 - Prolonged immunomodulation in inflammatory arthritis using the selective Kv1.3 channel blocker HsTX1[R14A] and its PEGylated analog
Port-a-Patch publication in Clinical Immunology (2017)
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2017 - Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction
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Rangel M., Fernandes dos Santos Castro F., Mota-Lima L.D., Clissa P.B., Martins D.B., Perez dos Santos Cabrera M., Mortari M.R.
2017 - Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans
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2017 - Organelle membrane derived patches: reshaping classical methods for new targets
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Shapovalov G., Ritaine A., Bidaux G., Slomianny, C., Borowiec A-S., Gordienko D., Bultynck G., Skryma R., Prevarskaya N.
2017 - Mitochondria sustain store-operated currents in colon cancer cells but not in normal colonic cells: reversal by non-steroidal anti-inflammatory drugs
Port-a-Patch publication in Oncotarget (2017)
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2017 - Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)
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2017 - Cytotoxic effect of zinc oxide nanoparticles on murine photoreceptor cells via potassium channel block and Na+/K+‐ATPase inhibition
Port-a-Patch publication in Cell Proliferation (2017)
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2017 - Buserelin alleviates chloride transport defect in human cystic fibrosis nasal epithelial cells
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Calvez M.L., Benz N., Huguet F., Saint-Pierre A., Rouillé E., Coraux C., Férec C., Kerbiriou M., Trouvé P.
2017 - Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel
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Arya P, Jarerattanachat V., Clausen M.V., Schewe M., McClenaghan C., Argent L., Conrad L.J., Dong Y.Y., Pike A.C.W., Carpenter E.P., Baukrowitz T., Sansom M.S.P., Tucker S.J.
2017 - Asymmetric mechanosensitivity in a eukaryotic ion channel
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2017 - A mutant of the Buthus martensii Karsch antitumor-analgesic peptide exhibits reduced inhibition to hNav1.4 and hNav1.5 channels while retaining analgesic activity
Port-a-Patch publication in Journal of Biological Chemistry (2017)
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Xu Y., Meng X., Hou X., Sun J., Kong X., Sun Y., Liu Z., Ma Y., Niu Y., Song Y., Cui Y., Zhao M., Zhang J.
2016 - Voltage Dependence of Conformational Dynamics and Subconducting States of VDAC-1
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Briones R., Weichbrodt C., Paltrinieri L., Mey I., Villinger S., Giller K., Lange A., Zweckstetter M., Griesinger C., Becker S., Steinem C., de Groot B.L.
2016 - Upregulation of Transient Receptor Potential Vanilloid Type-1 Channel Activity and Ca2+ Influx Dysfunction in Human Pterygial Cells
Port-a-Patch publication in Investigative Opthalmology & Visual Science (2016)
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2016 - The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor
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2016 - Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola
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2016 - Reversible control of current across lipid membranes by local heating
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2016 - Pore architecture of TRIC channels and insights into their gating mechanism
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2016 - Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1
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Babes A., Sauer S.K., Moparthi L., Kichko T.I., Neacsu C., Namer B., Filipovic M., Zygmunt P.M., Reeh P.W., Fischer M.J.
2016 - Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity
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2016 - Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system
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2016 - Distinct regions that control ion selectivity and calcium-dependent activation in the bestrophin ion channel
Port-a-Patch publication in Proceedings of the National Academy of Sciences of the United States of America (2016)
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2016 - Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium
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2016 - Coupling Data Mining and Laboratory Experiments to Discover Drug Interactions Causing QT Prolongation
Patchliner publication in Journal of the American College of Cardiology (2016)
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Lorberbaum T., Sampson K.J., Chang J.B., Iyer V., Woosley R.L., Kass R.S., Tatonetti N.P.
2016 - Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity
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Vesicle Prep Pro publication in Journal of Biomedical Science and Engineering (2016)
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Sieber M., Kaltenbach S., Hanke W. and Kohn F.P.M
2015 - The Functional Property Changes of Muscular Nav1.4 and Cardiac Nav1.5 Induced by Scorpion Toxin BmK AGP-SYPU1 Mutants Y42F and Y5F
Port-a-Patch publication in Biochemistry (2015)
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2015 - The application of the Escherichia coli giant spheroplast for drug screening with automated planar patch clamp system
Port-a-Patch publication in Biotechnology Reports (2015)
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Kikuchi K., Sugiura M., Nishizawa-Harada C., Kimura T.
2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli
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2015 - K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac
Port-a-Patch publication in Science (2015)
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Dong Y.Y., Pike A.C., Mackenzie A., McClenaghan C., Aryal P., Dong L., Quigley A., Grieben M., Goubin S., Mukhopadhyay S., Ruda G.F., Clausen M.V., Cao L., Brennan P.E., Burgess-Brown N.A., Sansom M.S., Tucker S.J., Carpenter E.P.
2015 - Golgi anti-apoptotic proteins are highly conserved Ion Channels That Affect Apoptosis and Cell Migration
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Carrara G., Saraiva N., Parsons M., Byrne B., Prole D.L., Taylor C.W., Smith G.L.
2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching
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Billen B., Brams M., Debaveye S., Remeeva A., Alpizar Y.A., Waelkens E., Kreir M., Brüggemann A., Talavera K., Nilius B., Voets T., Ulens C.
2015 - Cell-free expression of a functional pore-only sodium channel
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2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms
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2014 - Ultra-stable glass microcraters for on-chip patch clamping
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Stava E., Shin H.C.,Yu M., Bhat A., Resto P.J., Seshadri A., Williams J.C., Blick R.H.
2014 - Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes
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2014 - Studying mechanosensitive ion channels with an automated patch clamp
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2014 - Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins
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2014 - Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system
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2014 - L-Carnitine Reduces in Human Conjunctival Epithelial Cells Hypertonic- Induced Shrinkage through Interacting with TRPV1 Channels
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2014 - KCNMA1 Encoded Cardiac BK Channels Afford Protection against Ischemia-Reperfusion Injury
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Soltysinska E., Bentzen B.H., Barthmes M., Hattel H., Thrush A.B., Harper M.E., Qvortrup K., Larsen F.J., Schiffer T.A., Losa-Reyna J., Straubinger J., Kniess A., Thomsen M.B., Brüggemann A., Fenske S., Biel M., Ruth P., Wahl-Schott C., Boushel R.C., Olesen S.P., Lukowski R.
2014 - Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells
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Benz N., Le Hir S., Norez C., Kerbiriou M., Calvez M.-L., Becq F., Trouvé P. , Férec C.
2014 - Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain
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2014 - High susceptibility to fatty liver disease in two-pore channel 2-deficient mice
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Grimm C., Holdt L.M., Chen C.C., Hassan S., Müller C., Jörs S., Cuny H., Kissing S., Schröder B., Butz B., Northoff B., Castonguay J., Luber C.A., Moser M., Spahn S., Lüllmann-Rauch R., Fendel C., Klugbauer N., Griesbeck O., Haas A., Mann M., Bracher F., Teupser D., Saftig P., Biel M., Wahl-Schott C.
2014 - Crystal structure of a human GABAA receptor
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2014 - Calcium regulation by temperature-sensitive transient receptor potential channels in human uveal melanoma cells
Port-a-Patch publication in Cellular Signalling (2014)
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Mergler S., Derckx R., Reinach P.S., Garreis F., Böhm A., Schmelzer L., Skosyrski S., Ramesh N., Abdelmessih S., Polat O.K., Khajavi N., Riechardt A.I.
2014 - Automated Patch Clamp Analysis of nAChα7 and NaV1.7 Channels
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Patchliner publication in Current Protocols in Pharmacology (2014)
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Obergrussberger A., Haarmann C., Rinke I., Becker N., Guinot D., Brueggemann A., Stoelzle-Feix S., George M., Fertig N.
2014 - An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity
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Danilchanka A., Sun J., Pavlenok M., Maueröder C., Speer A., Siroy A., Marrero J., Trujillo C., Mayhew D.L., Doornbos K.S., Muñoz 'L.E., HerrmannM., EhrtS., Berens C., Niederweisa M.
2014 - A small molecule restores function to TRPML1 mutant isoforms responsible for mucolipidosis type IV
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Chen C.C., Keller M., Hess M., Schiffmann R., Urban N., Wolfgardt A., Schaefer M., Bracher F., Biel M., Wahl-Schott C., Grimm C.
2014 - A potent and Kv1.3-selective analogue of the scorpion toxin HsTX1 as a potential therapeutic for autoimmune diseases
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Rashid M.H., Huq R., Tanner M.R., Chhabra S., Khoo K.K., Estrada R., Dhawan V., Chauhan S., Pennington M.W., Beeton C., Kuyucak S., Norton R.S.
2013 - Monoacylglycerols Activate TRPV1 – A Link between Phospholipase C and TRPV1
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Zygmunt P.M., Ermund A., Movahed P., Andersson D.A., Simonsen C., Jönsson B.A., Blomgren A., Birnir B., Bevan S., Eschalier A., Mallet C., Gomis A., Högestätt E.D.
2013 - Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells
Port-a-Patch publication in Experimental Eye Research (2013)
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Mergler S., Mertens C., Valtink M., Reinach P.S., Castelo Székely V., Slavi N., Abdelmessih S., Türker E., Fels G., Pleyer U.
2013 - Differential Effects of the β‐Adrenoceptor Blockers Carvedilol and Metoprolol on SQT1‐ and SQT2‐Mutant Channels
Port-a-Patch publication in Journal of Cardiovascular Electrophysiology (2013)
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Bodi I., Franke G., Pantulu N.D., Wu K., Perez-Feliz S., Bode C., Zehender M., Zur Hausen A., Brunner M., Odening K.
2013 - Crystal structure and functional mechanism of a human antimicrobial membrane channel
Port-a-Patch publication in Proceedings of the National Academy of Sciences of the United States of America (2013)
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Songa C., Weichbrodt C., Salnikov E.S., Dynowski M.,Forsberg B.O., Bechinger B., Steinem C., de Groot B.L., Zachariae U. and Zeth K.
2013 - A Potent and Selective Peptide Blocker of the Kv1.3 Channel: Prediction from Free-Energy Simulations and Experimental Confirmation
Port-a-Patch publication in PLoS ONE (2013)
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Rashid M.H., Heinzelmann G., Huq R., Tajhya R.B., Chang S.C., Chhabra S., Pennington M.W., Beeton C., Norton R.S., Kuyucak S.
2012 - Thermo-sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells
Port-a-Patch publication in Cellular Signalling (2012)
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Mergler S., Skrzypski M., Sassek M., Pietrzak P., Pucci C., Wiedenmann B., Strowski M.Z.
2012 - Selective modification of the N-Terminal structure of polytheonamide B significantly changes its cytotoxicity and activity as an ion channel
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2012 - Regulation of Hyperpolarization-activated Cyclic Nucleotide-gated (HCN) Channel Activity by cCMP
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Zong X., Krause S., Chen C.C., Krüger J., Gruner C., Cao-Ehlker X., Fenske S., Wahl-Schott C., Biel M.
2012 - Natural and artificial ion channels for biosensing platforms
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2012 - Isolation, characterization and total regioselective synthesis of the novel μO-conotoxin MfVIA from Conus magnificus that targets voltage-gated sodium channels
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Vetter I., Dekan Z., Knapp O., Adams D.J., Alewood P.F., Lewis R.J.
2012 - Interleukin-1R3 mediates interleukin-1–induced potassium current increase through fast activation of Akt kinase
Port-a-Patch publication in Proceedings of the National Academy of Sciences of the Unites States of America (2012)
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Qian J., Zhu L., Li Q., Belevych N., Chen Q., Zhao F., Herness S., Quan N.
2012 - Insulin-secreting INS-1E cells express functional TRPV1 channels
Port-a-Patch publication in Islets (2012)
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Fågelskiöld A.J., Kannisto K., Boström A., Hadrovic B., Farre C., Eweida M., Wester K., Islam MD.S.
2012 - HTS techniques for patch clamp-based ion channel screening - economy and advances
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SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Expert Opinion on Drug Discovery (2012)
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2012 - Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayer
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Carnarius C., Kreir M., Krick M., Methfessel C., Moehrle V., Valerius O., Brüggemann A., Steinem C., Fertig N.
2012 - Effects of the Antitussive Drug Cloperastine on Ventricular Repolarization in Halothane-Anesthetized Guinea Pigs
Port-a-Patch publication in Journal of Pharmacologigal Sciences (2012)
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Takahara A., Fujiwara K., Ohtsuki A., Oka T., Namekata I., Tanaka H.
2012 - DNA unzipping and protein unfolding using nanopores
Port-a-Patch publication in Methods of Molecular Biology (2012)
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Merstorf C., Cressiot B., Pastoriza-Gallego M., Oukhaled A.G., Bacri L., Gierak J., Pelta J., Auvray L., Mathé J.
2012 - Design, synthesis and functional analysis of dansylated polytheonamide mimic: an artificial Peptide ion channel
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2012 - Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells
Port-a-Patch publication in Histochemistry and Cell Biology (2012)
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Mergler S., Garreis F., Sahlmüller M., Lyras E.-M., Reinach P.S., Dwarakanath A., Paulsen F., Pleyer U.
2012 - Alternative splicing of the N-terminal cytosolic and transmembrane domains of P2X7 controls gating of the ion channel by ADP-ribosylation
Port-a-Patch publication in PLoS ONE (2012)
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Schwarz N., Drouot L., Nicke A., Fliegert R., Boyer O., Guse A.H., Haag F., Adriouch S., Koch-Nolte F.
2011 - Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins
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Shaya D., Kreir M., Robbins R.A., Wong S., Hammon J., Brüggemann A., Minor Jr. D.L.
2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells
Port-a-Patch publication in Journal of Cellular Physiology (2011)
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Mergler S., Garreis F., Sahlmüller M., Reinach P.S., Paulsen F., and Pleyer U.
2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening
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SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)
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2011 - Physiology of the Human Corneal Endothelium – New Insights from Electrophysiological Investigations
Port-a-Patch publication in Klinische Monatsblätter für Augenheilkunde (2011)
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2011 - Functional Analysis of Synthetic Substructures of Polytheonamide B: A Transmembrane Channel-Forming Peptide
Port-a-Patch publication in Angewandte Chemie International Edition (2011)
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Matsuoka S., Shinohara N., Takahashi T., Iida M., Inoue M.
2011 - Chemical and biological characterization of four new linear cationic a-helical peptides from the venoms of two solitary eumenine wasps
Port-a-Patch publication in Toxicon (2011)
Authors:
Rangel M., dos Santos Cabrera M.P., Kazuma K., Ando K., Wang X., Kato M., Nihei K., HirataI.Y., Cross T.J., Garcia .N., Faquim-Mauro E.L., Franzolin M.R., Fuchino H., Mori-Yasumoto K., SekitaS., Kadowaki M., SatakeM., KonnoK.
2011 - Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells
Port-a-Patch publication in Experimental Eye Research (2011)
Authors:
Mergler S., Valtink M., Taetz K., Sahlmüller M., Fels G., Reinach P.S., Engelmann K., Pleyer U.
2011 - Cardiac Toxicity
Patchliner and
Port-a-Patch book chapter in ADMET for Medicinal Chemists: A Practical Guide (2011)
Authors:
Kettenhofen R., Schwengberg S.
2011 - Bacterial mechanosensitive channels as a paradigm for mechanosensory transduction
Port-a-Patch publication in Cellular Physiology and Biochemistry (2011)
Authors:
Martinac B.
2011 - Automated Patch Clamp on mESC-Derived Cardiomyocytes for Cardiotoxicity Prediction
Patchliner and
Port-a-Patch publication in Journal of Biological Chemistry (2011)
Authors:
Stoelzle S., Haythornthwaite A., Kettenhofen R., Kolossov E., Bohlen H., George M., Brüggemann A., Fertig N.
2010 - TRPV channels mediate temperature-sensing in human corneal endothelial cells
Port-a-Patch publication in Experimental Eye Research (2010)
Authors:
Mergler S., Valtink M., Coulson-Thomas V.J., Lindemann D., Reinach P.S., Engelmann K., Pleyer U.
2010 - Studying mechanosensitive ion channels using liposomes
Port-a-Patch and
Vesicle Prep Pro publication in Methods in Molecular Biology (2010)
Authors:
Martinac B., Rohde P.R., Battle A. R., Petrov E., Pal P, Alexander Foo A.FW, Vásquez V., Huynh T. and Kloda A.
2010 - Renaissance of ion channel research and drug discovery by patch clamp automation
Port-a-Patch,
Patchliner and
SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Future Medical Chemistry (2010)
Authors:
Farre C. and Fertig N.
2010 - Planar Patch Clamp Approach to Characterize Ionic Currents from Intact Lysosomes
Port-a-Patch publication in Science Signaling (2010)
Authors:
Schieder M., Rötzer K., Brüggemann A., Biel M., Wahl-Schott C.
2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level
Port-a-Patch and
Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)
Authors:
Mahendran K.R., Kreir M., Weingart H., Fertig N., Winterhalter M.
2010 - Cor.At Cardiomyocytes: Primary-like Cardiomyocytes for Manual and Automated Electrophysiological Screening
Port-a-Patch and
Patchliner publication in Lonza Resource Notes (2010)
Authors:
Kettenhofen R., Stölzle S.
2010 - Characterization of two pore channel 2 (TPCN2) -mediated Ca2+ currents in isolated lysosomes
Port-a-Patch publication in The Journal of Biological Chemistry (2010)
Authors:
Schieder M., Rötzer K., Brüggemann A., Biel M., Wahl-Schott C.A.
2009 - Transient receptor potential ankyrin 1 antagonists block the noxious effects of toxic industrial isocyanates and tear gases
Port-a-Patch publication in FASEB (2009)
Authors:
Bessac B.F., Sivula M., von Hehn C.A., Caceres A.I., Escalera, J, Jordt S.
2009 - The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes
Port-a-Patch and
Vesicle Prep Pro publication in Journal of Structural Biology (2009)
Authors:
Gaßmann O., Kreir M., Ambrosi C., Pranskevich J., Oshima A, Röling C., Sosinsky G., Fertig N., Steinem C.
2009 - T-type channel blocking properties and antiabsence activity of two imidazo[1,2-b]pyridazine derivatives structurally related to indomethacin
Port-a-Patch publication in Neuropharmacology (2009)
Authors:
Rimoli M.G., Russo E., Cataldi M., Citraro R., Ambrosino P., Melisi D., Curcio A., De Lucia S., Patrignani P., De Sarro G., Abignente E.
2009 - Port-a-Patch and Patchliner: High fidelity electrophysiology for secondary screening and safety pharmacology
Port-a-Patch and
Patchliner publication in Combinatorial Chemistry & High Throughput Screening (2009)
Authors:
Farre C., Haythornthwaite A., Haarmann C., Stoelzle S., Kreir M., George M., Brüggemann A., Fertig N.
2009 - Amyloid-beta-Induced Ion Flux in Artificial Lipid Bilayers and Neuronal Cells: Resolving a Controversy
Port-a-Patch and
Vesicle Prep Pro publication in Neurotoxicity Research (2009)
Authors:
Capone R., Quiroz F.G., Prangkio P., Saluja I., Sauer A.M., Bautista M.R., Turner R.S., Yang J., Mayer M.
2008 - TRPA1 is a major oxidant sensor in murine airway sensory neurons
Port-a-Patch publication in The Journal of Clinical Investigation (2008)
Authors:
Bessac B.F., Sivula M., von Hehn C.A., Escalera J., Cohn L., Jordt S.E.
2008 - Synthesis and biological evaluation of chalcones as inhibitors of the voltage-gated potassium channel Kv1.3
Port-a-Patch publication in Bioorganic & Medicinal Chemistry Letters (2008)
Authors:
Cianci J., Baell J.B., Flynn B.L., Gable R.W., Mould J.A., Paul D., Harvey A.J.
2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes
Port-a-Patch and
Vesicle Prep Pro publication in Lab-on-a-chip (2008)
Authors:
Kreir M., Farre C., Beckler M., George M., Fertig N.
2008 - Planar patch-clamp force microscopy on living cells
Port-a-Patch publication in Ultramicroscopy (2008)
Authors:
Pamir E., George M., Fertig N., Benoit M.
2008 - Planar patch clamp: Advances in electrophysiology
Port-a-Patch book chapter in "Potassium Channels" (2008)
Authors:
Brüggemann A., Farre C., Haarmann C., Haythornthwaite A., Kreir M., Stoelzle S., George M., Fertig N.
2008 - Ion channel screening – automated patch clamp on the rise
Port-a-Patch and
Patchliner publication in Drug Discovery Today (2008)
Authors:
Farre C., George M., Brüggemann A., Fertig N.
2008 - High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology
Port-a-Patch publication in Nature Reviews Drug Discovery (2008)
Authors:
Dunlop J., Bowlby M., Peri R., Vasilyev D., Arias R.
2007 - Planar Patch Clamping
Port-a-Patch and
Patchliner book chapter in "Patch Clamp Analysis – Advanced Techniques", Series: Neuromethods (2007)
Authors:
Behrends, J.C., Fertig, N.
2007 - Electrophysiological assessment of HERG blockade: Comparative study using automated and conventional patch clamp systems
Port-a-Patch publication in Toxicological Letters (2007)
Authors:
Yook Y., Kim J., Cho H., Moon H., Kwak B.
2007 - Automated ion channel screening: patch clamping made easy
Port-a-Patch and
Patchliner publication in Expert Opinion Therapeutic Targets (2007)
Authors:
Farre C., Stoelzle S., Haarman C., George M., Brueggemann A., Fertig N.
2006 - Microchip technology for automated and parallel patch clamp recording
Port-a-Patch and
Patchliner publication in Small Journal (2006)
Authors:
Brüggemann A., Stoelzle S., George M., Behrends J.C., Fertig N.
2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation
Port-a-Patch and
Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)
Authors:
Sondermann M., George M., Fertig N., Behrends J.C.
2005 - The Port-a-Patch: The smallest patch clamp set up for high quality electrophysiology
Port-a-Patch publication in HEKA Impulse (2005)
Authors:
Brüggemann A., George M., Klau M., Beckler M., Steindl J., Behrends J., Fertig N.
2005 - Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles
Port-a-Patch publication in Nano Letters (2005)
Authors:
Kirchner C., Liedl T., Kudera S., Pellegrino T., Munoz J. A., Gaub H.E., Stoelzle S., Fertig N., Parak W.J.
2004 - Ion channel drug discovery and research: The automated Nano-Patch-Clamp technology
Port-a-Patch publication in Current Drug Discovery Technologies (2004)
Authors:
Brüggemann A., George M., Klau M., Beckler M., Steindl J., Behrends J.C., Fertig N.
2003 - Simultaneous optical and electrical recording of single gramicidin channels
Port-a-Patch publication in Biophysical Journal (2003)
Authors:
Borisenko V., Lougheed L., Hesse J., Fuereder-Kitzmueller E., Fertig N., Behrends, J.C., Woolley G.A., Schuetz G.J.
2003 - Microstructured apertures in planar glass substrates for ion channel research
Port-a-Patch publication in Channels and Receptors (2003)
Authors:
Fertig N., Meyer C., Tilke A., George M., Klau M., Sobotta C., Blick R.H., Behrends J.C.
2003 - Lighting up single ion channels
Port-a-Patch publication in Biophysical Journal (2003)
Authors:
Selvin P.R.
2003 - High quality ion channel analysis on a chip with the NPC-technology
Port-a-Patch publication in Assay and Drug Development Technologies (2003)
Authors:
Brüggemann A., George M., Klau M., Beckler M., Steindl J., Behrends J.C., Fertig N.
2002 - Whole cell patch clamp recording performed on a planar glass chip
Port-a-Patch publication in Biophysical Journal (2002)
Authors:
Fertig N., Blick R.H., Behrends J.C
2002 - Patch clamp on a chip
Port-a-Patch publication in Biophysical Journal (2002)
Authors:
Sigworth F.J., Klemic K.G.
2002 - Activity of single ion channel proteins detected with a planar microstructure
Port-a-Patch publication in Applied Physics Letters (2002)
Authors:
Fertig N., Klau M., George M., Blick R.H., Behrends J.C.
2001 - Microstructured glass chip for ion channel electrophysiology
Port-a-Patch publication in Physical review. E, Statistical, nonlinear, and soft matter physics (2001)
Authors:
Fertig N., Meyer C., Blick R.H., Trautmann C., Behrends J.C.
2000 - Stable integration of isolated cell membrane patches in a nanomachined aperture
Port-a-Patch publication in Applied Physics Letters (2000)
Authors:
Fertig N., Tilke A., Blick R.H., Kotthaus J.P., Behrends J.C., ten Bruggencate G.
海报
2015 - Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp
SURFE²R N1 and
SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and
Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015
(1.6 MB)
2015 - The backstage pass to study your favorite TRP channel
Port-a-Patch and
Patchliner and SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and
SyncroPatch 384PE poster, TRP Meeting 2015
(2.2 MB)
2018 - Combining electrophysiology and contractility recordings for more complete assessment of hiPSC-CMs
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i),
Patchliner and
CardioExcyte 96 poster, Europhysiology Meeting 2018
(1.4 MB)
2018 - Expression and pharmacology of GluA2-containing AMPA receptors in cell lines and stem cell-derived neurons
Port-a-Patch,
Patchliner and
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) poster, Europhysiology Meeting 2018
(0.9 MB)