Buffer Solutions - Product Sheet

   SyncroPatch 384,      Patchliner and      Port-a-Patch - Quality-assured buffer solution product sheet:     (2.8 MB)

preview the file here for download

AMPA Receptor (GluA2) - Reproducible Responses

  Port-a-Patch data and applications:
Cells were kindly provided by SB Drug Discovery.

GluA2 reproducibly recorded on the Port-a-Patch. L-glutamate was applied for 500 ms and this was repeated six times in the same cell showing reproducible responses.

AMPA Receptor (GluA2) - Fast Perfusion with the Port-a-Patch

  Port-a-Patch data and applications:
Cells were kindly provided by SB Drug Discovery.

The AMPA receptor (GluA2) was activated by increasing concentrations of glutamate on the Port-a-Patch. L-glutamate was applied for approximately 500 ms in increasing concentrations (left) and a cumulative concentration response curve for glutamate was constructed for 8 cells (right). The fast activation of GluA2 could be captured at higher concentrations (inset; 1 mM).

CaV2.2 - Current-to-Voltage Relationship

   Port-a-Patch data and applications:
Cells were kindly provided by Millipore.

Representative current responses of an individual HEK-293 cell expressing Ca_V2.2 to a standard voltage protocol. Average current-voltage relationship (n = 10). The error bars reflect the standard error of the mean (S.E.M.).

hERG - Pharmacological Experiments at 35 °C

  Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore, UK.

A full dose response curve of quinidine acting on the hERG channel was obtained at physiological temperature (35 °C). The IC₅₀ for quinidine at physiological temperature was 1.3 ± 0.2 μM (n = 5), similar to that obtained at room temperature (1.0 ± 0.03 μM, n = 3).

TRPV1 - Heat Activation

  Port-a-Patch data and applications:

Raw traces of TRPV1 current responses to voltage ramps from –100 mV up to +100 mV. TRPV1 current at RT (28°C), during two stimulations by application of heated solution (34°C and 36°C), and after cooling down to RT (29°C). The picture on the right-hand side shows the current amplitudes as recorded at -100 mV and +100 mV plotted against time.

KV1.3 - Voltage Induced Membrane Movements Measured with Atomic Force Microscopy

  Port-a-Patch data and applications:Data are taken from Pamir E., et al., Ultramicroscopy, 2008, 108, 552-557.

Voltage induced membrane movements of a Jurkat cell. (a) Deflection signal of the cantilever resting on the cell membrane at an indenting force of 1.0 nN. (b) Corresponding whole cell current: The characteristic response of the voltage gated potassium channel K_V1.3 in Jurkat cells is observed. (c) Below: Applied pulse protocol.

KV1.3 - Internal Application of TEA and Quinidine

  Port-a-Patch data and applications:

K_V1.3 current was blocked by the internal application of increasing concentrations of quinidine (left) or TEA⁺ (right). For quinidine the IC₅₀ was determined as 15 μM (literature value external application 14 μM) and for TEA⁺ the IC₅₀ was determined as 0.9 ± 0.3 mM (n = 3) (literature value internal application 0.6 mM).

Gramicidin - Rapid Internal Solution Exchange

   Port-a-Patch and     Vesicle Prep Pro data and applications:

Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.

TRPV1 - Internal Application of Calmodulin

  Port-a-Patch data and applications:

TRPV1 currents were elicited by the external application of 20 μM capsaicin. After capsaicin activation, the currents were partially blocked by the internal application of Ca²⁺-calmodulin (50 μM Ca²⁺ / 500 nM CaM). TRPV1 channels were expressed in CHO cells.

KCa1.1 (BK) - Intracellular Second Messengers

  Port-a-Patch data and applications:

Currents mediated by BK channels expressed in CHO cells were studied using the Internal Perfusion System. Currents were elicited by a voltage step from -80 mV to +80 mV before and after adding an internal solution containing a higher concentration of free Ca²⁺.

TRPM7 - Current Traces

  Port-a-Patch data and applications:

Shown are mTRPM7 (HEK293) raw current responses to a voltage ramp voltage procotol  (−100 mV to 100 mV over 200 ms) recorded under control conditions and after subsequent internal perfusion with Mg²⁺ (left). Initally, currents showed some run-up before they became stable. The arrows in the timecourse on the right mark the timepoints for which the raw current traces are shown.

KV1.3 - Continuous Internal Perfusion

   Port-a-Patch data and applications:

K_V1.3 currents (blue), endogenously expressed in Jurkat cells, were rapidly blocked by internal perfusion of Cs⁺ (light blue), and fully recovered after washout with K⁺ (grey). Internal solution replacement was repeated 19 times and the recording was stable for over 35 minutes, as shown in the lower graph.

Glycine Receptor (GlyRa1) - Pharmacology

  Port-a-Patch data and applications:
Cells were kindly provided by AstraZeneca, Sweden.

The Laminar Flow Chamber can also be used for studying glycine receptors. The pharmacology of the hGlyRα1, expressed in a mouse fibroblast cell line (L-tk), was investigated. The EC₅₀ for glycine was determined as 89 ± 2.7 μM (n = 10) which is in accordance with the literature.

GABAA Receptor (a1b2g2) - Reliable Compound Application

  Port-a-Patch data and applications:

Ligand dependent activation of GABA_A receptors can be recorded with the Laminar Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration dependent activation and desensitization of GABA_A (α1β2γ2) receptors was obtained by applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s.

MscL - Pressure Clamp Experiments

  Port-a-Patch and     Vesicle Prep Pro data and applications:

The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.

Neural Cells (Primary Mouse Stem Cells) - Current Traces

  Port-a-Patch data and applications:
Data courtesy of Dr. Gavin Dawe, National Uni­versity of Singapore.

Patch clamp recordings from cultured mouse primary neuronal stem cells were made in the whole cell configuration (holding potential −60 mV). Currents were evoked by 500 ms depolarizing voltage pulses, showing outwardly rectifying K⁺- currents.

Neurons (Primary Hippocampal Granule Cell) - Current Traces

  Port-a-Patch data and applications:

Recordings from acutely isolated hippocampal granule cells show BK- and Ca_V currents. Whole cell currents were obtained by depolarizing voltage pulses from a holding potential of −80 mV.

NaV1.5 - Accurate Voltage Control

   Port-a-Patch data and applications:
Cells stably transfected with human SCN5A were kindly provided by Millipore.

To perform recordings of fast events, such as the activation and inactivation of Na currents, it is essential to have accurate voltage control. The image shows currents and I/V characteristics of Na_V1.5 expressed in HEK293 cells.

Cardiac Action Potentials - hESC-derived Cells

   Port-a-Patch data and applications:
Cells were kindly provided by Geron/GE.

The left picture shows a typical action potential in hESC-derived cardiomyocytes (human embryonic stem cell-derived cardiomyocytes). The traces represent K⁺- (top left), Ca²⁺- (bottom left) and Na⁺-currents (top right). The lower right panel shows the corresponding I/V-relationships.

Cardiac Action Potentials - Cor.At® Cells

   Port-a-Patch data and applications:
Cells were kindly provided by Axiogenesis

Cor.At^® cardiomyocytes are derived from mouse embryonic stem (ES) cells. Whole cell currents recorded in voltage clamp mode reveal cardiomyocyte-typical ion channels (K⁺, Ca²⁺ and Na⁺). Traces on the lower left show prolongation of the action potential upon application of Dofetilide (1 μM).

Download: Application Note

TRPM8 / TRPV1 - Pharmacology

  Port-a-Patch data and applications: 
TRPV1 data were kindly supplied by Dr. David Cohen, Oregon Health and Service University, Portland, OR, USA.

TRP channels recorded in HEK293 cells. Menthol (dark blue) activated the TRPM8 channels (shown on the left) and could be inhibited by genistein (blue). TRPV1 channels expressed in HEK293 cells (right) were stimulated by application of capsaicin.

TRPM8 - Activated by Menthol

  Port-a-Patch data and applications:

External application of 300 µM menthol activated the TRPM8 channels expressed in HEK293 cells.

CaV3.2 - T-Type Calcium Channels

   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx Millipore.

Shown are raw current traces (top left) and average peak current data (top right) of the current voltage relationship of Ca_V3.2 (T-type Ca²⁺- Channel) stably expressed in HEK293 cells. Activation and Inactivation plots were constructed (bottom). Half-activation and half-inactivating potentials were determined as -32 mV and -65 mV, respectively.

CaV3.2 - Channel Blockers

   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx Millipore.

Shown are raw current traces (top) and average dose response curves (bottom) of Ca_V3.2 (T-type Ca²⁺- Channel) block by compounds as indicated. Ca_V3.2 is stably expressed in HEK293 cells. IC₅₀s were 863 nM (Mibefradil), 27 μM (Nifedipine) and 52 μM Amiloride.

hERG - Good Results with "Sticky Compounds"

   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore.

Sticky compounds, such as some hERG blockers, exhibit expected IC₅₀ values with the Port-a-Patch. The concentrations of the half maximal block were: 1.27 nM (astemizole), 8.9 nM (cisapride), 163.7 nM (flunarizine) and 11.0 nM (terfenadine).

Bilayer Recordings - Low Noise

   Port-a-Patch and     Vesicle Prep Pro data and applications:

Typical recording of the Irms noise level of a bilayer (DPhPC) on the Low Capacitance Holder. Typical values are 220 fA

(10 kHz filter frequency, 50 kHz sampling rate. Cf. 2.3 pA RMS on the standard Port-a-Patch).

hERG - Temperature Control

   Port-a-Patch data and applications:
Cells were kindly provided by Cytomyx/Millipore.

The image shows example traces for hERG mediated currents at 25 ± 2 °C (black) and 35 ± 2 °C (blue). The peak current amplitude was increased at 35 °C, and the rise time and decay time constants were faster at physiological temperature compared with those obtained at room temperature. 

Protoplasts (Mesophyll Tabacco Leaves) - Hyperpolarization-Activated Inward Currents

  Port-a-Patch data and applications:
Data were kindly provided by Berghöfer; Eing; Flickinger; Frey (Forschungszentrum Karlsruhe, Germany).

Mesophyll protoplasts are enzymatically isolated from tobacco leaves. Shown are whole cell recordings which demonstrate the hyperpolarization-activated inward currents in the protoplasts. A voltage ramp from −40 mV to −120 mV was applied.

KV1 (Shaker-Relared Potassium Channels) - Block of Mutated Channel

  Port-a-Patch data and applications: 
Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.

Block of Shaker-IR by Agitoxin2. Shown are current recordings from HEK293 transiently expressing Shaker-IR. Currents were elicited by a 500 ms step to +50 mV from a holding potential of -80 mV. K⁺ gradients are the same as for the IV curve experiment shown above. 200 nM Agitoxin2 almost fully blocks the Shaker K⁺ current.

Alpha-Hemolysin - DNA Translocation

   Port-a-Patch and      Vesicle Prep Pro data and applications:

Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.

Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch.

TRPM8 - Bilayer Recording

  Port-a-Patch and     Vesicle Prep Pro data and applications: 
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA

Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.

Cytolysin (Bacterial) - Bilayer Recordings

   Port-a-Patch and     Vesicle Prep Pro data and applications:
Recordings were kindly supplied by ITC & CNR-Istituto di Biofisica, Trento, Italy.

Traces were recorded in 100 mM KCl, 10 mM Hepes, 0.1 mM EDTA, pH 7 at -40 mV. Lipid: Diphtanoyl-PC.

Alamethicin - Bilayer Recordings

   Port-a-Patch and     Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.

Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.

Gramicidin - Single Channel Analysis

   Port-a-Patch and      Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.

Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.

Mitochondria - Cell Attached Recordings

  Port-a-Patch data and applications:

Mitochondria were isolated from rat cardiac tissue. The patch clamp measurements were performed in the cell attached configuration with an Axopatch 200B. Single channel currents were elicited by a voltage ramp from 0 mV to -80 mV. Due to the cell attached configuration inward currents are displayed as a positive current.

Chloride Channels - Lysosomes Recordings

  Port-a-Patch data and applications:
Data are taken from Schieder M. et al., The Journal of Biological Chemistry, 2010, 285(28), 21219-21222.

Typical chloride currents in four isolated lysosomes.Chloride currents were determined as difference currents between IV curves recorded in the absence and presence of extralysosomal high (64 mM) Cl^-. On the left a sketch is illustrating the methodology.

Erythrocytes - Single Channel Analysis

   Port-a-Patch data and applications:

Erythrocytes lack mitochondria and nuclei and consist mainly of hemoglobin. The membrane contains different ion channels, for example, a Ca²⁺-activated K⁺ channel and a volume-sensitive Na⁺/K⁺ pump. Here, single channel activity recorded from an erythrocyte in the cell attached configuration is shown.

KCa1.1 (BK) - Single Channel Recordings

   Port-a-Patch data and applications:

Single channel recordings of BK channels as recorded from CHO cells in the cell attached mode at +60 mV (top), +40 mV (middle) and 0 mV (lowest trace).

Sonoporation Technology - Contrast Agent Combined with Ultrasound

  Port-a-Patch data and applications:
Data were kindly provided by Dr. Cheri Deng, University of Michigan, USA.

Current response of a non-transfected Chinese hamster ovary (CHO) cell to a train of ultrasound pulses (US) in the absence (A) and presence (B) of contrast agent. The holding potential was -80 mV. Ultrasound signals had a centre frequency of 5 MHz. Pulses of 50 cycles were repeated for 2 s every 10 ms.

BY2 Protoplasts - Current Traces

   Port-a-Patch data and applications:

Tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells are the most widely used plant cell culture. Depolarisation-activated outward currents in BY2 cell protoplasts. Shown are whole cell current in response to voltage steps (left) and the corresponding IV curve (right). From a holding potential of −40 mV, 500 ms voltage steps were applied from −60 mV to 60 mV in 20 mV increments, with 3 s intervals.

Cardiomyocytes (Ventricular Myocytes) - Recordings

   Port-a-Patch data and applicatons:
Thanks to S. Rakovic and D. Terrar from University of Oxford, for preparing the cardiomyocytes used in these experiments.

Ca²⁺ current recorded from a ventricular myocyte. Raw current voltage relationship and peak current data are plotted (top). From the IV-plot the half-activating voltage was determined as -16 mV. The current was later blocked by the L-type Ca²⁺ channel blocker, nifedepine (10 µM). Currents were elicited by stepping to +10 mV for 200 ms from a holding potential of -40 mV every 5 seconds.

Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings

  Port-a-Patch and     Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.

Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.

TRPV1 - External Perfusion of Capsaicin

  Port-a-Patch data and applications:
Data were kindly provided by David Cohen, Oregon Health & Science University, Portland, USA.

Whole cell current responses from HEK 293 cells transiently expressing TRPV1 to a ramp voltage protocol (‑100 mV to +100 mV). Capsaicin at a concentration of 2 μM reversibly activated the channel.

P2X3 - Application of ATP

  Port-a-Patch data and applications:
Cells were kindly supplied by Evotec AG, Hamburg, Germany

Shown are raw current traces from 1321 N1 cells expressing P2X₃ receptors demonstrating the reproducability of the whole cell responses. Currents were activated by 100 ms application of 10 µM ATP. The waiting time between sweeps was 120 s (holding −60 mV).

NBC1 (Electrogenic Na+/HCO3- Cotransporter) - Current responses

  Port-a-Patch data and applications:
Data were kindly provided by Ira Kurtz, UCLA, USA.

Recordings from HEK 293 cells expressing the electrogenic Na⁺/bicarbonate cotransporter (NBC1). Current responses to 200 ms voltage ramps in high external Na⁺ initially after establishment of the whole cell configuration (control), after application of HCO₃- (bicarbonate), and after wash-out (wash-out).

Kir Channels - Response to Different External Potassium Concentrations

  Port-a-Patch data and applications:

Current response in RBL cells to a voltage ramp from –150 mV to +80 mV in the presence of a low and a high external K⁺ concentration (left). Voltage dependent block of the inward K⁺ current after the external additon of 50 µM Ba²⁺ (middle). Same block in response to a continuous voltage step to –150 mV (right) from 0 mV.  Low K⁺ (4.5 mM K⁺), high K⁺ (143 mM K⁺).

KV1 (Shaker-related Potassium Channels) - Mutation without Inactivation

  Port-a-Patch data and applications: 
Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.

Here we show recordings from HEK293 cells transiently expressing a Shaker K mutant that has its inactivation removed (Shaker-IR). The holding potential was -80 mV. The current-voltage relationship of the tail currents reveals a half-activating potential of -27 mV.

IMR32 Differentiated Cells - Sodium and Potassium Currents

   Port-a-Patch data and applications:

Whole cell current responses of undifferentiated (left) and for 12 days differentiated (right) IMR32 cells to a voltage step protocol (holding −90 mV, stepping for 20 ms from −60 mV to +60 mV in 20 mV increments) are shown. The change in current shape indicating increased expression of K_V and Na_V are apparent.

Potassium Channels - Currents Measured in Differentiated IMR32 Cells

  Port-a-Patch data and applications:

Whole cell current responses of undifferentiated (a) and for 12 days differentiated (b) IMR32 cells to a voltage step protocol (holding −90 mV, stepping for 500 ms from −60 mV to +60 mV in 10 mV increments) are shown. In (c) and (d) the corresponding current voltag relationships are shown (closed circles). Open circles are the correspondig current voltage relationships after the cells have been exposed to internal Cs⁺.

EAG1 (hKv10.1) - Internal Application of TEA

   Port-a-Patch data and applications:
Data were kindly provided by Walter Stühmer, MPI for exp. Med., Göttingen.

Block of hEAG whole cell currents after the internal application of 200 µM TEA.

KV1.3 - Perforated Patch

   Port-a-Patch data and applications:

Perforated and conventional whole cell configuration derived hK_V1.3-currents. Voltage-dependence was shifted significantly to more negative potentials in the whole cell configuration compared to the perforated whole cell configuration. Whole cell currents after a conventional membrane breakthrough (right) and perforated whole cell currents (left).

CNG - cAMP-Regulation of a Plant Ion Channel

   Port-a-Patch data and applicatons:
Data were kindly provided by A. Kugler & P. Dietrich, Univ. Erlangen, Germany.

The image shows internal cAMP-activation of a transiently expressed plant CNG channel and subsequent external block by lanthanum. Activators and blockers were manually added to the intracellular and extracellular sides of the membrane.

Prof. Dr. Armin Kargol - Statement about the Port-a-Patch

   “Nanion’s Port-a-Patch is an excellent instrument for research labs working with undergraduate students. Manual patch-clamping methods depend to a large extend on the skills of the operator. They require long training periods. For a typical undergraduate student it takes over a year to learn patch-clamping. Port-a-Patch reduces that time to only weeks and the most difficult part is to understand the software that controls the instrument. This allows undergraduate students to actively participate in real research projects.”

Prof. Dr. Armin Kargol, Professor of Physics, Rev. James C. Carter, S.J., Distinguished Professor in Experimental Physics, Department Chair
Loyola University New Orleans, USA

Download the full customer case study here:

   Port-a-Patch Customer Case Study (2016)      (0.7 MB)
Prof. Dr. Armin Kargol, Prof. Dr. Kimberlee Mix, Loyola University New Orleans, USA

download here

Prof. Dr. Carlos Villalobos - Statement about the Port-a-Patch

   “We have used Nanion´s Port-a-patch for recording endogenous Icrac, the small calcium-release activated currents that are becoming very important in cell fate and cancer hallmarks. In addition, measurements were carried out in primary cultures of human, coronary, vascular-smooth muscle cells, a very difficult cell type to patch, and several normal and tumoral, epithelial cells. In the beginning I was very skeptical, because the currents are extremely small. However, the results were excellent and clear cut. It is possible to investigate the pharmacology and molecular biology of these channels during the phenotypic switches related to transdifferentiation, aging or tumorogenesis”.

Prof. Carlos Villalobos
University of Valladolid and Spanish Research Council (CSIC), Spain

Prof. Dr. Friedrich Simmel - Statement about the Port-a-Patch

   “The Port-a-Patch enables students and young researchers lacking formal training in electrophysiology to rapidly generate research grade data. ­Within ­the ­courses ­given ­at ­the ­Institute ­for Biomolecular ­Systems and ­Bioelectronics, ­the ­students­ gain ­hands-on­e xperience­ in ­basic membrane ­biophysics ­and ­electrophysiology,­ which­ is ­of ­great ­value for their education.”

Professor­ Dr. Friedrich Simmel
­Technical­ University ­of ­Munich, ­Munich, ­Germany

Prof. Dr. Colin Taylor - Statement about the Port-a-Patch and Vesicle Prep Pro

      “We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.”

Colin Taylor, Professor of  Cellular Pharmacology, Department of Pharmacology
University of Cambridge, Cambridge, UK

Dr. Chris Fanger - Statement about the Port-a-Patch

   “The Port-a-Patch is a state-of-the-art automated patch clamp  platform that allows trainees at Hydra without prior experience in  electrophysiology to quickly learn how to generate high quality patch clamp recordings. It substantially speeds up the teaching process as well as giving us an easy-to-use platform for efficient accurate screening of ion channel active compounds.”

Chris Fanger, Director of Lead Discovery
Hydra Biosciences, Boston, MA, USA

Dr. Marisa Rangel - Statement about the Vesicle Prep Pro and Port-a-Patch

      “Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro setup. Together with the Port-a-Patch, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings.”

Marisa Rangel, PhD, Researcher 
University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil, WI, US

20.11.2018 | Webinar: The RELEVANCE of ion channel interplay – Voltage-activated channels in non-excitable cells

   SyncroPatch 384PE (a predecessor model of SyncroPatch 384i),      Patchliner,      Port-a-Patch Webinar

Date: November 20. 2018, 4:00 PM CET (11:00 AM EDT)

The Webinar focuses on the automated patch clamp assay development for the study of red blood cells in health and disease and the RELEVANCE project, an international consortium of 13 partners from academia, diagnostic labs, blood supply centers, and small companies that combines basic and translational research to improve prognostic, diagnostic and therapeutic approaches on red blood cell function in health and disease. To this end, Nanion contributes assays for the electrophysiological characterization of healthy and patient-derived red blood cells.

07.10.2015 | Webinar: Patch Clamp Made Easy, Fast Track to Excellence

   Port-a-Patch

This webinar shows applications that go way beyond possibilities of conventional patch-clamping, where the Port-a-Patch facilitates completely novel scientific directions.

Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"

   Port-a-Patch application note:      (0.5 MB) 

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Cardiomyocytes - "Action Potentials in Mouse ES Cell-Derived Cor.At Cardiomyocytes on Nanion´s Port-a-Patch"

   Port-a-Patch application note:      (0.6 MB)
Cells were kindly provided by Axiogenesis.

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CaV2.2 - "Characterization of CaV2.2 on Nanion's Port-a-Patch"

   Port-a-Patch application note:      (0.6 MB)
Cells were kindly provided by Millipore.  

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CaV3.2 - "Characterization of CaV3.2 on Nanion's Port-a-Patch"

   Port-a-Patch application note:      (0.7 MB)
Cells were kindly provided by Millipore.  

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hERG - "Characterization of hERG (CHO) on Nanion's Port-a-Patch"

   Port-a-Patch application note:      (0.6 MB)
Cells were kindly provided by Millipore.  

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hERG - "Characterization of hERG (HEK293) on Nanion's Port-a-Patch"

   Port-a-Patch application note:      (0.7 MB)
Cells were kindly provided by Millipore.  

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hERG - "Pharmacology of hERG recorded on Nanion´s Port-a-Patch"

   Port-a-Patch application note:      (0.4 MB)
Cells were kindly provided by Millipore.  

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hERG - "Temperature controlled hERG recordings on the Port-a-Patch"

   Port-a-Patch application note:      (0.5 MB)
Cells were kindly provided by Millipore.  

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KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

   Port-a-Patch and      Vesicle Prep Pro application note:     (0.2 MB)

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KV1.3 - "Automated internal perfusion of Jurkat cells expressing KV1.3 channels"

   Port-a-Patch application note:      (0.2 MB) 

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Mitochondria - "Mitoplasts from HEK cells on Nanion‘s Port-a-Patch"

  Port-a-Patch application note      (0.5 MB)

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Mitochondria - "Recordings from mitochondria and mitoplasts"

  Port-a-Patch application note      (0.4 MB)

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MscL - "Lipid bilayer recordings of a mechanosensitive channel, MscL, using Nanion’s pressure clampchannel, MscL, using Nanion’s pressure clamp"

  Port-a-Patch and      Vesicle Prep Pro application note      (0.3 MB)

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NaV1.7 - "Patch clamp recordings of hNaV1.7 on Nanion’s Port-a-Patch"

  Port-a-Patch application note      (0.7 MB)
Cells were kindly provided by Anaxon

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OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "

   Port-a-Patch and      Vesicle Prep Pro application note:      (1.4 MB) 

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TRPA1 - "TRPA1 activation by allyl isothiocyanate recorded on the Port-a-Patch"

   Port-a-Patch application note:     (1.4 MB)

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TRPM7 - "TRPM7 activation by internal sequestering of Mg2+ ions recorded on the Port-a-Patch"

   Port-a-Patch application note:     (0.8 MB)

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TRPM8 - Cold activation of TRPM8 using the External Perfusion System and the Port-a-Patch

   Port-a-Patch application note:      (3.63 MB) 

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TRPM8 / TRPV1 - "TRPV1 and TRPM8 recorded on Nanion's Port-a-Patch"

   Port-a-Patch application note:     (0.2 MB)

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TRPV4 - Activation and inhibition of TRPV4 using different stimuli on the Port-a-Patch

   Port-a-Patch application note:      (3.79 MB) 

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Port-a-Patch - 产品单页

  Port-a-Patch 产品单页:      (1.7 MB)

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Port-a-Patch Accessories Catalog

  Port-a-Patch Accessories Cataolg      (0.3 MB)

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Port-a-Patch 产品彩页 - Internal Perfusion

  Port-a-Patch 产品彩页      (0.8 MB)

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Port-a-Patch 产品彩页 - Suction Control Pro

  Port-a-Patch 产品彩页      (0.2 MB)

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2020 - Wireless Charging Electrochemiluminescence System for Ionic Channel Manipulation in Living Cells

   Port-a-Patch publication in ACS Applied Materials & Interfaces (2020)

Authors:
Peng K., Liu S., Lv F., Fu X., Hussain S., Zhao H., Liu L., Wang S.

2020 - Trp, a conserved aromatic residue crucial to the interaction of a scorpion peptide with sodium channels

   Port-a-Patch Publication found in The Journal of Biochemistry (2020)

Authors:
Xu Y., Sun J., Yu Y., Kong X., Meng X., Liu Y., Cui Y., Su Y., Zhao M., Zhang J. 

2020 - New natural agonists of the transient receptor potential Ankyrin 1 (TRPA1) channel

   Port-a-Patch publication in Nature Scientific Reports (2020)

Authors:
Legrand C., Merlini J.M., de Senarclens‑Bezençon C., Michlig S.

2020 - Mechanism of non-blocking inhibition of sodium channels revealed by conformation-selective photolabeling

   Port-a-Patch pre-publication in BioRxiv (2020)

Authors:
Földi M.C., Pesti K., Zboray K., Hegedűs T., Málnási-Csizmadia A., Lukács P., Mike A.

2020 - Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes

  Port-a-Patch and      Vesicle Prep Pro publication in Nature Communications (2020)

Authors: 
Debnath M., Chakraborty S., Kumar Y.P., Chaudhuri R., Jana B., Dash J.

2020 - Interferon-γ and high glucose-induced opening of Cx43 hemichannels causes endothelial cell dysfunction and damage

   Port-a-Patch publication in Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (2020)

Authors: 
Sáez J.C., Contreras-Duarte S., Labra V.C., Santibañez C.A., Mellado L.A., Inostroza C.A., Alvear T.F., Retamal M.A., Velarde V., Orellana J.A..

2020 - Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel

  Port-a-Patch and      Vesicle Prep Pro publication in Cell Calcium (2020)

Authors: 
Moparthi L., Zygmuntc P.M.

2020 - Homocysteine-induced electrical remodeling via the mediation of IP 3 R1/Nav1.5 signaling pathway

  Port-a-Patch publication in American Journal of Translational Research (2020)

Authors:
Han L., Wu A., Li Q., Xia Z., Wu Y., Hong K., Xia Z., Li J.

2020 - Hepatitis C Virus p7 Induces Membrane Permeabilization by Interacting with Phosphatidylserine

  Port-a-Patch and      Vesicle Prep Pro publication in International Journal of Molecular Sciences (2020)

Authors: 
Lee H-R., Lee G.Y., You D-G., Kim H.K., Yoo Y.D.

2020 - Fenamates Inhibit Human Sodium Channel Nav1.2 and Protect Glutamate-Induced Injury in SH-SY5Y Cells

   Port-a-Patch publication in Cellular and Molecular Neurobiology (2020)

Authors:
Sun J.F., Zhao M.Y., Xu Y.J., Su Y., Kong X.H., Wang Z.Y.

2020 - Electrophysiological Characterization of Transport Across Outer-Membrane Channels From Gram-Negative Bacteria in Presence of Lipopolysaccharides

   Port-a-Patch publication in Frontiers in Angewandte Chemie International Edition Engl. (2020)

Authors:
Wang J., Terrasse R., Bafna J.A., Benier L., Winterhalter M.

2020 - Electrophysiological characterization of transport across outer membrane channels from Gram‐negative bacteria in presence of lipopolysaccharides (LPS)

   Port-a-Patch,     Vesicle Prep Pro and      Orbit mini Publication in Angewandte Chemie International Edition (2020)

Authors:
Wang J., Terrasse R., Bafna J.A., Benier L., Winterhalter M.

2020 - Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells

   Port-a-Patch publication in Clinical Science (2020)

Authors:
Chen J., Wang F., Zhou Y., Jiang J., Ksimu S., Zhang X., Li J.Z., Niu J., Wang Q.

2020 - Calcium activates purified human TRPA1 with and without its N-terminal ankyrin repeat domain in the absence of calmodulin

   Port-a-Patch and      Vesicle Prep Pro publication in Cell Calcium (2020)

Authors: 
Moparthi L., Moparthi S.B., Wenger J., Zygmunt P.M.

2020 - Automated Patch Clamp in Drug Discovery: major breakthroughs and innovation in the last decade

    SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i instrument)      Patchliner and      Port-a-Patch publication in Expert Opinion on Drug Discovery (2020)

Authors:
Obergrussberger A., Friis S., Brüggemann A., Fertig N.

2020 - Ascorbate-induced oxidative stress mediates TRP channel activation and cytotoxicity in human etoposide-sensitive and -resistant retinoblastoma cells

 Port a Patch publication in Laboratory Investigation (2020)

Authors:
Oronowicz J., Reinhard J., Reinach P.S., Ludwiczak S., Luo H., Salem M.H.O.B., Kraemer M.M., Biebermann H., Kakkassery V., Mergler S.

2020 - Arterial Medial Calcification through Enhanced small Extracellular Vesicle Release in Smooth Muscle-Specific Asah1 Gene Knockout Mice

  Port-a-Patch publication in Nature Scientific Reports (2020)

Authors:
Bhat O.M., Li G., Yuan X., Huang D., Gulbins E., Kukreja R.C., Li P-L.

2020 - Addressing hERG activity while maintaining favorable potency, selectivity and pharmacokinetic properties of PPARδ modulators

   Port-a Patch Publication in Bioorganic & Medicinal Chemistry Letters (2020)

Authors:
Lagu B., Senaiar R.S., Kluge A.F., Mallesh B., Ramakrishna M., Bhat R., Patane M.A.

2019 - Temporin L and aurein 2.5 have identical conformations but subtly distinct membrane and antibacterial activities

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports (2019)

Authors:
Manzo G., Ferguson P.M., Hind C.H., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton J.M., Lorenz C.D., Phoenix D.A., Mason A.J.

2019 - Structure of the human ClC-1 chloride channel

   Port-a-Patch,     Vesicle Prep Pro and      Orbit mini publication in PLOS Biology (2019)

Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.

2019 - Screening by changes in stereotypical behavior during cell motility

   Port-a Patch Publication in Scientific Reports (2019)

Authors:
Tweedy L., Witzel P., Heinrich D., Insall R.H., Endres R.G.

2019 - Role of High‐Throughput Electrophysiology in Drug Discovery

   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i),      Patchliner and      Port-a-Patch review article in Current Protocols in Pharmacology (2019)

Authors:
Liu C., Li T., Chen J.

2019 - Postpartum hormones oxytocin and prolactin cause pro-arrhythmic prolongation of cardiac repolarization in long QT syndrome type 2

   Port-a Patch Publication in EP Europace (2019)

Authors:
Bodi I., Sorge J., Castiglione A., Glatz S.M., Wuelfers E.M., Franke G., Perez-Feliz S., Koren G., Zehender M., Bugger H., Seemann G., Brunner M., Bode C., Odening K.E.

2019 - NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated Cl− current

   Port-a Patch Publication in Naunyn-Schmiedeberg's Archives of Pharmacology (2019)

Authors:
Zhong, J., Qin Z., Yu H., Liu X., Li C., Shi J., Mao J., Xu B.

2019 - NMDA Receptor-Mediated Signaling Pathways Enhance Radiation Resistance, Survival and Migration in Glioblastoma Cells-A Potential Target for Adjuvant Radiotherapy

   Port-a Patch Publication in Cancers (2019)

Authors:
Müller-Längle A., Lutz H., Hehlgans S., Rödel F., Rau K., Laube B.

2019 - Molecular understanding of calcium permeation through the open Orai channel

   Port-a Patch and      Vesicle Prep Pro Publication in PLOS Computational Biology (2019)

Authors:
Liu X., Wu G., Yu Y., Chen X., Ji R., Lu J., Li X., Zhang X., Yang X., Shen Y.

2019 - Modulation of the potassium channel KcsA by anionic phospholipids: Role of arginines at the non-annular lipid binding sites

   Port-a Patch Publication in BBA - Biomembranes (2019)

Authors:
Poveda J.A., Giudici A.M., Renart M.L., Millet O., Morales A., González-Ros J.M., Oakes V., Furini S., Domene C

2019 - Mitragynine, an euphoric compound inhibits hERG1a/1b channel current and upregulates the complexation of hERG1a-Hsp90 in HEK293-hERG1a/1b cells

   Port-a Patch Publication in Nature Scientific Reports (2019)

Authors:
Tay Y.L., Amanah A., Adenan M.I., Wahab H.A., Tan M.L.

2019 - Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports (2019)

Authors:
Manzo G., Ferguson P.M., Gustilo V.B., Ali H., Bui T.T., Drake A.F., Atkinson R.A., Batoni G., Lorenz C.D., Phoenix D.A., Mason A.J.

2019 - KCa1.1 and Kv1.3 channels regulate the interactions between fibroblast-like synoviocytes and T lymphocytes during rheumatoid arthritis

   Port-a-Patch publication in Arthritis Research & Therapy (2019)

Authors:
Tanner M.R., Pennington M.W., Chauhan S.S., Laragione T., Gulko P.S., Beeton C.

2019 - Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells

   Port-a-Patch publication in Cancers (2019)

Authors:
Gutiérrez L.G., Hernández-Morales M., Núñez L., Villalobos C.

2019 - High-resolution experimental and computational electrophysiology reveals weak β-lactam binding events in the porin PorB

   Port-a-Patch publication in Nature Scientific Reports (2019)

Authors:
Bartsch A., Llabrés S., Pein F., Kattner C., Schön M., Diehn M., Tanabe M., Munk A., Zachariae U., Steinem C.

2019 - Golgi anti-apoptotic proteins are evolutionarily conserved ion channels that regulate cell death in plants

   Port-a-Patch and      Vesicle Prep Pro Pre-publication in BioRxiv (2019)

Authors:
Sierla M., Prole D.L., Saraiva N., Carrara G., Dinischiotu N., Vaattovaara A., Wrzaczek M., Taylor C.W., Smith F.L., Feys B.

2019 - Fenamates inhibit human sodium channel Nav1.7 and Nav1.8

   Port-a-Patch publication in Neuroscience Letters (2019)

Authors:
Sun, J-F., Xu, Y-J., Kong, X-H., Su, Y., Wang, Z-Y.

2019 - Control of Lysosomal TRPML1 Channel Activity and Exosome Release by Acid Ceramidase in Mouse Podocytes

   Port-a Patch Publication in American Journal of Physiology Cell Physiology (2019)

Authors:
Li G., Huang D., Hong J., Bhat O.M., Yuan X., Li P.L.

2019 - Breaching the Barrier: Quantifying Antibiotic Permeability across Gram-Negative Bacterial Membranes

   Port-a Patch Publication in Journal of Molecular Biology (2019)

Authors:
Cama J., MaeHenney A., Winterhalter M.

2019 - Action of MK‐7264 (Gefapixant) at human P2X3 and P2X2/3 receptors and in vivo efficacy in models of sensitisation

   Port-a-Patch publication in British Journal of Pharmacology (2019)

Authors:
Richards D., Gever J.R., Ford A.P., Fountain S.J.

2019 - Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures

   Port-a Patch and Internal Perfusion Publication in JCI Insight (2019)

Authors:
Hammelmann, V., Stieglitz, M.S., Hülle, H., Le Meur, K., Kass, J., Brümmer, M., Gruner, C., Rötzer, R.D., Fenske, S., Hartmann, J., Zott, B., Lüthi, A., Spahn, S., Moser, M,, Isbrandt, D., Ludwig, A., Konnerth, A., Wahl-Schott, C., Biel M.

2018 - Vascular Endothelial Growth Factor (VEGF) Induced Downstream Responses to Transient Receptor Potential Vanilloid 1 (TRPV1) and 3-Iodothyronamine (3-T1AM) in Human Corneal Keratocytes

   Port-a-Patch publication in Frontiers in Pharmacology (2018)

Authors:
Türker, E., Garreis, F., Khajavi, N., Reinach, P.S., Joshi, P., Brockmann, T., Lucius, A., Ljubojevic, N., Turan, E., Cooper, D., Schick, F., Reinholz, R., Pleyer, U., Köhrle, J., Mergler, S.

2018 - TRPM8 Activation via 3-Iodothyronamine Blunts VEGF-Induced Transactivation of TRPV1 in Human Uveal Melanoma Cells

   Port-a-Patch publication in Frontiers in Pharmacology (2018)

Authors:
Walcher, L., Budde, C., Böhm, A., Reinach, P.S., Dhandapani, P., Ljubojevic, N., Schweiger, M.W., von der Waydbrink, H., Reimers, I., Köhrle, J., Mergler, S.

2018 - Thymol Induces Conidial Apoptosis in Aspergillus flavus via Stimulating K+ Eruption

   Port-a-Patch publication in Journal of Agricultural and Food Chemistry (2018)

Authors:
Hu L.-B., Ban F.-F., Li H.-B., Qian P.-P., Shen Q.-S., Yan-Zhao Y., Mo H.-Z., Zhou X.

2018 - Temperature-Induced Modulation of Voltage-Gated Ion Channels in Human Lung Cancer Cell Line A549 Using Automated Patch Clamp Technology

   Port-a-Patch book chapter in World Congress on Medical Physics and Biomedical Engineering 2018.

Authors:
Langthaler S., Bergmoser K., Lassnig A., Baumgartner C.

2018 - Targeting KCa1.1 channels with a scorpion venom peptide for the therapy of rat models of rheumatoid arthritis

   Port-a-Patch publication in The Journal of Pharmacology and Experimental Therapeutics (2018)

Authors:
Tanner M.R., Pennington R.W., Chamberlain B.H., Huq R., Gehrmann E.J., Laragione, T., Gulko P.S., Beeton C.

2018 - Structure of the mechanosensitive OSCA channels

   Port-a-Patch Suction Control Pro publication in Nature Structural & Molecular Biology (2018)

Authors:
Zhang M., Wang D., Kang Y., Wu J.-X., Yao F., Pan C., Yan Z., Song C., Chen L.

2018 - Single channel recording of a mitochondrial calcium uniporter

   Port-a-Patch and      Vesicle Prep Pro publication in BBRC (2018)

Authors: 
Wu G., Li S., Zong G., Liu, X., Fei S., Shen L., Guan X., Yanga X., Shen Y.

2018 - Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics

   Port-a-Patch and      Vesicle Prep Pro publication in Journal of Cell Biology (2018)

Authors:
Lee G.Y., You D.G., Lee H.R., Hwang S.W., Lee C.J., Do Yoo Y.

2018 - Role of the Fourth Transmembrane Segment in TRAAK Channel Mechanosensitivity

   Port-a-Patch Suction Control Pro pre-publication in bioRxiv (2018)

Authors:
Zhang M., Yao F., Pan C., Yan Z.

2018 - Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers

   Port-a-Patch and      Vesicle Prep Pro publication in Current Protocols in Pharmacology (2018)

Authors:
Klaerke D.A., de los Angeles Tejada M., Grøsfjeld Christensen V., Lassen M., Amstrup Pedersen P., Calloe K.

2018 - Quantifying Permeation of Small Charged Molecules across Channels: Electrophysiology in Small Volumes

   Port-a-Patch and      Vesicle Prep Pro publication in ACS Omega (2018)

Authors:
Wang J., Benier L., Winterhalter M.

2018 - Powering the ABC multidrug exporter LmrA: How nucleotides embrace the ion-motive force

   Port-a-Patch publication in Science Advances (2018)

Authors:
Agboh K., Lau C.H.F., Khoo Y.S.K., Singh H., Raturi S., Nair A.V., Howard J., Chiapello M., Feret R., Deery M.J., Murakami S., van Veen H.W.

2018 - Parallel evolution of frog antimicrobial peptides produces identical conformations but subtly distinct membrane and antibacterial activities

   Port-a-Patch and      Vesicle Prep Pro pre-publication in bioRxiv (2018)

Authors:
Manzo G., Ferguson P.M., Hind C., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton M.J., Lorenz C.D., Phoenix D.A., Mason A.J.

2018 - Non-blocking modulation contributes to sodium channel inhibition by a covalently attached photoreactive riluzole analog

   Port-a-Patch publication in Nature Scientific Reports (2018)

Authors:
Lukacs P., Mátyás F.C., Valánszki L., Casanova E., Biri-Kovács B., Nyitray L., Málnási-Csizmadia A., Mike A.

2018 - Molecular mechanism of the Orai channel activation

   Port-a-Patch and      Vesicle Prep Pro Pre-publicatin in bioRxiv (2018)

Authors:
Liu, X., Wu, G., Yu, Y., Chen, X., Ji, R., Lu, J., Li, X., Zhang, X., Yang, X., Shen, Y.

2018 - Interaction of Gravity with Cellular Compounds

   Port-a-Patch book chapter in Gravitational Biology II (2018)

Authors:
Hanke W., Kohn F.P.M., Neef M. Hampp R. 

2018 - Inhibition of pannexin-1 channel activity by adiponectin in podocytes: Role of acid ceramidase activation

   Port-a-Patch publication in BBA - Molecular and Cell Biology of Lipids (2018)

Authors:
Li G., Zhang Q., Hong J., Ritter J.K., Li P.-L.

2018 - Functional Analysis of Membrane Proteins Produced by Cell-Free Translation

   Port-a-Patch and      Orbit mini article in Protein Engineering (2018)

Authors:
Dondapati S.K., Wüstenhagen D.A., Kubick S.

2018 - Enhanced Cardiomyocyte NLRP3 Inflammasome Signaling Promotes Atrial Fibrillation

   Port-a-Patch publication in Circulation (2018)

Authors:
Yao C., Veleva T., Scott L., Cao S., Li L., Chen G., Jeyabal P., Pan X., Alsina K.M., Abu-Taha I., Ghezelbash S., Reynolds C.L., Shen Y.H., LeMaire S.A., Schmitz W., Müller F.U., El-Armouche A., Eissa N.T., Beeton C., Nattel S. , Wehrens X.H.T., Dobrev D., Li N.

2018 - Electrophysiological experiments in microgravity: lessons learned and future challenges

   Port-a-Patch publication in Microgravity (2018)

Authors:
Wuest S.L., Gantenbein B., Ille F.,Egli M.

2018 - An aromatic cluster in Lysinibacillus sphaericus BinB involved in toxicity and proper in-membrane folding

   Port-a-Patch and      Vesicle Prep Pro publication in Archives of Biochemistry and Biophysics (2018)

Authors:
Chooduang A., Surya W., Torres J., Boonserm P.

2018 - An allosteric mechanism of inactivation in the calcium-dependent chloride channel BEST1

   Port-a-Patch publication in Journal of General Physiology (2018)

Authors:
Vaisey G., Long S.B.

2018 - 3-Iodothyronamine Activates a Set of Membrane Proteins in Murine Hypothalamic Cell Lines

   Port-a-Patch publication in Frontiers in Endocrinology (2018)

Authors:
Bräunig J., Mergler S., Jyrch S., Hoefig C.S., Rosowski M., Mittag J., Biebermann H., Khajavi N.

2017 - The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports

Authors:
Pham T., Perry J.L., Dosey T.L., Delcour L.H.,Hyser J.M.

2017 - Synthetic Channel Specifically Inserts into the Lipid Bilayer of Gram-Positive Bacteria but not that of Mammalian Erythrocytes

   Port-a-Patch publication in Angewandte Chemie (2017)

Authors:
Zhang M., Zhu P.P., Xin P., Si W., Li Z.T., Hou J.L.

2017 - Prolonged immunomodulation in inflammatory arthritis using the selective Kv1.3 channel blocker HsTX1[R14A] and its PEGylated analog

   Port-a-Patch publication in Clinical Immunology (2017)

Authors:
Tanner M.R., Tajhya R.B., Huq R., Gehrmann E.J., Rodarte K.E., Atik M.A., Norton R.S., Pennington R.W., Beeton C.

2017 - Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

   Port-a-Patch and      Vesicle Prep Pro publication in PLoS ONE (2017)

Authors: 
Rangel M., Fernandes dos Santos Castro F., Mota-Lima L.D., Clissa P.B., Martins D.B., Perez dos Santos Cabrera M., Mortari M.R.

2017 - Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans

   Port-a-Patch publication in Journal of Venomous Animals and Toxins including Tropical Diseases (2017)

Authors:
Kazuma K., Ando K., Nihei K., Wang X., Rangel M., Franzolin M.R., Mori-Yasumoto K., Sekita S., Kadowaki M., Satake M., Konno K.

2017 - Organelle membrane derived patches: reshaping classical methods for new targets

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports (2017)

Authors:
Shapovalov G., Ritaine A., Bidaux G., Slomianny, C., Borowiec A-S., Gordienko D., Bultynck G., Skryma R., Prevarskaya N.

2017 - Mitochondria sustain store-operated currents in colon cancer cells but not in normal colonic cells: reversal by non-steroidal anti-inflammatory drugs

   Port-a-Patch publication in Oncotarget (2017)

Authors:
Hernández-Morales M., Sobradillo D., Valero R.A., Muñoz E., Ubierna D., Moyer M.P., Núñez L., Villalobos C.

2017 - Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)

   Port-a-Patch and      Vesicle Prep Pro publication in International Journal of Antimicrobial Agents (2017)

Authors: 
Silva J.C., Neto L.M., Neves R.C., Gonçalves J.C., Trentini M.M., Mucury-Filho R., Smidt K.S., Fensterseifer I.C., Silva O.N., Lima L.D., Clissa P.B., Vilela N., Guilhelmelli F., Silva L.P., Rangel M., Kipnis A., Silva-Pereira I., Franco O.L., Junqueira-Kipnis A.P., Bocca A.L., Mortari M.R.

2017 - Cytotoxic effect of zinc oxide nanoparticles on murine photoreceptor cells via potassium channel block and Na+/K+‐ATPase inhibition

   Port-a-Patch publication in Cell Proliferation (2017)

Authors:
Chen C. Bu W., Ding H., Li Q., Wang D., Bi H., Guo D.

2017 - Buserelin alleviates chloride transport defect in human cystic fibrosis nasal epithelial cells

  Port-a-Patch publication in PLoS ONE (2017)

Authors: 
Calvez M.L., Benz N., Huguet F., Saint-Pierre A., Rouillé E., Coraux C., Férec C., Kerbiriou M., Trouvé P.

2017 - Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel

  Port-a-Patch and      Vesicle Prep Pro publication in Structure (2017)

Authors: 
Arya P, Jarerattanachat V., Clausen M.V., Schewe M., McClenaghan C., Argent L., Conrad L.J., Dong Y.Y., Pike A.C.W., Carpenter E.P., Baukrowitz T., Sansom M.S.P., Tucker S.J.

2017 - Asymmetric mechanosensitivity in a eukaryotic ion channel

   Port-a-Patch and      Vesicle Prep Pro publication in PNAS (2017)

Authors:
Clausen M.V., Jarerattanachata V., Carpenterc E.P., Mark S. , Sansomb P., Tucker S.J.

2017 - A mutant of the Buthus martensii Karsch antitumor-analgesic peptide exhibits reduced inhibition to hNav1.4 and hNav1.5 channels while retaining analgesic activity

   Port-a-Patch publication in Journal of Biological Chemistry (2017)

Authors:
Xu Y., Meng X., Hou X., Sun J., Kong X., Sun Y., Liu Z., Ma Y., Niu Y., Song Y., Cui Y., Zhao M., Zhang J.

2016 - Voltage Dependence of Conformational Dynamics and Subconducting States of VDAC-1

  Port-a-Patch publication in Biophysical Journal (2016)

Authors: 
Briones R., Weichbrodt C., Paltrinieri L., Mey I., Villinger S., Giller K., Lange A., Zweckstetter M., Griesinger C., Becker S., Steinem C., de Groot B.L.

2016 - Upregulation of Transient Receptor Potential Vanilloid Type-1 Channel Activity and Ca2+ Influx Dysfunction in Human Pterygial Cells

  Port-a-Patch publication in Investigative Opthalmology & Visual Science (2016)

Authors: 
Garreis F., Schröder A., Reinach P.S., Zoll S., Khajavi N., Dhandapani P., Lucius A., Pleyer U., Paulsen F., Mergler S.

2016 - The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor

  Port-a-Patch and      Vesicle Prep Pro publication in Nature (2016)

Authors: 
Survery S., Moparthi L., Kjellbom P., Högestätt E.D., Zygmunt P.M., Johanson U.

2016 - Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola

  Port-a-Patch and      Vesicle Prep Pro publication in Nature Communications (2016)

Authors:
Dhakshnamoorthy B., Rohaim A., Rui H., Blachowicz L., Roux B.

2016 - Reversible control of current across lipid membranes by local heating

  Port-a-Patch publication in Scientific Reports (2016)

Authors: 
Urban P., Kirchner S.R., Mühlbauer C., Lohmüller T., Feldmann J.

2016 - Pore architecture of TRIC channels and insights into their gating mechanism

  Port-a-Patch and      Vesicle Prep Pro publication in Nature (2016)

Authors: 
Yang H., Hu M., Guo J., Ou X., Cai T., Liu Z.

2016 - Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1

  Port-a-Patch and      Vesicle Prep Pro publication in The Journal of Neuroscience (2016)

Authors: 
Babes A., Sauer S.K., Moparthi L., Kichko T.I., Neacsu C., Namer B., Filipovic M., Zygmunt P.M., Reeh P.W., Fischer M.J.

2016 - Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity

  Port-a-Patch and      Vesicle Prep Pro publication in Scientific Reports (2016)

Authors: 
Moparthi L., Kichko T. I., Eberhardt M., Högestätt E. D., Kjellbom P., Johanson U., Reeh P., W., Leffler A., Filipovic M. R., Zygmunt P. M.

2016 - Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system

  Port-a-Patch and      Vesicle Prep Pro publication in Protein Expression and Purification (2016)

Authors: 
Soranzo T., Cortès S., Gilde F., Kreir M., Picart C., Lenormand J.-L.

2016 - Distinct regions that control ion selectivity and calcium-dependent activation in the bestrophin ion channel

  Port-a-Patch publication in Proceedings of the National Academy of Sciences of the United States of America (2016)

Authors: 
Vaisey G., Miller A.N., Long S.B.

2016 - Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

  Port-a-Patch publication in Stem Cell Reviews and Reports (2016)

Authors: 
Lidgerwood G.E., Lim S.Y., Crombie D.E., Ali R., Gill K.P., Hernández D., Kie J., Conquest A., Waugh H.S., Wong R.C., Liang H.H., Hewitt A.W., Davidson K.C., Pébay A.

2016 - Coupling Data Mining and Laboratory Experiments to Discover Drug Interactions Causing QT Prolongation

  Patchliner publication in Journal of the American College of Cardiology (2016)

Authors:
Lorberbaum T., Sampson K.J., Chang J.B., Iyer V., Woosley R.L., Kass R.S., Tatonetti N.P.

2016 - Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biomedical Science and Engineering (2016)

Authors: 
Sieber M., Kaltenbach S., Hanke W. and Kohn F.P.M

2015 - The Functional Property Changes of Muscular Nav1.4 and Cardiac Nav1.5 Induced by Scorpion Toxin BmK AGP-SYPU1 Mutants Y42F and Y5F

  Port-a-Patch publication in Biochemistry (2015)

Authors: 
Meng x., Xu Y., Zhao M., Wang F., Ma Y., Jin Y., Liu Y., Song Y., Zhang J.

2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of the American Chemical Society (2015)

Authors: 
Cama J., Bajaj H., Pagliara S., Maier T., Braun Y., Winterhalter M., Keyser U.F.

2015 - K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac

  Port-a-Patch publication in Science (2015)

Authors: 
Dong Y.Y., Pike A.C., Mackenzie A., McClenaghan C., Aryal P., Dong L., Quigley A., Grieben M., Goubin S., Mukhopadhyay S., Ruda G.F., Clausen M.V., Cao L., Brennan P.E., Burgess-Brown N.A., Sansom M.S., Tucker S.J., Carpenter E.P.

2015 - Golgi anti-apoptotic proteins are highly conserved Ion Channels That Affect Apoptosis and Cell Migration

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

Authors: 
Carrara G., Saraiva N., Parsons M., Byrne B., Prole D.L., Taylor C.W., Smith G.L.

2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

Authors: 
Billen B., Brams M., Debaveye S., Remeeva A., Alpizar Y.A., Waelkens E., Kreir M., Brüggemann A., Talavera K., Nilius B., Voets T., Ulens C.

2015 - Cell-free expression of a functional pore-only sodium channel

  Port-a-Patch and      Vesicle Prep Pro publication in Protein Expression and Purification (2015)

Authors:
Kovácsová G, Gustavsson E, Wang J, Kreir M, Peuker S, Westenhoff S.

2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

  Orbit 16,      Port-a-Patch and      Vesicle Prep Pro publication in Analyst (2015)

Authors:
Weichbrodt C., Bajaj H., Baaken G., Wang J., Guinot S., Kreir M, Behrends J.C., Winterhalter M., Fertig N.

2014 - Ultra-stable glass microcraters for on-chip patch clamping

  Port-a-Patch publication in Joyal Society of Chemistry Advances (2014)

Authors: 
Stava E., Shin H.C.,Yu M., Bhat A., Resto P.J., Seshadri A., Williams J.C., Blick R.H.

2014 - Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

   Port-a-Patch and      Vesicle Prep Pro publication in Biomedical Optics Express (2014)

Authors:
Kniggendorf A.-K., Meinhardt-Wollweber M., Yuan X., Roth B., Seifert A., Fertig N., Zeilinger C.

2014 - Studying mechanosensitive ion channels with an automated patch clamp

  Port-a-Patch and      Vesicle Prep Pro publication in European Biophysics Journal (2014)

Authors:
Barthmes M.,  Mac Donald F.J., Birkner J.P., Brüggemann A., Wahl-Schott C., Koçer A.

2014 - Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biological Chemistry (2014)

Authors: 
Li Y., Surya W., Claudine S., Torres J.

2014 - Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

  Port-a-Patch and      Vesicle Prep Pro publication in Biosensors and Bioelectronics (2014)

Authors: 
Dondapati S.K., Kreir M., Quast R.B., Wüstenhagen D.A., Brüggemann A., Fertig N., Kubick S.

2014 - L-Carnitine Reduces in Human Conjunctival Epithelial Cells Hypertonic- Induced Shrinkage through Interacting with TRPV1 Channels

  Port-a-Patch publication in Cellular Physiology and Biochemistry (2014)

Authors:
Khajavi N., Reinach P.S., Skrzypski M., LudeA., Mergler S.

2014 - KCNMA1 Encoded Cardiac BK Channels Afford Protection against Ischemia-Reperfusion Injury

  Port-a-Patch publication in PLoS One (2014)

Authors: 
Soltysinska E., Bentzen B.H., Barthmes M., Hattel H., Thrush A.B., Harper M.E., Qvortrup K., Larsen F.J., Schiffer T.A., Losa-Reyna J., Straubinger J., Kniess A., Thomsen M.B., Brüggemann A., Fenske S., Biel M., Ruth P., Wahl-Schott C., Boushel R.C., Olesen S.P., Lukowski R.

2014 - Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells

  Port-a-Patch publication in PLoS One (2014)

Authors: 
Benz N., Le Hir S., Norez C., Kerbiriou M., Calvez M.-L., Becq F., Trouvé P. , Férec C.

2014 - Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain

  Port-a-Patch and      Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

Authors: 
Moparthi L., Survery S., Kreir M., Simonsen C., Kjellbom P., Högestätt E.D., Johanson U., Zygmunt P.M.

2014 - High susceptibility to fatty liver disease in two-pore channel 2-deficient mice

  Port-a-Patch publication in Nature Communications (2014)

Authors: 
Grimm C., Holdt L.M., Chen C.C., Hassan S., Müller C., Jörs S., Cuny H., Kissing S., Schröder B., Butz B., Northoff B., Castonguay J., Luber C.A., Moser M., Spahn S., Lüllmann-Rauch R., Fendel C., Klugbauer N., Griesbeck O., Haas A., Mann M., Bracher F., Teupser D., Saftig P., Biel M., Wahl-Schott C.

2014 - Crystal structure of a human GABAA receptor

  Port-a-Patch publication in Nature (2014)

Authors:
Miller P.S., Aricescu A.R.

2014 - Calcium regulation by temperature-sensitive transient receptor potential channels in human uveal melanoma cells

  Port-a-Patch publication in Cellular Signalling (2014)

Authors:
Mergler S., Derckx R., Reinach P.S., Garreis F., Böhm A., Schmelzer L., Skosyrski S., Ramesh N., Abdelmessih S., Polat O.K., Khajavi N., Riechardt A.I.

2014 - Automated Patch Clamp Analysis of nAChα7 and NaV1.7 Channels

  Port-a-Patch and      Patchliner publication in Current Protocols in Pharmacology (2014)

Authors: 
Obergrussberger A., Haarmann C., Rinke I., Becker N., Guinot D., Brueggemann A., Stoelzle-Feix S., George M., Fertig N.

2014 - An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity

  Port-a-Patch and      Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

Authors: 
Danilchanka A., Sun J., Pavlenok M., Maueröder C., Speer A., Siroy A., Marrero J., Trujillo C., Mayhew D.L., Doornbos K.S., Muñoz 'L.E., HerrmannM., EhrtS., Berens C., Niederweisa M.

2014 - A small molecule restores function to TRPML1 mutant isoforms responsible for mucolipidosis type IV

  Port-a-Patch publication in Nature Communications (2014)

Authors:
Chen C.C., Keller M., Hess M., Schiffmann R., Urban N., Wolfgardt A., Schaefer M., Bracher F., Biel M., Wahl-Schott C., Grimm C.

2014 - A potent and Kv1.3-selective analogue of the scorpion toxin HsTX1 as a potential therapeutic for autoimmune diseases

  Port-a-Patch publication in Nature Scientific Reports (2014)

Authors: 
Rashid M.H., Huq R., Tanner M.R., Chhabra S., Khoo K.K., Estrada R., Dhawan V., Chauhan S., Pennington M.W., Beeton C., Kuyucak S., Norton R.S.

2013 - Monoacylglycerols Activate TRPV1 – A Link between Phospholipase C and TRPV1

  Port-a-Patch publication in PLoS One (2013)

Authors: 
Zygmunt P.M., Ermund A., Movahed P., Andersson D.A., Simonsen C., Jönsson B.A., Blomgren A., Birnir B., Bevan S., Eschalier A., Mallet C., Gomis A., Högestätt E.D.

2013 - Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells

  Port-a-Patch publication in Experimental Eye Research (2013)

Authors: 
Mergler S., Mertens C., Valtink M., Reinach P.S., Castelo Székely V., Slavi N., Abdelmessih S., Türker E., Fels G., Pleyer U.

2013 - Differential Effects of the β‐Adrenoceptor Blockers Carvedilol and Metoprolol on SQT1‐ and SQT2‐Mutant Channels

  Port-a-Patch publication in Journal of Cardiovascular Electrophysiology (2013)

Authors: 
Bodi I., Franke G., Pantulu N.D., Wu K., Perez-Feliz S., Bode C., Zehender M., Zur Hausen A., Brunner M., Odening K.

2013 - Crystal structure and functional mechanism of a human antimicrobial membrane channel

  Port-a-Patch publication in Proceedings of the National Academy of Sciences of the United States of America (2013)

Authors: 
Songa C., Weichbrodt C., Salnikov E.S., Dynowski M.,Forsberg B.O., Bechinger B., Steinem C., de Groot B.L., Zachariae U. and Zeth K.

2013 - A Potent and Selective Peptide Blocker of the Kv1.3 Channel: Prediction from Free-Energy Simulations and Experimental Confirmation

   Port-a-Patch publication in PLoS ONE (2013)

Authors:
Rashid M.H., Heinzelmann G., Huq R., Tajhya R.B., Chang S.C., Chhabra S., Pennington M.W., Beeton C., Norton R.S., Kuyucak S.

2012 - Thermo-sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells

   Port-a-Patch publication in Cellular Signalling (2012)

Authors: 
Mergler S., Skrzypski M., Sassek M., Pietrzak P., Pucci C., Wiedenmann B., Strowski M.Z.

2012 - Selective modification of the N-Terminal structure of polytheonamide B significantly changes its cytotoxicity and activity as an ion channel

   Port-a-Patch publication in ChemMedChem (2012)

Authors: 
Shinohara N., Itoh H., Matsuoka S., Inoue M.

2012 - Regulation of Hyperpolarization-activated Cyclic Nucleotide-gated (HCN) Channel Activity by cCMP

   Port-a-Patch publication in Journal of Biological Chemistry (2012)

Authors: 
Zong X., Krause S., Chen C.C., Krüger J., Gruner C., Cao-Ehlker X., Fenske S., Wahl-Schott C., Biel M.

2012 - Natural and artificial ion channels for biosensing platforms

   Port-a-Patch,      Patchliner,      SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and      Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

Authors: 
Steller L., Kreir M., Salzer R.

2012 - Isolation, characterization and total regioselective synthesis of the novel μO-conotoxin MfVIA from Conus magnificus that targets voltage-gated sodium channels

   Port-a-Patch publication in Biochemical Pharmacology (2012)

Authors: 
Vetter I., Dekan Z., Knapp O., Adams D.J., Alewood P.F., Lewis R.J.

2012 - Interleukin-1R3 mediates interleukin-1–induced potassium current increase through fast activation of Akt kinase

   Port-a-Patch publication in Proceedings of the National Academy of Sciences of the Unites States of America (2012)

Authors: 
Qian J., Zhu L., Li Q., Belevych N., Chen Q., Zhao F., Herness S., Quan N.

2012 - Insulin-secreting INS-1E cells express functional TRPV1 channels

   Port-a-Patch publication in Islets (2012)

Authors: 
Fågelskiöld A.J., Kannisto K., Boström A., Hadrovic B., Farre C., Eweida M., Wester K., Islam MD.S.

2012 - HTS techniques for patch clamp-based ion channel screening - economy and advances

   Port-a-Patch,      Patchliner and      SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Expert Opinion on Drug Discovery (2012)

Authors: 
Farre C. and Fertig N.

2012 - Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayer

   Port-a-Patch and      Vesicle Prep Pro publication in The Journal of Biological Chemistry (2012)

Authors: 
Carnarius C., Kreir M., Krick M., Methfessel C., Moehrle V., Valerius O., Brüggemann A., Steinem C., Fertig N.

2012 - Effects of the Antitussive Drug Cloperastine on Ventricular Repolarization in Halothane-Anesthetized Guinea Pigs

   Port-a-Patch publication in Journal of Pharmacologigal Sciences (2012)

Authors: 
Takahara A., Fujiwara K., Ohtsuki A., Oka T., Namekata I., Tanaka H.

2012 - DNA unzipping and protein unfolding using nanopores

   Port-a-Patch publication in Methods of Molecular Biology (2012)

Authors: 
Merstorf C., Cressiot B., Pastoriza-Gallego M., Oukhaled A.G., Bacri L., Gierak J., Pelta J., Auvray L., Mathé J.

2012 - Design, synthesis and functional analysis of dansylated polytheonamide mimic: an artificial Peptide ion channel

   Port-a-Patch and      Vesicle Prep Pro publication in Journal of the American Chemical Society (2012)

Authors: 
Itoh H., Matsuoka S., Kreir M., Inoue M.

2012 - Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells

   Port-a-Patch publication in Histochemistry and Cell Biology (2012)

Authors: 
Mergler S., Garreis F., Sahlmüller M., Lyras E.-M., Reinach P.S., Dwarakanath A., Paulsen F., Pleyer U.

2012 - Alternative splicing of the N-terminal cytosolic and transmembrane domains of P2X7 controls gating of the ion channel by ADP-ribosylation

   Port-a-Patch publication in PLoS ONE (2012)

Authors: 
Schwarz N., Drouot L., Nicke A., Fliegert R., Boyer O., Guse A.H., Haag F., Adriouch S., Koch-Nolte F.

2011 - Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins

  Port-a-Patch and      Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States (2011)

Authors: 
Shaya D., Kreir M., Robbins R.A., Wong S., Hammon J., Brüggemann A., Minor Jr. D.L.

2011 - Thermosensitive transient receptor potential channels in human corneal epithelial cells

   Port-a-Patch publication in Journal of Cellular Physiology (2011)

Authors: 
Mergler S., Garreis F., Sahlmüller M., Reinach P.S., Paulsen F., and Pleyer U.

2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening

   Port-a-Patch,     Patchliner and      SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)

Authors: 
Stoelzle S., Obergrussberger A., Brüggemann A., Haarmann C., George M., Kettenhofen R., Fertig N.

2011 - Physiology of the Human Corneal Endothelium – New Insights from Electrophysiological Investigations

   Port-a-Patch publication in Klinische Monatsblätter für Augenheilkunde (2011)

Authors: 
Mergler S. and Pleyer U.

2011 - Functional Analysis of Synthetic Substructures of Polytheonamide B: A Transmembrane Channel-Forming Peptide

   Port-a-Patch publication in Angewandte Chemie International Edition (2011)

Authors: 
Matsuoka S., Shinohara N., Takahashi T., Iida M., Inoue M.

2011 - Chemical and biological characterization of four new linear cationic a-helical peptides from the venoms of two solitary eumenine wasps

   Port-a-Patch publication in Toxicon (2011)

Authors: 
Rangel M., dos Santos Cabrera M.P., Kazuma K., Ando K., Wang X., Kato M., Nihei K., HirataI.Y., Cross T.J., Garcia .N., Faquim-Mauro E.L., Franzolin M.R., Fuchino H., Mori-Yasumoto K., SekitaS., Kadowaki M., SatakeM., KonnoK.

2011 - Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

   Port-a-Patch publication in Experimental Eye Research (2011)

Authors: 
Mergler S., Valtink M., Taetz K., Sahlmüller M., Fels G., Reinach P.S., Engelmann K., Pleyer U.

2011 - Cardiac Toxicity

   Patchliner and      Port-a-Patch book chapter in ADMET for Medicinal Chemists: A Practical Guide (2011)

Authors: 
Kettenhofen R., Schwengberg S.

2011 - Bacterial mechanosensitive channels as a paradigm for mechanosensory transduction

   Port-a-Patch publication in Cellular Physiology and Biochemistry (2011)

Authors:
Martinac B.

2011 - Automated Patch Clamp on mESC-Derived Cardiomyocytes for Cardiotoxicity Prediction

   Patchliner and      Port-a-Patch publication in Journal of Biological Chemistry (2011)

Authors: 
Stoelzle S., Haythornthwaite A., Kettenhofen R., Kolossov E., Bohlen H., George M., Brüggemann A., Fertig N.

2010 - TRPV channels mediate temperature-sensing in human corneal endothelial cells

  Port-a-Patch publication in Experimental Eye Research (2010)

Authors: 
Mergler S., Valtink M., Coulson-Thomas V.J., Lindemann D., Reinach P.S., Engelmann K., Pleyer U.

2010 - Studying mechanosensitive ion channels using liposomes

  Port-a-Patch and      Vesicle Prep Pro publication in Methods in Molecular Biology (2010)

Authors: 
Martinac B., Rohde P.R., Battle A. R., Petrov E., Pal P, Alexander Foo A.FW, Vásquez V., Huynh T. and Kloda A.

2010 - Renaissance of ion channel research and drug discovery by patch clamp automation

  Port-a-Patch,      Patchliner and      SyncroPatch 96 (a predecessor model of SyncroPatch 384PE)  publication in Future Medical Chemistry (2010)

Authors:
Farre C. and Fertig N.

2010 - Planar Patch Clamp Approach to Characterize Ionic Currents from Intact Lysosomes

  Port-a-Patch publication in Science Signaling (2010)

Authors:
Schieder M., Rötzer K., Brüggemann A., Biel M., Wahl-Schott C.

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

Authors: 
Mahendran K.R., Kreir M., Weingart H., Fertig N., Winterhalter M.

2010 - Cor.At Cardiomyocytes: Primary-like Cardiomyocytes for Manual and Automated Electrophysiological Screening

  Port-a-Patch and      Patchliner publication in Lonza Resource Notes (2010)

Authors:
Kettenhofen R., Stölzle S.

2010 - Characterization of two pore channel 2 (TPCN2) -mediated Ca2+ currents in isolated lysosomes

  Port-a-Patch publication in The Journal of Biological Chemistry (2010)

Authors:
Schieder M., Rötzer K., Brüggemann A., Biel M., Wahl-Schott C.A.

2009 - Transient receptor potential ankyrin 1 antagonists block the noxious effects of toxic industrial isocyanates and tear gases

  Port-a-Patch publication in FASEB (2009)

Authors:
Bessac B.F., Sivula M., von Hehn C.A., Caceres A.I., Escalera, J, Jordt S.

2009 - The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Structural Biology (2009)

Authors:
Gaßmann O., Kreir M., Ambrosi C., Pranskevich J., Oshima A, Röling C., Sosinsky G., Fertig N., Steinem C.

2009 - T-type channel blocking properties and antiabsence activity of two imidazo[1,2-b]pyridazine derivatives structurally related to indomethacin

  Port-a-Patch publication in Neuropharmacology (2009)

Authors: 
Rimoli M.G., Russo E., Cataldi M., Citraro R., Ambrosino P., Melisi D., Curcio A., De Lucia S., Patrignani P., De Sarro G., Abignente E.

2009 - Port-a-Patch and Patchliner: High fidelity electrophysiology for secondary screening and safety pharmacology

  Port-a-Patch and      Patchliner publication in Combinatorial Chemistry & High Throughput Screening (2009)

Authors: 
Farre C., Haythornthwaite A., Haarmann C., Stoelzle S., Kreir M., George M., Brüggemann A., Fertig N.

2009 - Amyloid-beta-Induced Ion Flux in Artificial Lipid Bilayers and Neuronal Cells: Resolving a Controversy

  Port-a-Patch and      Vesicle Prep Pro publication in Neurotoxicity Research (2009)

Authors: 
Capone R., Quiroz F.G., Prangkio P., Saluja I., Sauer A.M., Bautista M.R., Turner R.S., Yang J., Mayer M.

2008 - TRPA1 is a major oxidant sensor in murine airway sensory neurons

  Port-a-Patch publication in The Journal of Clinical Investigation (2008)

Authors: 
Bessac B.F., Sivula M., von Hehn C.A., Escalera J., Cohn L., Jordt S.E.

2008 - Synthesis and biological evaluation of chalcones as inhibitors of the voltage-gated potassium channel Kv1.3

  Port-a-Patch publication in Bioorganic & Medicinal Chemistry Letters (2008)

Authors: 
Cianci J., Baell J.B., Flynn B.L., Gable R.W., Mould J.A., Paul D., Harvey A.J.

2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes

  Port-a-Patch and      Vesicle Prep Pro publication in Lab-on-a-chip (2008)

Authors: 
Kreir M., Farre C., Beckler M., George M., Fertig N.

2008 - Planar patch-clamp force microscopy on living cells

  Port-a-Patch publication in Ultramicroscopy (2008)

Authors: 
Pamir E., George M., Fertig N., Benoit M.

2008 - Planar patch clamp: Advances in electrophysiology

  Port-a-Patch book chapter in "Potassium Channels" (2008)

Authors: 
Brüggemann A., Farre C., Haarmann C., Haythornthwaite A., Kreir M., Stoelzle S., George M., Fertig N.

2008 - Ion channel screening – automated patch clamp on the rise

  Port-a-Patch and      Patchliner publication in Drug Discovery Today (2008)

Authors: 
Farre C., George M., Brüggemann A., Fertig N.

2008 - High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology

  Port-a-Patch publication in Nature Reviews Drug Discovery (2008)

Authors: 
Dunlop J., Bowlby M., Peri R., Vasilyev D., Arias R.

2007 - Planar Patch Clamping

  Port-a-Patch and      Patchliner book chapter in "Patch Clamp Analysis – Advanced Techniques", Series: Neuromethods (2007)

Authors: 
Behrends, J.C., Fertig, N.

2007 - Electrophysiological assessment of HERG blockade: Comparative study using automated and conventional patch clamp systems

  Port-a-Patch publication in Toxicological Letters (2007)

Authors: 
Yook Y., Kim J., Cho H., Moon H., Kwak B.

2007 - Automated ion channel screening: patch clamping made easy

  Port-a-Patch and      Patchliner publication in Expert Opinion Therapeutic Targets (2007)

Authors: 
Farre C., Stoelzle S., Haarman C., George M., Brueggemann A., Fertig N.

2006 - Microchip technology for automated and parallel patch clamp recording

  Port-a-Patch and      Patchliner publication in Small Journal (2006)

Authors: 
Brüggemann A., Stoelzle S., George M., Behrends J.C., Fertig N.

2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation

  Port-a-Patch and      Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)

Authors: 
Sondermann M., George M., Fertig N., Behrends J.C.

2005 - The Port-a-Patch: The smallest patch clamp set up for high quality electrophysiology

  Port-a-Patch publication in HEKA Impulse (2005)

Authors: 
Brüggemann A., George M., Klau M., Beckler M., Steindl J., Behrends J., Fertig N.

2005 - Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles

  Port-a-Patch publication in Nano Letters (2005)

Authors: 
Kirchner C., Liedl T., Kudera S., Pellegrino T., Munoz J. A., Gaub H.E., Stoelzle S., Fertig N., Parak W.J.

2004 - Ion channel drug discovery and research: The automated Nano-Patch-Clamp technology

  Port-a-Patch publication in Current Drug Discovery Technologies (2004)

Authors: 
Brüggemann A., George M., Klau M., Beckler M., Steindl J., Behrends J.C., Fertig N.

2003 - Simultaneous optical and electrical recording of single gramicidin channels

  Port-a-Patch publication in Biophysical Journal (2003)

Authors: 
Borisenko V., Lougheed L., Hesse J., Fuereder-Kitzmueller E., Fertig N., Behrends, J.C., Woolley G.A., Schuetz G.J.

2003 - Microstructured apertures in planar glass substrates for ion channel research

  Port-a-Patch publication in Channels and Receptors (2003)

Authors: 
Fertig N., Meyer C., Tilke A., George M., Klau M., Sobotta C., Blick R.H., Behrends J.C.

2003 - Lighting up single ion channels

  Port-a-Patch publication in Biophysical Journal (2003)

Authors: 
Selvin P.R.

2003 - High quality ion channel analysis on a chip with the NPC-technology

  Port-a-Patch publication in Assay and Drug Development Technologies (2003)

Authors: 
Brüggemann A., George M., Klau M., Beckler M., Steindl J., Behrends J.C., Fertig N.

2002 - Whole cell patch clamp recording performed on a planar glass chip

  Port-a-Patch publication in Biophysical Journal (2002)

Authors: 
Fertig N., Blick R.H., Behrends J.C

2002 - Patch clamp on a chip

  Port-a-Patch publication in Biophysical Journal (2002)

Authors: 
Sigworth F.J., Klemic K.G.

2002 - Activity of single ion channel proteins detected with a planar microstructure

  Port-a-Patch publication in Applied Physics Letters (2002)

Authors: 
Fertig N., Klau M., George M., Blick R.H., Behrends J.C.

2001 - Microstructured glass chip for ion channel electrophysiology

  Port-a-Patch publication in Physical review. E, Statistical, nonlinear, and soft matter physics (2001)

Authors: 
Fertig N., Meyer C., Blick R.H., Trautmann C., Behrends J.C.

2000 - Stable integration of isolated cell membrane patches in a nanomachined aperture

  Port-a-Patch publication in Applied Physics Letters (2000)

Authors: 
Fertig N., Tilke A., Blick R.H., Kotthaus J.P., Behrends J.C., ten Bruggencate G.

2015 - Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp

  SURFE²R N1 and     SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and     Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015      (1.6 MB)

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2015 - The backstage pass to study your favorite TRP channel

  Port-a-Patch and     Patchliner and SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and     SyncroPatch 384PE poster, TRP Meeting 2015      (2.2 MB)

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2018 - Combining electrophysiology and contractility recordings for more complete assessment of hiPSC-CMs

   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i),      Patchliner and      CardioExcyte 96 poster, Europhysiology Meeting 2018     (1.4 MB)

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2018 - Expression and pharmacology of GluA2-containing AMPA receptors in cell lines and stem cell-derived neurons

   Port-a-Patch,      Patchliner and      SyncroPatch 384PE  (a predecessor model of the SyncroPatch 384i) poster, Europhysiology Meeting 2018     (0.9 MB)

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