• Port-a-Patch

    世界上最小的膜片钳系统
  • Port-a-Patch

    易学易用——教学的理想工具
  • Port-a-Patch

    可记录细胞、细胞器与脂双层
  • Port-a-Patch

    世界上首款平板芯片膜片钳设备
  • Port-a-Patch

    可进行内灌流——更换细胞内液的理想工具

Mitochondria - "Mitoplasts from HEK cells on Nanion‘s Port-a-Patch"

icon pap   Port-a-Patch application note   logo pdf   (0.5 MB)

Summary:

Mitochondria play an important role in metabolism by providing the cell with ATP due to oxidative phosphorylation. But they are also supposed to be involved in apoptosis and cytoprotection. The mitochondrial membranes contain a large number of ion channels, transporters and pores. However the physiological role of mitochondrial channels is largely unknown. The main channel of the outer membrane is VDAC, a well described anion conductance. In the inner membrane most prominent are the permeability transition pore (PTP) and the inner membrane anion channel IMAC. Different potassium channels are described as well (mitoBK, mitoKATP and Kv1.3) and a number of calcium conducting channels and receptors (for example MCU). To study the channels of the inner mitochondrial membrane the outer membrane can be stripped of by osmotic swelling. This method was used for the examples of electrophysiological measurement with the Port-a-Patch shown below. 

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