• Port-a-Patch

    世界上最小的膜片钳系统
  • Port-a-Patch

    易学易用——教学的理想工具
  • Port-a-Patch

    可记录细胞、细胞器与脂双层
  • Port-a-Patch

    世界上首款平板芯片膜片钳设备
  • Port-a-Patch

    可进行内灌流——更换细胞内液的理想工具

KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:  logo pdf   (0.2 MB)

Summary:

Solvent-free planar lipid bilayers were formed in an automated manner using suction to attract a giant unilamellar vesicle (GUV) to the patch clamp chip which subsequently bursts across the aperture. Incubation of GUV's with purified KcsA channel protein yielded proteoliposomes. These proteoliposomes allow for immediate recording of channel activity after GUV sealing. The rapid formation of protein-containing planar lipid bilayers is of potential use for the efficient electrophysiological characterization of KcsA as shown here and also other ion channel proteins of interest.

返回总览

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.