SURFE²R 96SE - 高通量转运体研究设备
与 SURFE²R N1一样，SURFE²R 96SE基于固体支撑膜（SSM）电生理技术，解决转运体与离子泵电流过小的问题，同时将通量提高到平行进行96个记录。只需要将转运体样品加到96孔记录板镀金电极上。并且为了进一步减少用户的工作量，所有电极预处理步骤都由SURFE²R 96SE自动完成。并且可以加任何底物都可以加入激活转运体，且会记录相应的转运或绑定电流。所有的以上步骤，从制备样品、测定到数据分析都是自动进行的。
• 每天可获取10,000 个数据点
• 整合 CyBio® Felix 小型移液平台
• 拥有8个放置部同溶液的位点 (支持1孔板到96孔板)
• 已在超过100 目标上通过验证，发表了超过 100篇同行评审过的文献
• 每孔仅需要0.1 – 1 µg蛋白样品，并足够进行最多100次实验
• 实时数据与5 ms的高时间分辨率，非单点读取
• 可完成EC50、 IC50、转运速度常数、转运体亚型比较...
SURFE²R 96SE 设备与软件
The SURFE2R 96SE 设备
The SURFE2R (surface electrogenic event reader) technology is the only available commercial solution for SSM-based electrophysiology on the market. Nanion Technologies developed the SURFE2R N1 as an easy-to-learn all-in-one device for universities and basic research and the high-throughput device SURFE2R 96SE.
The SURFE2R 96SE is integrated into the CyBio® Felix liquid handling platform. Nanion Technologies provides the electrophysiological hardware and the computer running a software package for data recording and analysis. The device can not only be used for electrophysiological measurements, but also for sample preparation and pipetting purposes. The device can measure 10,000 data points a day in a fully automated manner. In contrast to the SURFE2R N1, the SURFE2R 96SE also comes with a dedicated data analysis software which allows for automated analysis of the massive amount of data you generate each day.
The SURFE2R 96SE uses sensors in a standard 96-well plate format. Membranes containing the protein of interest are adsorbed onto these sensors. The measurement is initiated by a substrate concentration jump. The solution exchange is controlled by the liquid handling platform CyBio® Felix. The pipetting head contains 96 pipettes which load solutions from solution positions and inject them to the sensor plate. All 96 sensors of the sensor plate are measured simultaneously, but different solutions or transporters can be used for each measurement.
The sensor plate is enclosed by a Faraday cage and connected to a 96-channel amplifier as well as 96 gold pins which work as reference electrodes. The CyBio® Felix holds up to 8 well plates for 8 to 8 x 96 different solutions which allows up to 7 sequential measurements using different conditions in each automated workflow. The CyBio® pipettor allows the measurement of fast kinetics with low solution consumption, showing a time resolution of down to 5 ms by using only 50 µl of compound solution per measurement.
Our software department developed a software package exclusively for the SURFE2R 96SE.
• The SurfControl works as the recording software and displays the data in real-time
• DataControl works as the analysis software and can be configured to automate your data analysis
• In addition, the CyBio Composer plus pre-installed workflows operate the liquid handling platform and
communicates with the recording software
SURFE²R 96SE 芯片板
The SURFE²R 96SE sensor plate is a proprietary innovative product by Nanion Technologies, developed for the SURFE²R 96SE. It is produced by an external partner, quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers.
MaterialThe core structure of SURFE²R 96SE sensor plates are gold-coated sensors with 3 mm diameter. This structure is incorporated into a 96 well plate for easy handling. The sensor plate is enclosed by a Faraday cage and connected to a 96-channel amplifier during the experiment.
Available plate type
- "SURFE²R 96SE Sensor Plate": 96-well recording plate for the SURFE²R 96SE (Order # 182001)