SURFE²R N1 - 转运体工作站

Dr. Arnaud Javelle - Statement about the SURFE²R N1 Device

  “Using the SURFE2R N1 we have recently obtained high quality data in a very short period of time. We have developed an assay to measure the activity of ammonium transporters from the Amt protein family. There has been considerable controversy over the mechanism of ammonium transport by Amt proteins and the controversy was due to the lack of quantitative kinetic data characterizing the activity of the proteins at the single channel level. The SSM technologies allows to overcome this hurdle and we are now capable of answering very challenging functional questions concerning the mechanisms and the energetics of these transporters."

Dr. Arnaud Javelle, Chancellor's fellow, Strathclyde Institute of Pharmacy and Biomedical Sciences

Dr. Thomas Seeger & Karin V. Niessen - Statement about the SURFE²R Technology

   “The SURFE²R makes extremely challenging electrophysiological targets accessible to robust routine analyses. For example, we have developed an assay for investigating nicotinic acetylcholine receptor ion channels, using membranes from the Pacific electric ray, Torpedo California. In this way, we overcome many of the well-known difficulties associated with this ion channel, which allows us to efficiently obtain information regarding compound pharmacology. Obtained pharmacology values match those of patch clamp recordings exceptionally well. We find that the SURFE²R is an excellent platform for characterization of electrogenic processes in isolated membranes.”

Karin V. Niessen, Dr. Thomas Seeger
Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany

Nathan Thomas - Statement about the SURFE²R N1 Device

  “We started collaborating with Nanion after receiving the Nanion´s SURFE²R N1 grant in 2018. The SURFE²R N1 has generated terrific functional data from the very first day it was installed in our lab. In general, it's very reliable and easy to use. Also, whenever we have run into issues or had questions about results, Nanion has provided prompt and helpful support”

Nathan Thomas
5th Year PhD student at University of Wisconsin-Madison, USA (Dr. Henzler-Wildman's lab)

Prof. Dr. Klaus Fendler - Statement about the SURFE²R N1 Device

  “SSM-based electrophysiology is a well-established technique developed in our lab in the early 1990s for the investigation of electrogenic membrane transporters. It works with a wide range of samples from mammalian cell membranes over microbial membrane vesicles to proteoliposomes. SSM-based electrophysiology is especially useful in cases where conventional electrophysiology cannot be applied, e.g. for transporters residing in intracellular membranes or plasma membranes of small microbial cells. The SURFE²R N1 is a commercial instrument for SSM based electrophysiology which facilitates basic research in the field of membrane transport.“

Prof. Dr. Klaus Fendler
Max-Planck Institute of Biophysics, Frankfurt, Germany

Prof. Dr. Klaus Fendler and Co-Authors - Interview about the SURFE²R Technology

   ““SSM-based electrophysiology is a suitable technique to study bacterial ion-translocating membrane- systems which are otherwise difficult to approach using conventional electrophysiological techniques, like patch clamp.
Additionally, the mechanical stability of SSMs facilitates the transport activation through fast solution exchange, a condition that is technically challenging in a setup of a bilayer separating two solution-containing compartments.“

Dr. Miyer Fabián Patiño-Ruiz,
Faculty of Science and Engineering, University of Groningen, the Netherlands

Dr. Andre Bazzone, Application Scientist for the SURFE²R product family interviewed the authors of the publication:
"Mutation of two key aspartate residues alters stoichiometry of the NhaB Na⁺/H⁺ exchanger from Klebsiella pneumoniae"
Published 2019 in Nature Scientific Reports.

Read the publication here and download the full case study here:

   SURFE²R Technology Customer Case Study (2020)      (1.3 MB)
Nature Scientific Reports: Characterization of the Na⁺/H⁺ exchanger NhaB on the SURFE²R N1

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Prof. Dr. Randy Stockbridge - Interview about the SURFE²R Technology

   “We wanted to characterize the electrophysiological characteristics of our new family of transporters to find out properties such as K_m and V_max of the substrate, and ideally to screen a set of substrates for transporter activity. However, given our difficulties in developing a transport assay, I was pessimistic that we were going to be able to move beyond radiolabeled substrates.
Contrary to my expectations, electrophysiology with the SURFE²R N1 platform worked right away. We saw very robust currents in our pilot experiments during the initial training session on the instrument.“

Prof. Dr. Randy Stockbridge, Assistant Professor
University of Michigan, USA

Download the full case study here:

   SURFE²R Technology Customer Case Study (2019)      (1.8 MB)
Replacing Radiolabeling Techniques with the SURFE²R N1

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Adenine Nucleotide Translocator (ANT) - Recorded on the Surfer

  SURFE²R N1 data and applications:

The Adenine Nucleotide Translocator (ANT) is located in the inner membrane of mitochondria and transports, as the name suggests, either ADP or ATP alone or against each other.  Recordings were done on mitochondria inner membrane preparations from pigs heart.  Example traces display the action of ANT either in the presence of ATP, in an other experiment in the presence of ADP.

AmtB - Activation by ammonium

 SURFE²R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE²R N1. Shown are transient currents measured after a 100 mM ammonium jump in empty liposomes (green) or proteoliposomes containing AmtB at a lipid protein ratio (LPR) of 50 (black), 10 (red) or 5 (blue). Insert: Normalized current measured in proteoliposomes containing AmtB at an LPR of 50 (black), 10 (red) or 5 (blue).

Data from Mirandela et al, 2018

AmtB - Substrate specificity

 SURFE²R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE²R N1. AmtB substrate specificity. (A) Transient current measured after a 100 mM substrate jump. Ammonium (red), methylammonium (black), potassium (green) or sodium (purple). Potassium and sodium do not act as substrates for AmtB and methylammonium translocates but at a much reduced rate compared with ammonium. Insert: Normalised current after a 100 mM substrate jump. Ammonium (red), methylammonium (black).

Data from Mirandela et al, 2018

Clc - EC50 for inward and outward directed chloride flux

  SURFE²R N1 data and applications:

We determined EC50 and relative Vmax for Cl- in an Cl-/H+ exchange assay for both transport directions and found a slight asymmetry in transporter kinetics.

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Clc - Inhibition with OADS

  SURFE²R N1 data and applications:

We investigated the effect of the specific Clc inhibitor OADS on the transport activity of Clc and its reversibility.

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Clc - Inward and outward directed chloride flux

  SURFE²R N1 data and applications:

According to the transport direction of 2Cl^-/1H⁺ exchange, we observed positive (Cl^- efflux) or negative (Cl^- influx) transient currents.

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Clc - Proteoliposomes with different lipid-to-protein ratios

  SURFE²R N1 data and applications:

We tested proteoliposomes with different densities of reconstituted Clc protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.

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EAAT3 - Current Traces at different Sodium Chloride Concentrations

   SURFE²R N1 data and applications:

EAAT3 current traces were recorded after application of different Sodium Chloride concentrations. The EC₅₀ of the peak current was determined as 42.2 mM. 

NCX_Mj - EC50 for Calcium and Magnesium

  SURFE²R N1 data and applications:

Measurement of the transport activity of NCX_Mj. The first solution exchange provides a Na⁺-free solution to establish an outward directed Na⁺ gradient; the second solution exchange provides the divalent cation to activate the ion exchanger. By applying different concentrations of one divalent cation on the same sensor, we determined EC₅₀ for Ca²⁺, Mg²⁺ and Sr²⁺.

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NCX1 - Block by KB-R7943

   SURFE²R N1 data and applications:

Cardiac NCX1 expressed in iCell^® Cardiomyocytes², Cor.4U^® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.   

NCX1 - Block by SEA0400

   SURFE²R N1 data and applications:

Cardiac NCX1 expressed in iCell^® Cardiomyocytes² or HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  

NCX1 - Current traces recorded from Cor.4U Cardiomyocytes, iCell Cardiomyocytes and HEK cells

   SURFE²R N1 data and applications:

Cardiac NCX1 was activated using 30 μM Ca²⁺ on the SURFE²R N1. NCX1 was recorded from Cor.4U^® Cardiomyocytes, iCell^® Cardiomyocytes² or overexpressed in HEK cells. The calcium affinity was determined for NCX1 recorded from iCell^® Cardiomyocytes² and HEK cells, shown on the right.  

Organic Cation Transporter 2 (OCT2) - Pharmacology

 SURFE²R N1 data and applications:

EC₅₀ values and relative V_max for three different substrates are shown.

The IC₅₀ value for Choline was determined as 4.6 mM, for TEA as 0.33 mM and for Metformin as 8.6 mM.

PepT1 - Pharmacology of Lys[Z(NO2)]-Val

  SURFE²R N1 data and applications:

Representive traces of PepT1 overexpressed in CHO cells. PepT1 (SLC15A1) is expressed mainly in intestine and kidney, it enables uptake of oligopeptides. The PepT1 signal is evoked by glycylglycin. The transport of oligopeptides can be inhibited by addition of Lys[Z(NO2)]-Val. The IC₅₀ of Lys[Z(NO2)]-Val was 380µM.

Respiratory Chain Complex I and III - Current Over Mitochondrial Membrane

  SURFE²R N1 data and applications:

In the respiratory chain Complex I and III, oxidation of NADH leads to transport of 4 protons across the membrane. The electrons are transferred to cytochrome c. Because of the electron leakage to oxygen, both complex are the main sites of production of harmful superoxide. In these experiments using the SURFE²R N1, application of 100µM NADH results in a current over the mitochondrial membrane, where Complex I and III are expressed.

Respiratory Chain Complex II and III - Current Traces

  SURFE²R N1 data and applications:

Respiratory Chain Complex II is a succinate dehydrogenase. In this complex, succinate is oxidated to fumarate. The electrons are transfered to the quinone pool, the Complex III (cytochrome bc₁ complex) and cytochrome c, while four protons are transported to the intermembrane space.

Respiratory Chain Complex IV - Current Traces

  SURFE²R N1 data and applications:

The Respiratory Chain Complex IV, known as Cytochrom c oxidase, reduces oxygen by cunsumption of electrons from cytocrome c and transport of two protons to the intermembrans space. Here the reaction runs backwards.

Respiratory Chain Complex V - Action of the ATP Synthase after Application of ATP

  SURFE²R N1 data and applications:

The Respiratory Chain Complex V uses the transmembrane proton gradient (produced by Complex I, III and IV) to generates ATP from ADP plus phosphate. One component of ATP synthase acts as an ion channel that provides for a proton flux back into the mitochondrial matrix. This reflux releases free energy, which is used to drive ATP synthesis, catalyzed by the other component of the complex. Experiments on the SURFE²R N1 show the action of the ATPase after application of ATP.

SERCA - IC50 for Cisplatin

  SURFE²R N1 data and applications:

Ca²⁺-transport in SERCA is triggered by ATP hydrolysis. Using ATP concentration jumps, the authors determined the IC₅₀ of SERCA inhibition by cisplatin

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SGLT1 - Current Traces after Application of alpha-MDG

   SURFE²R N1 data and applications:

Current traces after application of 10 mM and 50 mM alpha-MDG.

Sodium-Potassium ATPase - Analysis

  SURFE²R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na⁺/K⁺ pump is responsible for the active transport of Na⁺ and K⁺ in the cells containing relatively high concentrations of K⁺ ions but low concentrations of Na⁺ ions. The Na⁺/K⁺-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na⁺ and K⁺, or in the presence of ATP and Na⁺.

2020 - SURFE²R N1 Product Video

  SURFE²R N1 product video

Label free, real time measurements - electrophysiological approach. Record the action of billion transporters simultaneously.

2018 - Transporters Investigated Using the SURFE²R Instruments

   SURFE²R 96SE and      SURFE²R N1 Oral Presentation

Presenter: 
Dr. Maria Barthmes, Product Manager SURFE²R product family, Nanion Technologies GmbH, Germany

2018 - Electrophysiological Characterization of Sugar Transporters using SSM-based Electrophysiology

   SURFE²R 96SE and      SURFE²R N1 Oral Presentation

Presenter: 
Dr. Andre Bazzone, Application Scientist, Nanion Technologies GmbH, Germany
Andre is an expert in the field of SSM-based electrophysiology: He made his PhD at the Max-Planck-Institute of Biophysics in Frankfurt, Germany on the electrophysiological characterization of sugar transporters using the SSM-based electrophysiology in 2016. Right afterwards, he started as Application Scientist at Nanion Technologies and today he is an important member of the Nanion SURFE²R team.

2017 - Variations on the Coupling Theme in Secondary Active Transport

   SURFE²R N1 Oral Presentation

Presenter: 
Gregor Madej, University of Regensburg, Germany

CNT1 - "Electrophysiological recordings of CNT1 (SLC28A1) activity on Nanion’s SURFE²R N1"

  SURFE²R N1 application note      (0.4 MB)

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EAAT3 - "Electrophysiological recordings of EAAT3 (SLC1A1) activity on Nanion’s SURFE²R N1"

  SURFE²R application note     (0.4 MB)

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GAT1 - "Electrophysiological recordings of hGAT1 (SLC6A1) activity on Nanion’s SURFE²R N1"

  SURFE²R application note     (0.4 MB)

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Neurons - "Electrophysiological recordings of LGIC and AA transporters in iCell® GlutaNeurons"

   Patchliner,     SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) and      SURFE²R N1 application note:      (0.5 MB)
Cells were kindly provided by FUJIFILM Cellular Dynamics, Inc.  

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PepT1 - "Electrophysiological recordings of PepT1 (SLC15A1) activity on Nanion’s SURFE²R"

   SURFE²R N1 application note:      (0.9 MB)

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SURFE2R N1 - 产品单页

  SURFE²R N1 产品单页      (1.0 MB)

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SURFE²R N1 - Quickstart Guide "Protocols"

   SURFE²R N1 Quickstart Guide "Protocols"

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2020 - Unlocking the Reversal Potential of Solid Supported Membrane Electrophysiology to Determine Transport Stoichiometry

   SURFE²R N1 pre-publication in bioRxiv (2020)

Authors:
Henzler-Wildman K.A., Thomas N.E.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level (2)

  SURFE²R 96SE and       SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors: 
Tadini-Buoninsegni F.,Palchetti I.

2020 - Energy Coupling in Cation-Pumping Pyrophosphatase—Back to Mitchell

   SURFE²R N1 publication in Frontiers in Plant Science (2020)

Authors:
Baykov A.A.

2019 - Phosphatidylserine flipping by the P4-ATPase ATP8A2 is electrogenic

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PNAS (2019)

Authors:
Tadini-Buoninsegni F., Mikkelsen S.A., Mogensen L.S., Molday R.S., and Andersen J.P.

2019 - Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

   SURFE²R N1 publication in Nature Scientific Reports (2019)

Authors:
Patiño-Ruiz M., Fendler K., & Călinescu O.

2019 - Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

   SURFE²R N1 and      Orbit 16 publication in Frontiers in Pharmacology (2019)

Authors:
Dondapati S.K., Lübberding H., Zemella A., Thoring L., Wüstenhagen D.A., Kubick S.

2019 - A two-lane mechanism for selective biological ammonium transport

   SURFE²R N1 pre-publication in bioRxiv (2019)

Authors:
Williamson G., Tamburrino G., Mirandela G.D., Boeckstaens M., Bage M., Pisliakov A., Ives C.M., Terras E.T., Bizior A., Hoskisson P.A., Marini A.M., Zachariae U., Javelle A.

2018 - The lipid environment determines the activity of the E. coli ammonium transporter, AmtB

   SURFE²R N1 publication in Faseb J. (2018)

Authors:
Mirandela G.D., Tamburrino G., Hoskisson P.A., Zachariae U., Javelle A.

2017 - SSM-Based Electrophysiology for Transporter Research

  SURFE²R N1 and     SURFE²R 96SE book chapter in Methody in Enzymology

Authors:
Bazzone A., Barthmes M., Fendler K.

2017 - Mechanisms of charge transfer in human copper ATPases ATP7A and ATP7B

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in IUBMB Life (2017)

Authors:
Tadini-Buoninsegni F., Smeazzetto S.

2017 - Insights into the mechanism of membrane pyrophosphatases by combining experiment and computer simulation

  SURFE²R N1 publication in Structural Dynamics (2017)

Authors: 
Shah N.R., Wilkinson C., Harborne S.P.D., Turku A., Li K.-M., Sun Y.-J., Harris S., Goldman A.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Discovery of Compounds that Positively Modulate the High Affinity Choline Transporter

  SURFE²R N1 publication in Frontiers in Molecular Neuroscience (2017)

Authors: 
Choudhary P., Armstrong E.J., Jorgensen C.C., Piotrowski M., Barthmes M., Torella R., Johnston S.E., Maruyama Y., Janiszewski J.S., Storer R.I., Skerratt S.E., Benn C.L.

2017 - Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2017)

Authors:
Smeazzetto S., Armanious G.P., Moncelli M.R., Bak J.J., Lemieux M.J., Young H.S., Tadini-Buoninsegni F.

2017 - Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

  SURFE²R-technology (custom-built system)  publication in PLoS ONE (2017)

Authors:
Patiño-Ruiz M., Ganea C., Fendler K., Călinescu O.

2016 - pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases

  SURFE²R-technology (custom-built system) publication in PLoS ONE (2016)

Authors:
Bazzone A., Madej M.G., Kaback H.R., Fendler K.

2016 - Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism

  SURFE²R N1 publication in Nature Communications (2016)

Authors: 
Li K., Wilkinson C., Kellosalo J., Tsai J., Kajander T, Jeuken L.J.C., Sun Y., Goldman A.

2016 - Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems

   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2016)

Authors:
Srinivasan L., Baars T.L., Fendler K., Michel H.

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

  SURFE²R N1 and      Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2016 - Electrogenic Cation Binding in the Electroneutral Na+/H+ Antiporter of Pyrococcus abyssi

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2016)

Authors:
Călinescu O., Linder M., Wöhlert D., Yildiz Ö., Kühlbrandt W., Fendler K.

2016 - Charge translocation by mitochondrial NADH:ubiquinone oxidoreductase (complex I) from Yarrowia lipolytica measured on solid-supported membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical and Biophysical Research Communications (2016)

Authors:
Siebels I., Dröse S.

2015 - Structural and Functional Studies of NirC from Salmonella typhimurium

   SURFE²R-technology (custom-built system) publication in Methods in Enzymology (2015)

Authors:
Rycovska-Blume A., Lü W., Andrade S., Fendler K., Einsle O.

2015 - Hofmeister effect of anions on calcium translocation by sarcoplasmic reticulum Ca2+-ATPase

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Nature Scientific Reports (2015)

Authors:
Tadini-Buoninsegni F., Moncelli M.R., Peruzzi N., Ninham B.W., Dei L., Lo Nostroa P.

2015 - A universal mechanism for transport and regulation of CPA sodium proton exchangers

  SURFE²R-technology (custom-built system) publication in Biological Chemistry (2015)

Authors:
Călinescu O., Fendler K.

2015 - A sulfur‐based transport pathway in Cu+‐ATPases

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in EMBO Reports (2015)

Authors:
Mattle D., Zhang L., Sitsel O., Pedersen L.T., Moncelli M.R., Tadini-Buoninsegni F., Gourdon P., Rees D.C., Nissen P., Meloni G.

2014 - Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling

  SURFE²R-technology (custom-built system) publication in Nature Communications (2014)

Authors:
Perez C., Faust B., Mehdipour A.R., Francesconi K.A., Forrest L.R., Ziegler C.

2014 - Species differences in bacterial NhaA Na+/H+ exchangers

  SURFE²R-technology (custom-built system) publication in FEBS Letters (2014)

Authors:
Călinescu O., Danner E., Böhm M., Hunte C., Fendler K..

2014 - NhaA Na+/H+ antiporter mutants that hardly react to the membrane potential

  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Alkoby D., Rimon A., Budak M., Patino-Ruiz M., Călinescu O., Fendler K., Padan E..

2014 - Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium

  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Lentes C.J., Mir S.H., Boehm M., Ganea C., Fendler K., Hunte C.

2014 - Keeping it simple, transport mechanism and pH regulation in Na+/H+ exchangers

   SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2014)

Authors:
Călinescu O., Paulino C., Kühlbrandt W., Fendler K.

2014 - Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch 96 Using Instant Frozen Cells

    SURFE²R N96 (predecessor model of SURFE²R 96SE) book chapter in Patch-Clamp Methods and Protocols

Authors:
Polonchuk L.

2014 - Direct observation of electrogenic NH4(+) transport in ammonium transport (Amt) proteins

   SURFE²R-technology (custom-built system) publication in PNAS (2014)

Authors:
Wacker T., Garcia-Celma J.J., Lewe P., Andrade S.L.

2014 - Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP-Dependent Ca2+ Translocation by Sarco-Endoplasmic Reticulum Ca2+-ATPase (SERCA)

  SURFE²R N1 publication in ChemMedChem (2014)

Authors: 
Sadafi F.Z., Massai L., Bartolommei G., Moncelli M.R., Messori L., Tadini-Buoninsegni F.

2013 - Photocycle and vectorial proton transfer in a rhodopsin from the eukaryote Oxyrrhis marina

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2013)

Authors:
Janke C., Scholz F., Becker-Baldus J., Glaubitz C., Wood P.G., Bamberg E., Wachtveitl J., Bamann C.

2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

   SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)

Authors:
Garcia-Celma J., Szydelko A., Dutzler R.

2013 - Enhanced adsorption of Ca-ATPase containing vesicles on a negatively charged solid supported membrane for the investigation of membrane transporters

  SURFE²R N1 publication in Langmuir (2013)

Authors: 
Sacconi A., Moncelli M.R., Mergheri G., Tadini-Buoninsegni F.

2013 - Electrophysiological characterization of uncoupled mutants of LacY

  SURFE²R-technology (custom-built system) publication in Biochemistry (2013)

Authors:
Gaiko O., Bazzone A., Fendler K., Kaback H.R.

2013 - Differential Effects of Mutations on the Transport Properties of the Na+/H+ Antiporter NhaA from Escherichia coli*

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2013)

Authors:
Mager T., Braner M., Kubsch B., Hatahet L., Alkoby D., Rimon A., Padan E., Fendler K.

2012 - The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter

   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2012)

Authors:
Rycovska A., Hatahet L., Fendler K., Michel H.

2012 - Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP

  SURFE²R-technology (custom-built system) publication in PNAS (2012)

Authors:
Khafizov K., Perez C., Koshy C., Quick M., Fendler K., Ziegler C., Forrest L.R.

2012 - Distinctive features of catalytic and transport mechanisms in mammalian sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) and Cu+ (ATP7A/B) ATPases

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2012)

Authors:
Lewis D., Pilankatta R., Inesi G., Bartolommei G., Moncelli M.R., Tadini-Buoninsegni F.

2012 - Assaying the proton transport and regulation of UCP1 using solid supported membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in European Biophysics Journal (2012)

Authors:
Blesneac I., Ravaud S., Machillot P., Zoonens M., Masscheylen S., Miroux B., Vivaudou M., Pebay-Peyroula E.

2011 - Transport Mechanism and pH Regulation of the Na+/H+ Antiporter NhaA from Escherichia coli

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2011)

Authors:
Mager T., Rimon A., Padan E., Fendler K.

2011 - Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

  SURFE²R ONE (a predecessor model of the SURFE²R N1) publication in Australian Journal of Chemistry (2011)

Authors:
Gaiko O., Janausch I., Geibel S., Vollert H., Arndt P., Gonski S., Fendler K.

2011 - G117C MelB, a mutant melibiose permease with a changed conformational equilibrium

  SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Ganea C., Meyer-Lipp K., Lemonnier R., Krah A., Leblanc G., Fendler K.

2011 - Development of an assay for Complex I/Complex III of the respiratory chain using solid supported membranes and its application in mitochondrial toxicity screening in drug discovery

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2011)

Authors:
Preissl S., Bick I., Obrdlik P., Diekert K., Gul S., Gribbon P.

2011 - Characterisation of the purified human sodium/iodide symporter reveals that the protein is mainly present in a dimeric form and permits the detailed study of a native C-terminal fragment

   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Huc-Brandt S., Marcellin D., Graslin F., Averseng O., Bellanger L., Hivin P., Quemeneur E., Basquin C., Navarro V., Pourcher T., Darrouzet E.

2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)

Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.

2010 - Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Pflügers Archiv - European Journal of Physiology (2010)

Authors:
Balannik V., Obrdlik P., Inayat S., Steensen C., Wang J., Rausch J.M., DeGrado W.F., Kelety B., Pinto L.H.

2010 - Electrophysiology of respiratory chain complexes and the ADP-ATP exchanger in native mitochondrial membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2010)

Authors:
Watzke N., Diekert K., Obrdlik P.

2010 - Electrophysiological characterization of ATPases in native synaptic vesicles and synaptic plasma membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical Journal (2010)

Authors:
Obrdlik P., Diekert K., Watzke N., Keipert C., Pehl U., Brosch C., Boehm N., Bick I., Ruitenberg M., Volknandt W., Kelety B.

2010 - Delineating electrogenic reactions during lactose/H+ symport

  SURFE²R-technology (custom-built system) publication in Biochemistry (2010)

Authors:
Garcia-Celma J.J., Ploch J., Smirnova I., Kaback H.R., Fendler K.

2010 - ATP dependent charge movement in ATP7B Cu+-ATPase is demonstrated by pre-steady state electrical measurements

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2010)

Authors:
Tadini-Buoninsegni F., Bartolommei G., Moncelli M.R., Pilankatta R., Lewis D., Inesi G.

2009 - Measuring Ion Channels on Solid Supported Membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2009)

Authors:
Schulz P., Dueck B., Mourot A., Hatahet L., Fendler K.

2009 - Inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Chemical Research in Toxicology (2009)

Authors:
Gramigni E., Tadini-Buoninsegni F., Bartolommei G., Santini G., Chelazzi G., Moncelli M.R.

2009 - Electrophysiological characterization of LacY

  SURFE²R-technology (custom-built system) publication in PNAS (2009)

Authors:
Garcia-Celma J.J., Smirnova I.N., Kaback H.R., Fendler K.

2009 - Electrogenic ion pumps investigated on a solid supported membrane: comparison of current and voltage measurements

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Langmuir (2009)

Authors:
Bartolommei G., Moncelli M.R., Rispoli G., Kelety B., Tadini-Buoninsegni F.

2009 - DtpB (YhiP) and DtpA (TppB, YdgR) are prototypical proton-dependent peptide transporters of Escherichia coli

  SURFE²R ONE (a predecessor model of SURFE²R N1) publication in The FEBS Journal (2009)

Authors:
Harder D., Stolz J., Casagrande F., Obrdlik P., Weitz D., Fotiadis D., Daniel H.

2009 - An automatic electrophysiological assay for the neuronal glutamate transporter mEAAC1

   SURFE²R-technology (custom-built system) publication in Journal of Neuroscience Methods (2009)

Authors:
Krause R., Watzke N., Kelety B., Dörner W., Fendler K.

2008 - Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane

  SURFE²R-technology (custom-built system) publication in Langmuir (2008)

Authors:
Garcia-Celma J.J., Dueck B., Stein M., Schlueter M., Meyer-Lipp K., Leblanc G., Fendler K.

2008 - Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2008)

Authors:
Wimmer F., Oberwinkler T., Bisle B., Tittor J., Oesterhelt D.

2008 - Effect of Clotrimazole on the Pump Cycle of the Na,K-ATPase

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2008)

Authors:
Bartolommei G., Devaux N., Tadini-Buoninsegni F., Moncelli M., Apell H.-J.

2007 - Functional and structural characterization of a prokaryotic peptide transporter with features similar to mammalian PEPT1

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2007)

Authors:
Weitz D., Harder D., Casagrande F., Fotiadis D., Obrdlik P., Kelety B., Daniel H.

2006 - Transporter assays using solid supported membranes: a novel screening platform for drug discovery

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2006)

Authors:
Kelety B., Diekert K., Tobien J., Watzke N., Dörner W., Obrdlik P., Fendler K.

2006 - The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2006)

Authors:
Meyer-Lipp K., Séry N., Ganea C., Basquin C., Fendler K., Leblanc G.

2006 - Structure and function of prokaryotic glutamate transporters from Escherichia coli and Pyrococcus horikoshii

   SURFE²R-technology (custom-built system) publication in Biochemistry (2006)

Authors:
Raunser S., Appel M., Ganea C., Geldmacher-Kaufer U., Fendler K., Kühlbrandt W.

2006 - Establishment of Cell-Free Electrophysiology for Ion Transporters: Application for Pharmacological Profiling

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biomolecular Screening (2006)

Authors:
Geibel S., Flores-Herr N., Licher T., Vollert H.

2005 - Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli

   SURFE²R 500 (a predecessor model of SURFE²R N1) publication in Biochimica et Biophysica Acta (BBA) - Bioenergetics (2005)

Authors:
Zuber D., Krause R., Venturi M., Padan E., Bamberg E., Fendler K.

2004 - Time-resolved charge translocation by sarcoplasmic reticulum Ca-ATPase measured on a solid supported membrane

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2004)

Authors:
Tadini Buoninsegni F., Bartolommei G., Moncelli M.R., Inesi G., Guidelli R.

2004 - Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli

   SURFE²R-technology (custom-built system) publication in Journal of Molecular Biology (2004)

Authors:
Zhou A., Wozniak A., Meyer-Lipp K., Nietschke M., Jung H., Fendler K.

2004 - Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli

   SURFE²R-technology (custom-built system) publication in Journal of Molecular Biology (2004)

Authors:
Zhou A., Wozniak A., Meyer-Lipp K., Nietschke M., Jung H., Fendler K.

2004 - Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane

   SURFE²R-technology (custom-built system) publication in Bioelectrochemistry (2004)

Authors:
Bartolommei G., Buoninsegni F.T., Moncelli M.R.

2003 - Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2003)

Authors:
Burzik C., Kaim G., Dimroth P., Bamberg E., Fendler K.

2002 - Photocurrents Generated by Bacteriorhodopsin Adsorbed on Thiol/Lipid Bilayers Supported by Mercury

   SURFE²R-technology (custom-built system) publication in Langmuir (2002)

Authors:
Dolfi A., Tadini-Buoninsegni F., Moncelli M.R., Guidelli R.

2001 - Evidence for intraprotein charge transfer during the transport activity of the melibiose permease from Escherichia coli

  SURFE²R-technology (custom-built system) publication in Biochemistry (2001)

Authors:
Ganea C., Pourcher T., Leblanc G., Fendler K.

1999 - Kinetics of electrogenic transport by the ADP/ATP carrier

  SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Gropp T., Brustovetsky N., Klingenberg M., Müller V., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na/K-ATPase Investigated on Solid Supported Membranes: Rapid Solution Exchange with a New Technique

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K.

1993 - Charge transport by ion translocating membrane proteins on solid supported membranes

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1993)

Authors:
Seifert K., Fendler K., Bamberg E.

2020 - A novel approach to detect electrogenic transporter activity in intact cells applied to investigate iPSC derived cardiomyocytes and neurons

    SURFE²R N1 and      SURFE²R 96SE poster, 64^th Annual Meeting of the Biophysical Society      (1.6 MB)

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2018 - Label-free analysis of Na+/Ca2+- exchanger (NCX) isolated from iPSC-derived cardiomyocytes

   SURFE²R N1 and      SURFE²R 96SE poster, Europhysiology Meeting 2018     (1.5 MB)

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2018 - Transported by light: optogenetic control of NCX1

   SURFE²R N1 poster, Biophysics Annual Meeting 2018     (2.2 MB)

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2015 - Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp

  SURFE²R N1 and     SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and     Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015      (1.6 MB)

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2017 - An emerging technique for the characterization of transport proteins: SSM-based electrophysiology

  SURFE²R N1 and      SURFE²R SE96 poster, 19th IUPAB / 11th EBSA congress 2017     (3.3 MB)

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2015 - Functional Characterization of Prokaryotic NCX by Solid Supported Membrane Technology

  SURFE²R N1 poster, Gordon Research Conference 2015     (0.9 MB)

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2020 - Unlocking the (Reversal) Potential of SSM Electrophysiology: Transporter Stoichiometry with the SURFE²R N1

   SURFE²R N1 presentation (slide deck)    (1.7 MB)

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