• SURFE²R N1

    易学易用的集成设备,适用于教学与大学研究
  • SURFE²R N1

    可实现非标记的转运体功能试验
  • SURFE²R N1

    相对与膜片钳更高的信号放大: 转运 & 结合试验
  • SURFE²R N1

    市面上唯一的基于SSM电生理技术的设备
  • SURFE²R N1

    到手可用的高效转运体蛋白分析设备

SURFE²R N1 - 转运体工作站

与传统膜片钳设备相比,SURFE²R (表面电荷事件读取器,surface electrogenic event reader)是专用于测量带电转运体 (同向转运体、交换体与单向转运体)与离子泵的技术。 通常这些蛋白相对与离子通道的转运率较低。 SURFE²R技术通过更大的芯片面积从而同时记录最多达109 个转运体从而获取最佳的信噪比。SURFE²R N1是适用于基础研究与大学教学的设备。如果需要高通量, SURFE²R 96SE 可提供最多平行96芯片的转运体记录。

SURFE²R N1的特点

• 支持最多52种不同溶液的自动记录
• 每天可记录150数据点
• 移液设备、电生理设备与电脑整合的一体化设备
• 易学易用,适用于教学与科研

基于SSM的电生理技术特点

• 已在超过100 目标上通过验证,发表了超过 100篇同行评审过的文献
• 可测量转运体、离子泵与配体门控通道
• 也可测量电中性交换体、糖结合蛋白
• 可使用从细胞提纯的生物膜与囊泡或蛋白脂质体
• 非标记电学测量手段
• 每孔仅需要0.1 – 1 µg蛋白样品,并足够进行最多100次实验
• 实时数据与5 ms的高时间分辨率,非单点读取
• 相对膜片钳更高信号放大率
• 可完成快速绑定动力学测量
• 可完成EC50、 IC50、转运速度常数、转运体亚型比较...

基于固体支撑膜技术(solid-supported membrane,SSM) 的电生理技术建立于90年代末,关于SSM电生理技术的详细资料请参考 技术细节。 可重复使用的芯片包含一个镀金电极,在上面可以通过一次快速移液形成SSM,然后将包含转运体的样品加到SSM表面上。任何包含目标蛋白的膜制成品都可以用来测量,包括细胞破坏后形成的膜囊泡或重建提纯的蛋白形成的蛋白脂质体。这些样品可以冷冻保存数月甚至数年,因此不需要细胞培养室。
基于SSM的电生理技术的关键是通过溶液交换来施加底物或者配体来激活转运体,然后电荷的迁移可以被检测出来并被分析以得出需要的数据。 由于SSM的高稳定性,同一个样品可以进行上百个实验,所以可以测定诸如EC50 或IC50等参数 。拥有目前最先进的溶液交换的时间分辨率,SURFE²R N1不但可以测量慢的转运过程,也可以分析快速的结合反应以及确定其反应速度常数。 

SURFE²R N1易学易用,可以在一天时间内学会并掌握。该设备通过机械臂自动进行实验,极大的减少了操作人员的工作量。SURFE²R N1在一个设备里包含了实验所需的所有设备,包括溶液处理元件、电生理硬件和电脑主机。

 

更多信息:

SURFE²R N1设备与软件

The SURFE2R N1 设备

N1 slide 1

The SURFE2R (surface electrogenic event reader) technology is the only available commercial solution for SSM-based electrophysiology on the market. Nanion Technologies developed the SURFE2R 96SE, a high-throughput system mainly used in pharmaceutical industry for drug screening purposes and the SURFE2R N1 designed for basic research.

The SURFE2R N1 device contains electrophysiological hardware, liquid handling components and the computer running Windows, plus the data recording and analysis software SurfControl. It’s an easy-to-learn, all-in-one robotic workstation which can measure 150 data points a day in a fully automated manner.

The SURFE2R N1 works with reusable sensor chips on which the membranes containing the protein of interest are adsorbed. The measurement is initiated by a substrate concentration jump. The solution exchange is controlled by the Ionjet, a robot-controlled pipette which loads solution from storage containers and injects them to the sensor enclosed by a Faraday cage. The Ionjet allows the measurement of fast kinetics with low solution consumption. An autosampler on top of the device allows placing up to 53 solutions for automated sequential measurements. During the measurement, the transport current can be viewed, compared and analyzed within the SurfControl software. The main characteristics of the device include a low-noise amplifier and a large sensor surface which both ensure a superior signal to noise ratio.


SURFE2R N1 控制软件与自动化

The device comes with the SURFE2R N1 Control software pre-installed on the internal computer. This forms both the recording and analysis software. The functions of SurfControl include the managing and coding of workflows which represent protocols for SURFE2R assays. Workflows can contain multiple measurements, e.g. using different substrate concentrations or the comparison of transport before and after inhibition. The workflow includes parameters like duration, speed and volume of solution flow during the experiment, the number and sequence of different buffers, the number of repetitions per measurement, the incubation times between experiments and the volume used for rinsing the sensor after an experiment. During the run of one workflow no interference by the researcher is required.

Beside the design of the experiment itself, SurfControl enables the researcher to view and compare recorded traces. The graphical window allows peak detection and the calculation of the peak integrals including baseline subtraction. An additional results window is used for automated documentation of the traces including file name, values for peaks and integrals and the time of measurement. For further analysis, e.g. the fitting of kinetic parameters or the subtraction of negative control currents, the software is capable of exporting the data to the standard file format ASCII.

耗材

SURFE²R N1单通道记录芯片

N1 Round V2 400x237

The SURFE²R N1 sensor chip is a proprietary innovative product by Nanion Technologies, developed for the SURFE²R N1. It is produced by an external partner, quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers.


材质
The core structure of SURFE²R N1 sensor chip is a gold-coated sensor with 3 mm diameter. This structure is incorporated into a screw cap for easy handling. The SURFE²R N1 single sensor chips can be reused after an appropriate cleaning protocol.
可用的型号
  • "SURFE²R N1 Single Sensor Chip" (Order # 161001)

已经验证的对象: 基于SSM的电生理



用户评价与案例分析

Dr. Arnaud Javelle - Statement about the SURFE²R N1 Device

Icon N1  “Using the SURFE2R N1 we have recently obtained high quality data in a very short period of time. We have developed an assay to measure the activity of ammonium transporters from the Amt protein family. There has been considerable controversy over the mechanism of ammonium transport by Amt proteins and the controversy was due to the lack of quantitative kinetic data characterizing the activity of the proteins at the single channel level. The SSM technologies allows to overcome this hurdle and we are now capable of answering very challenging functional questions concerning the mechanisms and the energetics of these transporters."

Dr. Arnaud Javelle, Chancellor's fellow, Strathclyde Institute of Pharmacy and Biomedical Sciences

Dr. Thomas Seeger & Karin V. Niessen - Statement about the SURFE²R Technology

Icon N1   “The SURFE²R makes extremely challenging electrophysiological targets accessible to robust routine analyses. For example, we have developed an assay for investigating nicotinic acetylcholine receptor ion channels, using membranes from the Pacific electric ray, Torpedo California. In this way, we overcome many of the well-known difficulties associated with this ion channel, which allows us to efficiently obtain information regarding compound pharmacology. Obtained pharmacology values match those of patch clamp recordings exceptionally well. We find that the SURFE²R is an excellent platform for characterization of electrogenic processes in isolated membranes.”

Karin V. Niessen, Dr. Thomas Seeger
Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany

Nathan Thomas - Statement about the SURFE²R N1 Device

Icon N1  “We started collaborating with Nanion after receiving the Nanion´s SURFE²R N1 grant in 2018. The SURFE²R N1 has generated terrific functional data from the very first day it was installed in our lab. In general, it's very reliable and easy to use. Also, whenever we have run into issues or had questions about results, Nanion has provided prompt and helpful support”

Nathan Thomas
5th Year PhD student at University of Wisconsin-Madison, USA (Dr. Henzler-Wildman's lab)

Prof. Dr. Klaus Fendler - Statement about the SURFE²R N1 Device

Icon N1   “SSM-based electrophysiology is a well-established technique developed in our lab in the early 1990s for the investigation of electrogenic membrane transporters. It works with a wide range of samples from mammalian cell membranes over microbial membrane vesicles to proteoliposomes. SSM-based electrophysiology is especially useful in cases where conventional electrophysiology cannot be applied, e.g. for transporters residing in intracellular membranes or plasma membranes of small microbial cells. The SURFE2R N1 is a commercial instrument for SSM based electrophysiology which facilitates basic research in the field of membrane transport.“

Prof. Dr. Klaus Fendler
Max-Planck Institute of Biophysics, Frankfurt, Germany

Prof. Dr. Klaus Fendler and Co-Authors - Interview about the SURFE²R Technology

Icon N1   ““SSM-based electrophysiology is a suitable technique to study bacterial ion-translocating membrane- systems which are otherwise difficult to approach using conventional electrophysiological techniques, like patch clamp.
Additionally, the mechanical stability of SSMs facilitates the transport activation through fast solution exchange, a condition that is technically challenging in a setup of a bilayer separating two solution-containing compartments.“

Dr. Miyer Fabián Patiño-Ruiz,
Faculty of Science and Engineering, University of Groningen, the Netherlands


Dr. Andre Bazzone, Application Scientist for the SURFE²R product family interviewed the authors of the publication:
"Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae"
Published 2019 in Nature Scientific Reports.

Read the publication here and download the full case study here:

Icon N1   SURFE²R Technology Customer Case Study (2020)   logo pdf   (1.3 MB)
Nature Scientific Reports: Characterization of the Na+/H+ exchanger NhaB on the SURFE²R N1

返回总览

Prof. Dr. Randy Stockbridge - Interview about the SURFE²R Technology

Icon N1   “We wanted to characterize the electrophysiological characteristics of our new family of transporters to find out properties such as Km and Vmax of the substrate, and ideally to screen a set of substrates for transporter activity. However, given our difficulties in developing a transport assay, I was pessimistic that we were going to be able to move beyond radiolabeled substrates.
Contrary to my expectations, electrophysiology with the SURFE²R N1 platform worked right away. We saw very robust currents in our pilot experiments during the initial training session on the instrument.“

Prof. Dr. Randy Stockbridge, Assistant Professor
University of Michigan, USA

Download the full case study here:

Icon N1   SURFE²R Technology Customer Case Study (2019)   logo pdf   (1.8 MB)
Replacing Radiolabeling Techniques with the SURFE²R N1

返回总览

数据与文献

Adenine Nucleotide Translocator (ANT) - Recorded on the Surfer

Icon N1   Surfer ANT traceSURFE2R N1 data and applications:

The Adenine Nucleotide Translocator (ANT) is located in the inner membrane of mitochondria and transports, as the name suggests, either ADP or ATP alone or against each other.  Recordings were done on mitochondria inner membrane preparations from pigs heart.  Example traces display the action of ANT either in the presence of ATP, in an other experiment in the presence of ADP.

AmtB - Activation by ammonium

AmtB activation by ammonium

Icon N1 SURFE2R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE2R N1. Shown are transient currents measured after a 100 mM ammonium jump in empty liposomes (green) or proteoliposomes containing AmtB at a lipid protein ratio (LPR) of 50 (black), 10 (red) or 5 (blue). Insert: Normalized current measured in proteoliposomes containing AmtB at an LPR of 50 (black), 10 (red) or 5 (blue).

Data from Mirandela et al, 2018

AmtB - Substrate specificity

AmtB specificity for ammonium

Icon N1 SURFE2R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE2R N1. AmtB substrate specificity. (A) Transient current measured after a 100 mM substrate jump. Ammonium (red), methylammonium (black), potassium (green) or sodium (purple). Potassium and sodium do not act as substrates for AmtB and methylammonium translocates but at a much reduced rate compared with ammonium. Insert: Normalised current after a 100 mM substrate jump. Ammonium (red), methylammonium (black).

Data from Mirandela et al, 2018

ClC - EC50 for inward and outward directed chloride flux

Clc Figure 2   EC50 Efflux vs InfluxIcon N1   SURFE2R N1 data and applications:

We determined EC50 and relative Vmax for Cl- in an Cl-/H+ exchange assay for both transport directions and found a slight asymmetry in transporter kinetics.

Read more

ClC - Inhibition with OADS

Clc Figure 3   OADS inhibitionIcon N1   SURFE2R N1 data and applications:

We investigated the effect of the specific ClC inhibitor OADS on the transport activity of ClC and its reversibility.



Read more

ClC - Inward and outward directed chloride flux

Clc Figure 1   transient currents Influx vs EffluxIcon N1   SURFE2R N1 data and applications:

According to the transport direction of 2Cl-/1H+ exchange, we observed positive (Cl- efflux) or negative (Cl- influx) transient currents.



Read more

ClC - Proteoliposomes with different lipid-to-protein ratios

Clc Figure 4   LPRsIcon N1   SURFE2R N1 data and applications:

We tested proteoliposomes with different densities of reconstituted ClC protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.

Read more

EAAT3 - Current Traces at different Sodium Chloride Concentrations

EAAC1 SURFE2R N1 Website

Icon N1   SURFE2R N1 data and applications:

EAAT3 current traces were recorded after application of different Sodium Chloride concentrations. The EC50 of the peak current was determined as 42.2 mM. 

 

NCX_Mj - EC50 for Calcium and Magnesium

F1 2Icon N1   SURFE2R N1 data and applications:

Measurement of the transport activity of NCX_Mj. The first solution exchange provides a Na+-free solution to establish an outward directed Na+ gradient; the second solution exchange provides the divalent cation to activate the ion exchanger. By applying different concentrations of one divalent cation on the same sensor, we determined EC50 for Ca2+, Mg2+ and Sr2+.

Read more

NCX1 - Block by KB-R7943

NCX1 KBR7943 iCell Cor4U HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytes2, Cor.4U® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.   

 

 

NCX1 - Block by SEA0400

NCX1 iCell HEK SEA0400

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytesor HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  

 

NCX1 - Current traces recorded from Cor.4U Cardiomyocytes, iCell Cardiomyocytes and HEK cells

NCX1 Cor4U icell HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 was activated using 30 μM Ca2+ on the SURFE2R N1. NCX1 was recorded from Cor.4U® Cardiomyocytes, iCell® Cardiomyocytes2 or overexpressed in HEK cells. The calcium affinity was determined for NCX1 recorded from iCell® Cardiomyocytes2 and HEK cells, shown on the right.  

 

Organic Cation Transporter 2 (OCT2) - Pharmacology

OCT2 SURFE2R N1

Icon N1 SURFE2R N1 data and applications:

EC50 values and relative Vmax for three different substrates are shown.

The IC50 value for Choline was determined as 4.6 mM, for TEA as 0.33 mM and for Metformin as 8.6 mM.

PepT1 - Pharmacology of Lys[Z(NO2)]-Val

Surfer PepT1 traces IC50Icon N1   SURFE2R N1 data and applications:

Representive traces of PepT1 overexpressed in CHO cells. PepT1 (SLC15A1) is expressed mainly in intestine and kidney, it enables uptake of oligopeptides. The PepT1 signal is evoked by glycylglycin. The transport of oligopeptides can be inhibited by addition of Lys[Z(NO2)]-Val. The IC50 of Lys[Z(NO2)]-Val was 380µM.

Respiratory Chain Complex I and III - Current Over Mitochondrial Membrane

Surfer RC complexI III traceIcon N1   SURFE2R N1 data and applications:

In the respiratory chain Complex I and III, oxidation of NADH leads to transport of 4 protons across the membrane. The electrons are transferred to cytochrome c. Because of the electron leakage to oxygen, both complex are the main sites of production of harmful superoxide. In these experiments using the SURFE2R N1, application of 100µM NADH results in a current over the mitochondrial membrane, where Complex I and III are expressed.

Respiratory Chain Complex II and III - Current Traces

Surfer RC II IIIIcon N1   SURFE2R N1 data and applications:

Respiratory Chain Complex II is a succinate dehydrogenase. In this complex, succinate is oxidated to fumarate. The electrons are transfered to the quinone pool, the Complex III (cytochrome bc1 complex) and cytochrome c, while four protons are transported to the intermembrane space.

Respiratory Chain Complex IV - Current Traces

Surfer RC IVIcon N1   SURFE2R N1 data and applications:

The Respiratory Chain Complex IV, known as Cytochrom c oxidase, reduces oxygen by cunsumption of electrons from cytocrome c and transport of two protons to the intermembrans space. Here the reaction runs backwards.

 

 

Respiratory Chain Complex V - Action of the ATP Synthase after Application of ATP

Surfer RC VIcon N1   SURFE2R N1 data and applications:

The Respiratory Chain Complex V uses the transmembrane proton gradient (produced by Complex I, III and IV) to generates ATP from ADP plus phosphate. One component of ATP synthase acts as an ion channel that provides for a proton flux back into the mitochondrial matrix. This reflux releases free energy, which is used to drive ATP synthesis, catalyzed by the other component of the complex. Experiments on the SURFE2R N1 show the action of the ATPase after application of ATP.

SERCA - IC50 for Cisplatin

SERCA Fig 1 partIcon N1   SURFE2R N1 data and applications:

Ca2+-transport in SERCA is triggered by ATP hydrolysis. Using ATP concentration jumps, the authors determined the IC50 of SERCA inhibition by cisplatin

Read more

 

 

SGLT1 - Current Traces after Application of alpha-MDG

SGLT1 SURFER N1

Icon N1   SURFE2R N1 data and applications:

Current traces after application of 10 mM and 50 mM alpha-MDG.

 

Sodium-Potassium ATPase - Analysis

Surfer NaK ATPaseIcon N1   SURFE2R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na+/K+ pump is responsible for the active transport of Na+ and K+ in the cells containing relatively high concentrations of K+ ions but low concentrations of Na+ ions. The Na+/K+-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na+ and K+, or in the presence of ATP and Na+.

网络研讨会与影像

13.10.2020 | Webinar: Employing SSM electrophysiology to capture electrogenic fluxes in membrane proteins

Icon N1  SURFE2R N1 Webinar

Date: October 13. 2020

201012 Blog Image VUM 2020

Speakers:

Dr. Matthias Quick (Columbia University; NY, USA)


This is an on-demand webinar from Nan]i[on and Friends 2020.

2020 - SURFE²R N1 Product Video

Icon N1  SURFE²R N1 product video

Label free, real time measurements - electrophysiological approach. Record the action of billion transporters simultaneously.

2018 - Transporters Investigated Using the SURFE²R Instruments

Icon 96SE   SURFE²R 96SE and   Icon N1   SURFE²R N1 Oral Presentation

Presenter: 
Dr. Maria Barthmes, Product Manager SURFE²R product family, Nanion Technologies GmbH, Germany

2018 - Electrophysiological Characterization of Sugar Transporters using SSM-based Electrophysiology

Icon 96SE   SURFE²R 96SE and   Icon N1   SURFE²R N1 Oral Presentation

Presenter: 
Dr. Andre Bazzone, Application Scientist, Nanion Technologies GmbH, Germany
Andre is an expert in the field of SSM-based electrophysiology: He made his PhD at the Max-Planck-Institute of Biophysics in Frankfurt, Germany on the electrophysiological characterization of sugar transporters using the SSM-based electrophysiology in 2016. Right afterwards, he started as Application Scientist at Nanion Technologies and today he is an important member of the Nanion SURFE²R team.

2017 - Variations on the Coupling Theme in Secondary Active Transport

Icon N1   SURFE²R N1 Oral Presentation

Presenter: 
Gregor Madej, University of Regensburg, Germany

下载:

应用数据

CIC - "Electrophysiological recordings of the H+/ Cl- exchanger Ec-ClC on the SURFE2R N1"

Icon N1   SURFE2R N1 application note:   logo pdf   (0.3 MB)
Samples kindly provided by Dr. Merritt Maduke, Stanford University, USA 

CNT1 - "Electrophysiological recordings of CNT1 (SLC28A1) activity on Nanion’s SURFE²R N1"

Icon N1   SURFE2R N1 application note   logo pdf   (0.4 MB)

EAAT3 - "Electrophysiological recordings of EAAT3 (SLC1A1) activity on Nanion’s SURFE²R N1"

Icon N1   SURFE²R application note  logo pdf   (0.4 MB)

GAT1 - "Electrophysiological recordings of hGAT1 (SLC6A1) activity on Nanion’s SURFE²R N1"

Icon N1   SURFE²R application note  logo pdf   (0.4 MB)

Neurons - "Electrophysiological recordings of LGIC and AA transporters in iCell® GlutaNeurons"

icon pl   Patchliner,  icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) and   Icon N1   SURFE2R N1 application note:   logo pdf   (0.5 MB)
Cells were kindly provided by FUJIFILM Cellular Dynamics, Inc.  

PepT1 - "Electrophysiological recordings of PepT1 (SLC15A1) activity on Nanion’s SURFE²R"

Icon N1   SURFE²R N1 application note:   logo pdf   (0.9 MB)

产品单页

SURFE2R N1 - 产品单页

Icon N1   SURFE²R N1 产品单页   logo pdf   (1.0 MB)

快速指南

发表文献

2020 - Unlocking the Reversal Potential of Solid Supported Membrane Electrophysiology to Determine Transport Stoichiometry

Icon N1   SURFE²R N1 pre-publication in bioRxiv (2020)

Authors:
Henzler-Wildman K.A., Thomas N.E.

2020 - Protein Adsorption on Solid Supported Membranes: Monitoring the Transport Activity of P-Type ATPases

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review in Molecules (2020)

Authors:
Tadini-Buoninsegni F.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level (2)

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors: 
Tadini-Buoninsegni F.,Palchetti I.

2020 - Energy Coupling in Cation-Pumping Pyrophosphatase—Back to Mitchell

Icon N1   SURFE²R N1 publication in Frontiers in Plant Science (2020)

Authors:
Baykov A.A.

2020 - Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis

icon sp96 SURFE2R N1 publication in Membranes (2020)

Authors:
Gantner M., Laftsoglou T., Rong H., Postis V.L.G., Jueken L.J.C.

2020 - A two-lane mechanism for selective biological ammonium transport

Icon N1   SURFE²R N1 publication in eLife (2020)

Authors:
Williamson G., Tamburrino G., Bizior A., Boeckstaens M., Mirandela G.D., Bage M.G., Pisliakov A., Ives C.M., Terras E., Hoskisson P.A., Marini A.M., Zachariae U., Javelle A.

2019 - Phosphatidylserine flipping by the P4-ATPase ATP8A2 is electrogenic

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PNAS (2019)

Authors:
Tadini-Buoninsegni F., Mikkelsen S.A., Mogensen L.S., Molday R.S., and Andersen J.P.

2019 - Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

Icon N1   SURFE²R N1 publication in Nature Scientific Reports (2019)

Authors:
Patiño-Ruiz M., Fendler K., & Călinescu O.

2019 - Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

Icon N1   SURFE2R N1 and   Icon Orbit   Orbit 16 publication in Frontiers in Pharmacology (2019)

Authors:
Dondapati S.K., Lübberding H., Zemella A., Thoring L., Wüstenhagen D.A., Kubick S.

2019 - A two-lane mechanism for selective biological ammonium transport

Icon N1   SURFE²R N1 pre-publication in bioRxiv (2019)

Authors:
Williamson G., Tamburrino G., Mirandela G.D., Boeckstaens M., Bage M., Pisliakov A., Ives C.M., Terras E.T., Bizior A., Hoskisson P.A., Marini A.M., Zachariae U., Javelle A.

2018 - The lipid environment determines the activity of the E. coli ammonium transporter, AmtB

Icon N1   SURFE²R N1 publication in Faseb J. (2018)

Authors:
Mirandela G.D., Tamburrino G., Hoskisson P.A., Zachariae U., Javelle A.

2017 - SSM-Based Electrophysiology for Transporter Research

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE book chapter in Methody in Enzymology

Authors:
Bazzone A., Barthmes M., Fendler K.

2017 - Mechanisms of charge transfer in human copper ATPases ATP7A and ATP7B

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in IUBMB Life (2017)

Authors:
Tadini-Buoninsegni F., Smeazzetto S.

2017 - Insights into the mechanism of membrane pyrophosphatases by combining experiment and computer simulation

Icon N1  SURFE²R N1 publication in Structural Dynamics (2017)

Authors: 
Shah N.R., Wilkinson C., Harborne S.P.D., Turku A., Li K.-M., Sun Y.-J., Harris S., Goldman A.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Discovery of Compounds that Positively Modulate the High Affinity Choline Transporter

Icon N1  SURFE²R N1 publication in Frontiers in Molecular Neuroscience (2017)

Authors: 
Choudhary P., Armstrong E.J., Jorgensen C.C., Piotrowski M., Barthmes M., Torella R., Johnston S.E., Maruyama Y., Janiszewski J.S., Storer R.I., Skerratt S.E., Benn C.L.

2017 - Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2017)

Authors:
Smeazzetto S., Armanious G.P., Moncelli M.R., Bak J.J., Lemieux M.J., Young H.S., Tadini-Buoninsegni F.

2017 - Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

Icon N1  SURFE²R-technology (custom-built system)  publication in PLoS ONE (2017)

Authors:
Patiño-Ruiz M., Ganea C., Fendler K., Călinescu O.

2016 - pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases

Icon N1  SURFE²R-technology (custom-built system) publication in PLoS ONE (2016)

Authors:
Bazzone A., Madej M.G., Kaback H.R., Fendler K.

2016 - Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism

Icon N1  SURFE²R N1 publication in Nature Communications (2016)

Authors: 
Li K., Wilkinson C., Kellosalo J., Tsai J., Kajander T, Jeuken L.J.C., Sun Y., Goldman A.

2016 - Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2016)

Authors:
Srinivasan L., Baars T.L., Fendler K., Michel H.

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2016 - Electrogenic Cation Binding in the Electroneutral Na+/H+ Antiporter of Pyrococcus abyssi

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2016)

Authors:
Călinescu O., Linder M., Wöhlert D., Yildiz Ö., Kühlbrandt W., Fendler K.

2016 - Charge translocation by mitochondrial NADH:ubiquinone oxidoreductase (complex I) from Yarrowia lipolytica measured on solid-supported membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical and Biophysical Research Communications (2016)

Authors:
Siebels I., Dröse S.

2015 - Structural and Functional Studies of NirC from Salmonella typhimurium

Icon N1   SURFE²R-technology (custom-built system) publication in Methods in Enzymology (2015)

Authors:
Rycovska-Blume A., Lü W., Andrade S., Fendler K., Einsle O.

2015 - Hofmeister effect of anions on calcium translocation by sarcoplasmic reticulum Ca2+-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Nature Scientific Reports (2015)

Authors:
Tadini-Buoninsegni F., Moncelli M.R., Peruzzi N., Ninham B.W., Dei L., Lo Nostroa P.

2015 - A universal mechanism for transport and regulation of CPA sodium proton exchangers

Icon N1  SURFE²R-technology (custom-built system) publication in Biological Chemistry (2015)

Authors:
Călinescu O., Fendler K.

2015 - A sulfur‐based transport pathway in Cu+‐ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in EMBO Reports (2015)

Authors:
Mattle D., Zhang L., Sitsel O., Pedersen L.T., Moncelli M.R., Tadini-Buoninsegni F., Gourdon P., Rees D.C., Nissen P., Meloni G.

2014 - Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling

Icon N1  SURFE²R-technology (custom-built system) publication in Nature Communications (2014)

Authors:
Perez C., Faust B., Mehdipour A.R., Francesconi K.A., Forrest L.R., Ziegler C.

2014 - Species differences in bacterial NhaA Na+/H+ exchangers

Icon N1  SURFE²R-technology (custom-built system) publication in FEBS Letters (2014)

Authors:
Călinescu O., Danner E., Böhm M., Hunte C., Fendler K..

2014 - NhaA Na+/H+ antiporter mutants that hardly react to the membrane potential

Icon N1  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Alkoby D., Rimon A., Budak M., Patino-Ruiz M., Călinescu O., Fendler K., Padan E..

2014 - Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium

Icon N1  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Lentes C.J., Mir S.H., Boehm M., Ganea C., Fendler K., Hunte C.

2014 - Keeping it simple, transport mechanism and pH regulation in Na+/H+ exchangers

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2014)

Authors:
Călinescu O., Paulino C., Kühlbrandt W., Fendler K.

2014 - Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch 96 Using Instant Frozen Cells

  Icon 96SE  SURFE²R N96 (predecessor model of SURFE²R 96SE) book chapter in Patch-Clamp Methods and Protocols

Authors:
Polonchuk L.

2014 - Direct observation of electrogenic NH4(+) transport in ammonium transport (Amt) proteins

Icon N1   SURFE²R-technology (custom-built system) publication in PNAS (2014)

Authors:
Wacker T., Garcia-Celma J.J., Lewe P., Andrade S.L.

2014 - Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP-Dependent Ca2+ Translocation by Sarco-Endoplasmic Reticulum Ca2+-ATPase (SERCA)

Icon N1  SURFE²R N1 publication in ChemMedChem (2014)

Authors: 
Sadafi F.Z., Massai L., Bartolommei G., Moncelli M.R., Messori L., Tadini-Buoninsegni F.

2013 - Photocycle and vectorial proton transfer in a rhodopsin from the eukaryote Oxyrrhis marina

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2013)

Authors:
Janke C., Scholz F., Becker-Baldus J., Glaubitz C., Wood P.G., Bamberg E., Wachtveitl J., Bamann C.

2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)

Authors:
Garcia-Celma J., Szydelko A., Dutzler R.

2013 - Enhanced adsorption of Ca-ATPase containing vesicles on a negatively charged solid supported membrane for the investigation of membrane transporters

Icon N1   SURFE²R N1 publication in Langmuir (2013)

Authors: 
Sacconi A., Moncelli M.R., Mergheri G., Tadini-Buoninsegni F.

2013 - Electrophysiological characterization of uncoupled mutants of LacY

Icon N1  SURFE²R-technology (custom-built system) publication in Biochemistry (2013)

Authors:
Gaiko O., Bazzone A., Fendler K., Kaback H.R.

2013 - Differential Effects of Mutations on the Transport Properties of the Na+/H+ Antiporter NhaA from Escherichia coli*

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2013)

Authors:
Mager T., Braner M., Kubsch B., Hatahet L., Alkoby D., Rimon A., Padan E., Fendler K.

2012 - The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2012)

Authors:
Rycovska A., Hatahet L., Fendler K., Michel H.

2012 - Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP

Icon N1  SURFE²R-technology (custom-built system) publication in PNAS (2012)

Authors:
Khafizov K., Perez C., Koshy C., Quick M., Fendler K., Ziegler C., Forrest L.R.

2012 - Distinctive features of catalytic and transport mechanisms in mammalian sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) and Cu+ (ATP7A/B) ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2012)

Authors:
Lewis D., Pilankatta R., Inesi G., Bartolommei G., Moncelli M.R., Tadini-Buoninsegni F.

2012 - Assaying the proton transport and regulation of UCP1 using solid supported membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in European Biophysics Journal (2012)

Authors:
Blesneac I., Ravaud S., Machillot P., Zoonens M., Masscheylen S., Miroux B., Vivaudou M., Pebay-Peyroula E.

2011 - Transport Mechanism and pH Regulation of the Na+/H+ Antiporter NhaA from Escherichia coli

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2011)

Authors:
Mager T., Rimon A., Padan E., Fendler K.

2011 - Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

Icon N1  SURFE²R ONE (a predecessor model of the SURFE²R N1) publication in Australian Journal of Chemistry (2011)

Authors:
Gaiko O., Janausch I., Geibel S., Vollert H., Arndt P., Gonski S., Fendler K.

2011 - G117C MelB, a mutant melibiose permease with a changed conformational equilibrium

Icon N1  SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Ganea C., Meyer-Lipp K., Lemonnier R., Krah A., Leblanc G., Fendler K.

2011 - Development of an assay for Complex I/Complex III of the respiratory chain using solid supported membranes and its application in mitochondrial toxicity screening in drug discovery

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2011)

Authors:
Preissl S., Bick I., Obrdlik P., Diekert K., Gul S., Gribbon P.

2011 - Characterisation of the purified human sodium/iodide symporter reveals that the protein is mainly present in a dimeric form and permits the detailed study of a native C-terminal fragment

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Huc-Brandt S., Marcellin D., Graslin F., Averseng O., Bellanger L., Hivin P., Quemeneur E., Basquin C., Navarro V., Pourcher T., Darrouzet E.

2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)

Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.

2010 - Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Pflügers Archiv - European Journal of Physiology (2010)

Authors:
Balannik V., Obrdlik P., Inayat S., Steensen C., Wang J., Rausch J.M., DeGrado W.F., Kelety B., Pinto L.H.

2010 - Electrophysiology of respiratory chain complexes and the ADP-ATP exchanger in native mitochondrial membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2010)

Authors:
Watzke N., Diekert K., Obrdlik P.

2010 - Electrophysiological characterization of ATPases in native synaptic vesicles and synaptic plasma membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical Journal (2010)

Authors:
Obrdlik P., Diekert K., Watzke N., Keipert C., Pehl U., Brosch C., Boehm N., Bick I., Ruitenberg M., Volknandt W., Kelety B.

2010 - Delineating electrogenic reactions during lactose/H+ symport

Icon N1  SURFE²R-technology (custom-built system) publication in Biochemistry (2010)

Authors:
Garcia-Celma J.J., Ploch J., Smirnova I., Kaback H.R., Fendler K.

2010 - ATP dependent charge movement in ATP7B Cu+-ATPase is demonstrated by pre-steady state electrical measurements

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2010)

Authors:
Tadini-Buoninsegni F., Bartolommei G., Moncelli M.R., Pilankatta R., Lewis D., Inesi G.

2009 - Measuring Ion Channels on Solid Supported Membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2009)

Authors:
Schulz P., Dueck B., Mourot A., Hatahet L., Fendler K.

2009 - Inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Chemical Research in Toxicology (2009)

Authors:
Gramigni E., Tadini-Buoninsegni F., Bartolommei G., Santini G., Chelazzi G., Moncelli M.R.

2009 - Electrophysiological characterization of LacY

Icon N1  SURFE²R-technology (custom-built system) publication in PNAS (2009)

Authors:
Garcia-Celma J.J., Smirnova I.N., Kaback H.R., Fendler K.

2009 - Electrogenic ion pumps investigated on a solid supported membrane: comparison of current and voltage measurements

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Langmuir (2009)

Authors:
Bartolommei G., Moncelli M.R., Rispoli G., Kelety B., Tadini-Buoninsegni F.

2009 - DtpB (YhiP) and DtpA (TppB, YdgR) are prototypical proton-dependent peptide transporters of Escherichia coli

Icon N1  SURFE²R ONE (a predecessor model of SURFE²R N1) publication in The FEBS Journal (2009)

Authors:
Harder D., Stolz J., Casagrande F., Obrdlik P., Weitz D., Fotiadis D., Daniel H.

2009 - An automatic electrophysiological assay for the neuronal glutamate transporter mEAAC1

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of Neuroscience Methods (2009)

Authors:
Krause R., Watzke N., Kelety B., Dörner W., Fendler K.

2008 - Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane

Icon N1  SURFE²R-technology (custom-built system) publication in Langmuir (2008)

Authors:
Garcia-Celma J.J., Dueck B., Stein M., Schlueter M., Meyer-Lipp K., Leblanc G., Fendler K.

2008 - Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2008)

Authors:
Wimmer F., Oberwinkler T., Bisle B., Tittor J., Oesterhelt D.

2008 - Effect of Clotrimazole on the Pump Cycle of the Na,K-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2008)

Authors:
Bartolommei G., Devaux N., Tadini-Buoninsegni F., Moncelli M., Apell H.-J.

2007 - Functional and structural characterization of a prokaryotic peptide transporter with features similar to mammalian PEPT1

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2007)

Authors:
Weitz D., Harder D., Casagrande F., Fotiadis D., Obrdlik P., Kelety B., Daniel H.

2006 - Transporter assays using solid supported membranes: a novel screening platform for drug discovery

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2006)

Authors:
Kelety B., Diekert K., Tobien J., Watzke N., Dörner W., Obrdlik P., Fendler K.

2006 - The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2006)

Authors:
Meyer-Lipp K., Séry N., Ganea C., Basquin C., Fendler K., Leblanc G.

2006 - Structure and function of prokaryotic glutamate transporters from Escherichia coli and Pyrococcus horikoshii

Icon N1   SURFE²R-technology (custom-built system) publication in Biochemistry (2006)

Authors:
Raunser S., Appel M., Ganea C., Geldmacher-Kaufer U., Fendler K., Kühlbrandt W.

2006 - Establishment of Cell-Free Electrophysiology for Ion Transporters: Application for Pharmacological Profiling

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biomolecular Screening (2006)

Authors:
Geibel S., Flores-Herr N., Licher T., Vollert H.

2005 - Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli

Icon N1   SURFE²R 500 (a predecessor model of SURFE²R N1) publication in Biochimica et Biophysica Acta (BBA) - Bioenergetics (2005)

Authors:
Zuber D., Krause R., Venturi M., Padan E., Bamberg E., Fendler K.

2004 - Time-resolved charge translocation by sarcoplasmic reticulum Ca-ATPase measured on a solid supported membrane

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2004)

Authors:
Tadini Buoninsegni F., Bartolommei G., Moncelli M.R., Inesi G., Guidelli R.

2004 - Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of Molecular Biology (2004)

Authors:
Zhou A., Wozniak A., Meyer-Lipp K., Nietschke M., Jung H., Fendler K.

2004 - Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of Molecular Biology (2004)

Authors:
Zhou A., Wozniak A., Meyer-Lipp K., Nietschke M., Jung H., Fendler K.

2004 - Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane

Icon N1   SURFE²R-technology (custom-built system) publication in Bioelectrochemistry (2004)

Authors:
Bartolommei G., Buoninsegni F.T., Moncelli M.R.

2003 - Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2003)

Authors:
Burzik C., Kaim G., Dimroth P., Bamberg E., Fendler K.

2002 - Photocurrents Generated by Bacteriorhodopsin Adsorbed on Thiol/Lipid Bilayers Supported by Mercury

Icon N1   SURFE²R-technology (custom-built system) publication in Langmuir (2002)

Authors:
Dolfi A., Tadini-Buoninsegni F., Moncelli M.R., Guidelli R.

2001 - Evidence for intraprotein charge transfer during the transport activity of the melibiose permease from Escherichia coli

Icon N1  SURFE²R-technology (custom-built system) publication in Biochemistry (2001)

Authors:
Ganea C., Pourcher T., Leblanc G., Fendler K.

1999 - Kinetics of electrogenic transport by the ADP/ATP carrier

Icon N1  SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Gropp T., Brustovetsky N., Klingenberg M., Müller V., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na/K-ATPase Investigated on Solid Supported Membranes: Rapid Solution Exchange with a New Technique

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K.

1993 - Charge transport by ion translocating membrane proteins on solid supported membranes

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1993)

Authors:
Seifert K., Fendler K., Bamberg E.

海报

2020 - A novel approach to detect electrogenic transporter activity in intact cells applied to investigate iPSC derived cardiomyocytes and neurons

 Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE poster, 64th Annual Meeting of the Biophysical Society   logo pdf   (1.6 MB)

2020 - Drug effects on human Na+/Ca2+ exchanger and implications for testing new drug candidates

 Icon N1  SURFE²R N1 and   Icon 96SE   SURFE²R 96SE poster, Safety Pharmacology Society Virtual Meeting 2020   logo pdf   (1.2 MB)

2018 - Label-free analysis of Na+/Ca2+- exchanger (NCX) isolated from iPSC-derived cardiomyocytes

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE2R 96SE poster, Europhysiology Meeting 2018  logo pdf   (1.5 MB)

2018 - Transported by light: optogenetic control of NCX1

Icon N1   SURFE²R N1 poster, Biophysics Annual Meeting 2018  logo pdf   (2.2 MB)

2015 - Organellar Transporters and Ion Channels - How to access their electrophysiology by using the SURFE2R technology and Planar Patch Clamp

Icon N1   SURFE²R N1 and   icon sp96   SyncroPatch 96 (a predecessor model of the SyncroPatch 384PE) and   icon pap   Port-a-Patch poster, GRC - Organellar Channels and Transporters 2015   logo pdf   (1.6 MB)

2017 - An emerging technique for the characterization of transport proteins: SSM-based electrophysiology

Icon N1   SURFE²R N1 and    Icon 96SE   SURFE²R SE96 poster, 19th IUPAB / 11th EBSA congress 2017  logo pdf   (3.3 MB)

2015 - Functional Characterization of Prokaryotic NCX by Solid Supported Membrane Technology

Icon N1   SURFE²R N1 poster, Gordon Research Conference 2015  logo pdf   (0.9 MB)

演讲文档

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