2016 - Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2016)

Srinivasan L., Baars T.L., Fendler K., Michel H.

Biochimica et Biophysica Acta (BBA) - Biomembranes (2016) 1858(4):698-705


  • SLC 26 family transporters may be multifunctional and may transport different anions depending on the conditions.
  • Electrogenic, chloride dependent transport events were recorded in liposomes.
  • Fumarate is a low-affinity substrate for StmYchM/DauA.
  • Bicarbonate is transported with a high flux at alkaline pH.


Solute carrier (SLC) 26 or sulfate permease (SulP) anion transporters, belong to a phylogenetically ancient family of secondary active transporters. Members of the family are involved in several human genetic diseases and cell physiological processes. Despite their importance, the substrates for transport by this family of proteins have been poorly characterized. In this study, recombinant StmYchM/DauA, a SulP from Salmonella typhimurium was purified to homogeneity and functionally characterized. StmYchM/DauA was found to be a dimer in solution as determined by size exclusion chromatography coupled to multiple angle light scattering. We report a functional characterization of the SulP proteins in two membrane mimetic systems and reveal a dual nature of anionic substrates for SulP. StmYchM/DauA functionally incorporated into nanodiscs could bind fumarate with millimolar affinities (KD = 4.6 ± 0.29 mM) as detected by intrinsic tryptophan fluorescence quench studies. In contrast, electrophysiological experiments performed in reconstituted liposomes indicate a strong bicarbonate transport in the presence of chloride but no detectable electrogenic fumarate transport. We hence suggest that while SulP acts as an electrogenic bicarbonate transporter, fumarate may serve as substrate under different conditions indicating multiple functions of SulP.

Download here


We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.