Vesicle Prep Pro -囊泡制备仪

Dr. Eric Stava - Statement about the Vesicle Prep Pro

   “The Vesicle Prep Pro has been of great utility in our Bioelectronics research, allowing students and researchers with limited electrophysiology background to easily form giant unilamellar vesicles (GUVs).  The electronic control lets us alter the size and density of the GUVs, and the transparent chamber allows for excellent visualization of the electro-swelling process.”

Eric Stava, PhD, Research Assistant
University of Wisconsin-Madison, WI, US

Dr. Marisa Rangel - Statement about the Vesicle Prep Pro and Port-a-Patch

      “Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro setup. Together with the Port-a-Patch, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings.”

Marisa Rangel, PhD, Researcher 
University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil, WI, US

Prof. Dr. Colin Taylor - Statement about the Port-a-Patch and Vesicle Prep Pro

      “We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.”

Colin Taylor, Professor of  Cellular Pharmacology, Department of Pharmacology
University of Cambridge, Cambridge, UK

GUVs - Average Diameter

   Vesicle Prep Pro data and applications:
The Image was kindly provided of Dr. Sonderman/Prof. Behrends, University of Freiburg, Germany.

The GUVs produced with the Vesicle Prep Pro typically range from 1 – 30 μm in diameter, with an average diameter of
approximately 10 μm (60-70%).

Gramicidin - Rapid Internal Solution Exchange

   Port-a-Patch and     Vesicle Prep Pro data and applications:

Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.

MscL - Pressure Clamp Experiments

  Port-a-Patch and     Vesicle Prep Pro data and applications:

The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.

Bilayer Recordings - Low Noise

   Port-a-Patch and     Vesicle Prep Pro data and applications:

Typical recording of the Irms noise level of a bilayer (DPhPC) on the Low Capacitance Holder. Typical values are 220 fA

(10 kHz filter frequency, 50 kHz sampling rate. Cf. 2.3 pA RMS on the standard Port-a-Patch).

Alpha-Hemolysin - DNA Translocation

   Port-a-Patch and      Vesicle Prep Pro data and applications:

Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.

Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch.

TRPM8 - Bilayer Recording

  Port-a-Patch and     Vesicle Prep Pro data and applications: 
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA

Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.

KcsA - Bilayer Recordings

   Vesicle Prep Pro data and applicatons:

The image shows a representative current trace recorded from a planar lipid bilayer in which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7). A ramp from -100 mV to +100 mV was used to evoke the single channel events.

Download: Application Note

MscL - Pressure Activation

  Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Boris Martinac, University of QLD, Brisbane, Australia.

Pressure activation of MscL. The MscL channel is gated by pressure, where single channel activity was recorded at 30 mV at the indicated negative hydrostatic pressure. Nanion provides a dedicated suction control device for pressure clamp, found under the Port-a-Patch section.

OmpF - Bilayer Recordings

   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany.

Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine. 

Inositol-Triphosphate 3 Receptor (IP3) - Reconstitution

  Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK.

Representative traces of the IP₃ (inositol-triphosphate) receptor were recorded at different voltages in the presence of IP₃ (0.2 mM free Ca²⁺ and 1 mM Na₂ATP, 140 mM KCl). Addition of 2 μM IP₃ on the cytosolic side evoked openings of the IP₃ receptor.

Cytolysin (Bacterial) - Bilayer Recordings

   Port-a-Patch and     Vesicle Prep Pro data and applications:
Recordings were kindly supplied by ITC & CNR-Istituto di Biofisica, Trento, Italy.

Traces were recorded in 100 mM KCl, 10 mM Hepes, 0.1 mM EDTA, pH 7 at -40 mV. Lipid: Diphtanoyl-PC.

Alamethicin - Bilayer Recordings

   Port-a-Patch and     Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.

Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.

Gramicidin - Single Channel Analysis

   Port-a-Patch and      Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.

Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.

Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings

  Port-a-Patch and     Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.

Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.

KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

   Port-a-Patch and      Vesicle Prep Pro application note:     (0.2 MB)

preview the file here for download

MscL - "Lipid bilayer recordings of a mechanosensitive channel, MscL, using Nanion’s pressure clampchannel, MscL, using Nanion’s pressure clamp"

  Port-a-Patch and      Vesicle Prep Pro application note      (0.3 MB)

preview the file here for download

OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "

   Port-a-Patch and      Vesicle Prep Pro application note:      (1.4 MB) 

preview the file here for download

Vesicle Prep Pro - 产品单页

  Vesicle Prep Pro 产品单页:      (2.6 MB)

预览文件下载

2020 - The structure of the antimicrobial human cathelicidin LL-37 shows oligomerization and channel formation in the presence of membrane mimics

 Port-a-Patch and   Vesicle Prep Pro publication in Scientific Reports (2020)

Authors:
Sancho-Vaello E., Gil-Carton D., François P., Bonetti E.J., Kreir M., Pothula K.R., Kleinekathöfer U., Zeth K.

2020 - The mechanisms of action of water-soluble aminohexanoic and malonic adducts of fullerene C60 with hexamethonium on model lipid membranes

   Vesicle Prep Pro Publication found in Biochimica et Biophysica Acta (BBA) -  Biomembranes (2020)

Authors:
Efimova S.S., Khaleneva D.A., Litasova E.V., Piotrovskiy L.B., Ostroumova O.S.

2020 - The Disordering Effect of Plant Metabolites on Model Lipid Membranes of Various Thickness

 Vesicle Prep Pro publication in Cell and Tissue Biology (2020)

Authors:
Efimova S.S., Ostroumova O.S.

2020 - Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity

   Orbit mini and      Vesicle Prep Pro publication in Science Advances (2020)

Authors:
Ni T., Jiao F., Yu X., Aden S., Ginger L., Williams S.I., Bai F., Pražák V., Karia D., Stansfeld P., Zhang P., Munson G., Anderluh G.,Scheuring S., Gilbert R.J.C.

2020 - Romo1-Derived Antimicrobial Peptide Is a New Antimicrobial Agent against Multidrug-Resistant Bacteria in a Murine Model of Sepsis

   Vesicle Prep Pro publication in mBio (2020)

Authors:
Lee H.-R., You D.-G., Kim H.K., Sohn J.W., Kim M.J., Park J.K., Lee G.Y., Yoo Y.D.

2020 - Purification and initial characterization of Plasmodium falciparum K+ channels, PfKch1 and PfKch2 produced in Saccharomyces cerevisiae

 Vesicle Prep Pro and  Port-a-Patch publication in Microbial Cell Factories (2020)

Authors:
Molbaek K., Tejada M., Ricke C.H., Scharff-Poulsen P., Ellekvist P., Helix-Nielsen C., Kumar N., Klaerke D.A., Pedersen P.A.

2020 - Possible Mechanisms of Toxicity of Local Aminoamide Anesthetics: Lipid-Mediated Action of Ropivacaine

   Vesicle Prep Pro publication in Cell and Tissue Biology (2020)

Authors:
Zakharova A. A., Efimova S.S., Koryachkin V.A., Zabolotskii D.V., Ostroumova O.S. 

2020 - Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells

   Vesicle Prep Pro publication in Small (2020)

Authors:
Brodszkij E., Westensee I.N., Bertelsen M., Gal N., Boesen T.,Städler B.

2020 - Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes

   Vesicle Prep Pro publication in eLife (2020)

Authors:
Ohashi Y., Tremel S., Masson G.R., McGinney L., Boulanger J., Rostislavleva K., Johnson C.M., Niewczas I., Clark J., Williams R.J.

2020 - Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes

  Port-a-Patch and      Vesicle Prep Pro publication in Nature Communications (2020)

Authors: 
Debnath M., Chakraborty S., Kumar Y.P., Chaudhuri R., Jana B., Dash J.

2020 - Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus

 Vesicle Prep Pro publication in Viruses (2020)

Authors:
Alsaadi E.A.J., Neuman B.W., Jones I.M.

2020 - Hybrid giant lipid vesicles incorporating a PMMA-based copolymer

   Vesicle Prep Pro Publication found in Biochimica et Biophysica Acta (BBA) - General Subjects (2020)

Authors:
Miele Y., Mingotaud A.F., Caruso E., Malacarne M. C., Izzo L., Lonetti B., Rossi F.

2020 - Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel

  Port-a-Patch and      Vesicle Prep Pro publication in Cell Calcium (2020)

Authors: 
Moparthi L., Zygmunt P.M.

2020 - Hepatitis C Virus p7 Induces Membrane Permeabilization by Interacting with Phosphatidylserine

  Port-a-Patch and      Vesicle Prep Pro publication in International Journal of Molecular Sciences (2020)

Authors: 
Lee H-R., Lee G.Y., You D-G., Kim H.K., Yoo Y.D.

2020 - Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures

   Vesicle Prep Pro publication in Polymers (2020)

Authors:
Khan A., Ho J.C.S., Roy S., Bo Liedberg B., Nallani M.

2020 - Exploring the Relationship between BODIPY Structure and Spectroscopic Properties to Design Fluorophores for Bioimaging

   Vesicle Prep Pro publication in Chemistry a European Journal (2020)

Authors:
Donnelly J.L., Offenbartl-Stiegert D., Marín-Beloqui J.M., Rizzello L., Battaglia G., Clarke T.M., Howorka S., Wilden J.D.

2020 - Electrophysiological characterization of transport across outer membrane channels from Gram‐negative bacteria in presence of lipopolysaccharides (LPS)

   Port-a-Patch,     Vesicle Prep Pro and      Orbit mini Publication in Angewandte Chemie International Edition (2020)

Authors:
Wang J., Terrasse R., Bafna J.A., Benier L., Winterhalter M.

2020 - Effect of DMSO on the Mechanical and Structural Properties of Model and Biological Membranes

    Vesicle Prep Pro publication in the Biophysical Journal (2020)

Authors:
Gironi B., Kahveci Z., McGill B., Lechner B.D., Pagliara S., Metz J., Morresi A., Palombo F., Sassi P., Petrov P.G.

2020 - Destructing the Plasma Membrane with Activatable Vesicular DNA Nanopores

    Vesicle Prep Pro Publication in ACS Appl. Mater. Interfaces (2020)

Authors:
Chen L, Liang S., Chen Y., Wu M., Zhang Y.

2020 - Design of Protein Logic Gate System Operating on Lipid Membranes

    Vesicle Prep Pro Publication in ACS Synth. Biol. (2020)

Authors:
Omersa N., Aden S., Kisovec M., Podobnik M., Anderluh G.

2020 - Design and Assembly of Membrane-Spanning DNA Nanopores

  Vesicle Prep Pro publication in Nanopore Technology (2020)

Authors:
Göpfrich K., Ohmann A., Keyser U.F.

2020 - Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR

 Port-a-Patch and   Vesicle Prep Pro publication in Nature Communications (2020)

Authors:
Iwahasi Y., Toyama Y., Imai S., Itoh H., Osawa M., Inoue M., Shimada I.

2020 - Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly

   Vesicle Prep Pro Publication found in ACS Nano (2020)

Authors:
Baumann, K.N., Piantanida L., García-Nafría J., Sobota D., Voïtchovsky K., Knowles T.P.J., Hernández-Ainsa S.

2020 - Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality

   Vesicle Prep Pro publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2020)

Authors:
de las Mercedes Carro M., Peñalva D.A., Antollini S.S., Hozbora F.A., Buschiazzo J.

2020 - Characterization of lipid composition and diffusivity in OLA generated vesicles

    Vesicle Prep Pro publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2020)

Authors:
Schaich M., Sobota D., Sleath H., Cama J., Keyser U.F.

2020 - Calcium activates purified human TRPA1 with and without its N-terminal ankyrin repeat domain in the absence of calmodulin

   Port-a-Patch and      Vesicle Prep Pro publication in Cell Calcium (2020)

Authors: 
Moparthi L., Moparthi S.B., Wenger J., Zygmunt P.M.

2020 - Atg3 promotes Atg8 lipidation via altering lipid diffusion and rearrangement

   Vesicle Prep Pro publication in Protein Science (2020)

Authors:
Wang S., Li Y., Ma C.

2020 - Actuation of synthetic cells with proton gradients generated by light-harvesting E. coli

  Vesicle Prep Pro prepublication in Research Square (2020)

Authors:
Jahnke K., Ritzmann N., Fichtler J., Nitschke A., Dreher Y., Abele T., Hofhaus G., Platzman I., Schröder R., Muller D., Spatz J., Göpfrich K.

2019 - The Mechanisms of Action of Triindolylmethane Derivatives on Lipid Membranes

    Vesicle Prep Pro Publication in Acta Naturae (2019)

Authors:
Efimova, S.S. Tertychnaya T.E., Lavrenov S.N., Ostroumova, O.S.

2019 - Temporin L and aurein 2.5 have identical conformations but subtly distinct membrane and antibacterial activities

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports (2019)

Authors:
Manzo G., Ferguson P.M., Hind C.H., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton J.M., Lorenz C.D., Phoenix D.A., Mason A.J.

2019 - Structure of the human ClC-1 chloride channel

   Port-a-Patch,     Vesicle Prep Pro and      Orbit mini publication in PLOS Biology (2019)

Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.

2019 - Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module

    Vesicle Prep Pro Pre-publication in BioRxiv (2019)

Authors:
Maksimov E.G., Zamaraev A.V., Parshina E.Y., Slonimskiy Y.B., Slastnikova T.A., Abdrakhmanov A.A., Babaev P.A., Efimova, S.S., Ostroumova O.S., Stepanov A.V., Ryabova, A.V., Friedrich T., Sluchanko N.N.

2019 - Self‐Assembly of Giant Unilamellar Vesicles by Film Hydration Methodologies

   Vesicle Prep Pro Publication in Advanced Biosystems (2019)

Authors:
Rideau E., Wurm F.R., Landfester K.

2019 - Protein-free division of giant unilamellar vesicles controlled by enzymatic activity

    Vesicle Prep Pro Pre-publication in BioRxiv (2019)

Authors:
Dreher Y., Spatz J.P., Göpfrich K.

2019 - Molecular understanding of calcium permeation through the open Orai channel

   Port-a Patch and      Vesicle Prep Pro Publication in PLOS Computational Biology (2019)

Authors:
Liu X., Wu G., Yu Y., Chen X., Ji R., Lu J., Li X., Zhang X., Yang X., Shen Y.

2019 - Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports (2019)

Authors:
Manzo G., Ferguson P.M., Gustilo V.B., Ali H., Bui T.T., Drake A.F., Atkinson R.A., Batoni G., Lorenz C.D., Phoenix D.A., Mason A.J.

2019 - Mechanisms of Regulation of Amyloid-Induced Permeability of Model Lipid Membranes by Polyphenols

    Vesicle Prep Pro Publication in Cell Tiss. Biol. (2019)

Authors:
Efimova, S.S. & Ostroumova, O.S.

2019 - Linker length in fluorophore–cholesterol conjugates directs phase selectivity and cellular localisation in GUVs and live cells

    Vesicle Prep Pro publication in RSC Advances (2019)

Authors:
O' Connor D., Byrnea A., Keyes T.E.

2019 - Hepatitis B virus X protein induces size-selective membrane permeabilization through interaction with cardiolipin

   Vesicle Prep Pro publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2019)

Authors:
You D.G., Cho Y.Y., Lee H.R., Lee J.H., Yu S.J., Yoon J.H., Yoo Y.D., Kim Y.J., Lee G.Y.

2019 - Golgi anti-apoptotic proteins are evolutionarily conserved ion channels that regulate cell death in plants

   Port-a-Patch and      Vesicle Prep Pro Pre-publication in BioRxiv (2019)

Authors:
Sierla M., Prole D.L., Saraiva N., Carrara G., Dinischiotu N., Vaattovaara A., Wrzaczek M., Taylor C.W., Smith F.L., Feys B.

2019 - Fluorescent Artificial Receptor-Based Membrane Assay (FARMA) for Spatiotemporally Resolved Monitoring of Biomembrane Permeability

   Vesicle Prep Pro Pre-Publication in ChemRxiv (2019)

Authors:
Biedermann F., Ghale F., Hennig A., Nau W.M.

2019 - Cellular uptake of self-assembled phytantriol-based hexosomes is independent of major endocytic machineries

    Vesicle Prep Pro publication in Journal of Colloid Interface Science (2019)

Authors:
Rodrigues L., Schneider F. , Zhang X., Larsson E., Moodie L., Dietz H., Papadakis C., Winter G., Lundmark R., Hubert M.

2019 - Barcoding biological reactions with DNA-functionalized vesicles

   Vesicle Prep Pro Pre-Publication in BioRxiv (2019)

Authors:
Peruzzi J.A., Jacobs, ML., Vu T.Q., Kamat N.P.

2019 - An Intrinsically Disordered Region in OSBP Acts as an Entropic Barrier to Control Protein Dynamics and Orientation at Membrane Contact Sites

   Vesicle Prep Pro publication in Developmental Cell (2019)

Authors:
Jamecna D., Polidori J., Mesmin B., Dezi M., Levy D., Bigay J., Antonny B.

2019 - A trifunctional linker for palmitoylation and peptide and protein localization in biological membranes

    Vesicle Prep Pro publication in ChemBioChem (2019)

Authors:
Syga L., De Vries R.H., van Oosterhout H., Bartelds R., Boersma A.J., Roelfes G., Poolman B.

2019 - A Fusion Peptide in the Spike Protein of MERS Coronavirus

   Vesicle Prep Pro Publication in Viruses (2019)

Authors:
Alsaadi E.A.J., Neuman B.W., and Jones I.M.

2018 - Visualizing Tension and Growth in Model Membranes Using Optical Dyes

   Vesicle Prep Pro publication in Biophysical Journal (2018)

Authors:
Boyd M.A., Kamat N.P.

2018 - Structures of monomeric and oligomeric forms of the Toxoplasma gondii perforin-like protein 1

   Orbit mini and      Vesicle Prep Pro publication in Science Advances (2018)

Authors:
Ni T., Williams S.I., Rezelj S., Anderluh G., Harlos K., Stansfeld P.J., Gilbert R.J.C.

2018 - Single channel recording of a mitochondrial calcium uniporter

   Port-a-Patch and      Vesicle Prep Pro publication in BBRC (2018)

Authors: 
Wu G., Li S., Zong G., Liu, X., Fei S., Shen L., Guan X., Yanga X., Shen Y.

2018 - Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics

   Port-a-Patch and      Vesicle Prep Pro publication in Journal of Cell Biology (2018)

Authors:
Lee G.Y., You D.G., Lee H.R., Hwang S.W., Lee C.J., Do Yoo Y.

2018 - Regulation of the Pore-Forming Activity of Cecropin A by Local Anesthetics

   Vesicle Prep Pro publication in Cell and Tissue Biology (2018)

Authors:
Efimova S.S., Medvedev R.Ya., Chulkov E.G., Schagina L.V., Ostroumova O.S.

2018 - Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers

   Port-a-Patch and      Vesicle Prep Pro publication in Current Protocols in Pharmacology (2018)

Authors:
Klaerke D.A., de los Angeles Tejada M., Grøsfjeld Christensen V., Lassen M., Amstrup Pedersen P., Calloe K.

2018 - Quantifying Permeation of Small Charged Molecules across Channels: Electrophysiology in Small Volumes

   Port-a-Patch and      Vesicle Prep Pro publication in ACS Omega (2018)

Authors:
Wang J., Benier L., Winterhalter M.

2018 - Probing Interactions between AuNPs/AgNPs and Giant Unilamellar Vesicles (GUVs) Using Hyperspectral Dark-field Microscopy

   Vesicle Prep Pro publication in the International Journal of Molecular Sciences (2018)

Authors:
Bhat A., Huan K., Cooks T., Boukari H., Lu Q.

2018 - Phase partitioning, solvent-switchable BODIPY probes for high contrast cellular imaging and FCS

   Vesicle Prep Pro publication in New Journal of Chemistry (2018)

Authors:
O’Connor D. Byrne A., Dolana C., Keyes T.E.

2018 - Parallel evolution of frog antimicrobial peptides produces identical conformations but subtly distinct membrane and antibacterial activities

   Port-a-Patch and      Vesicle Prep Pro pre-publication in bioRxiv (2018)

Authors:
Manzo G., Ferguson P.M., Hind C., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton M.J., Lorenz C.D., Phoenix D.A., Mason A.J.

2018 - Molecular mechanism of the Orai channel activation

   Port-a-Patch and      Vesicle Prep Pro Pre-publicatin in bioRxiv (2018)

Authors:
Liu, X., Wu, G., Yu, Y., Chen, X., Ji, R., Lu, J., Li, X., Zhang, X., Yang, X., Shen, Y.

2018 - Methods for Single-Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor

   Vesicle Prep Pro book chapter in Molecular Motors (2018)

Authors:
Haque F, Zhang H, Wang S, Chang C-L, Savran C, Guo P.

2018 - Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking

   Vesicle Prep Pro publication in Scientific Reports (2018)

Authors:
Syga Ł., Spakman D., Punter C.M., Poolman B.

2018 - Lipid-mediated regulation of pore-forming activity of syringomycin E by thyroid hormones and xanthene dyes

   Vesicle Prep Pro publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2018)

Authors:
Efimova S.S., Zakharova A.A., Ismagilov A.A., Schagina L.V., Malev V.V., Bashkirov P.V., Ostroumova O.S.

2018 - Hybrid vesicles from lipids and block copolymers: Phase behavior from the micro- to the nano-scale

   Vesicle Prep Pro publication in Colloids and Surfaces B: Biointerfaces (2018)

Authors:
Magnani C., Montis C., Mangiapia G., Mingotaud A.-F., Mingotaud C., Roux C., Joseph P., Berti D., Lonetti B.

2018 - High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope

   Vesicle Prep Pro publication in the Journal of Biophotonics (2018)

Authors:
Li Y., Montague S.J., Brüstle A., He X., Gillespie C., Gaus K., Gardiner E.E., Lee W.M.

2018 - Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

   Vesicle Prep Pro publication in Scientific Reports (2018)

Authors:
Startek J.B., Talavera K., Voets T., Alpizar Y.A.

2018 - Development of an on-chip antibiotic permeability assay with single molecule detection capability

   Vesicle Prep Pro publication in IEEE Transactions on NanoBioscience (2018)

Authors:
Guzel F.D., Citak F.

2018 - Cholesterol Enriched Archaeosomes as a Molecular System for Studying Interactions of Cholesterol-Dependent Cytolysins with Membranes

   Vesicle Prep Pro publication in The Journal of Membrane Biology (2018)

Authors:
Rezelj S., Kozorog M, Švigelj T., Ulrih N.P., Žnidaršič N., Podobnik M., Anderluh G.

2018 - Cargo induces retromer-mediated membrane remodeling on membranes

   Vesicle Prep Pro publication in ascb Molecular Biology of the Cell (2018)

Authors:
Purushothaman L.K., Ungermann C.

2018 - An aromatic cluster in Lysinibacillus sphaericus BinB involved in toxicity and proper in-membrane folding

   Port-a-Patch and      Vesicle Prep Pro publication in Archives of Biochemistry and Biophysics (2018)

Authors:
Chooduang A., Surya W., Torres J., Boonserm P.

2018 - Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage

   Vesicle Prep Pro publication in eLife (2018)

Authors:
Manni M.M., Tiberti M.L., Pagnotta S., Barelli H., Gautier R., Antonny B.

2018 - A synthetic enzyme built from DNA flips 10e7 lipids per second in biological membranes

   Vesicle Prep Pro publication in the Nature Communications (2018)

Authors:
Ohmann A, Li C-Y, Maffeo C, Al Nahas K, Baumann K.N, Göpfrich K, Yoo J, Keyser U.F, & Aksimentiev A.

2018 - A direct role for hepatitis B virus X protein in inducing mitochondrial membrane permeabilization

   Vesicle Prep Pro publication in Journal of Viral Hepatitis (2018)

Authors:
Lee H‐R., Cho Y.Y., Lee G.Y., You D‐g., Yoo Y.D., Kim Y.J.

2017 - The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports

Authors:
Pham T., Perry J.L., Dosey T.L., Delcour L.H.,Hyser J.M.

2017 - Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

   Port-a-Patch and      Vesicle Prep Pro publication in PLoS ONE (2017)

Authors: 
Rangel M., Fernandes dos Santos Castro F., Mota-Lima L.D., Clissa P.B., Martins D.B., Perez dos Santos Cabrera M., Mortari M.R.

2017 - Organelle membrane derived patches: reshaping classical methods for new targets

   Port-a-Patch and      Vesicle Prep Pro publication in Nature Scientific Reports (2017)

Authors:
Shapovalov G., Ritaine A., Bidaux G., Slomianny, C., Borowiec A-S., Gordienko D., Bultynck G., Skryma R., Prevarskaya N.

2017 - Lipid phase separation in the presence of hydrocarbons in giant unilamellar vesicles

   Vesicle Prep Pro publication in AIMS Biophysics (2017)

Authors:
Bartelds R., Barnoud J., Boersma A.J., Marrink S.J., Poolman B.

2017 - Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel

   Vesicle Prep Pro publication in BioMed Central Biology (2017)

Authors: 
Ou X., Guo J., Wang L., Yang H., Liu X., Sun J., Liu Z.

2017 - Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)

   Port-a-Patch and      Vesicle Prep Pro publication in International Journal of Antimicrobial Agents (2017)

Authors: 
Silva J.C., Neto L.M., Neves R.C., Gonçalves J.C., Trentini M.M., Mucury-Filho R., Smidt K.S., Fensterseifer I.C., Silva O.N., Lima L.D., Clissa P.B., Vilela N., Guilhelmelli F., Silva L.P., Rangel M., Kipnis A., Silva-Pereira I., Franco O.L., Junqueira-Kipnis A.P., Bocca A.L., Mortari M.R.

2017 - Engineering a pH responsive pore forming protein

  Orbit mini and      Vesicle Prep Pro publication in Scientific Reports (2017)

Authors: 
Kisovec M., Rezelj S., Knap P., Cajnko M.M., Caserman S., Flašker A., Žnidaršič N., Repič M., Mavri J., Ruan Y., Scheuring S., Podobnik M., Anderluh G.

2017 - Dipole Modifiers Regulate Lipid Lateral Heterogeneity in Model Membranes

   Vesicle Prep Pro publication in Acta Naturae (2017)

Authors:
Efimova S.S., Ostroumova O.S.

2017 - Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel

  Port-a-Patch and      Vesicle Prep Pro publication in Structure (2017)

Authors: 
Arya P, Jarerattanachat V., Clausen M.V., Schewe M., McClenaghan C., Argent L., Conrad L.J., Dong Y.Y., Pike A.C.W., Carpenter E.P., Baukrowitz T., Sansom M.S.P., Tucker S.J.

2017 - Asymmetric mechanosensitivity in a eukaryotic ion channel

   Port-a-Patch and      Vesicle Prep Pro publication in PNAS (2017)

Authors:
Clausen M.V., Jarerattanachata V., Carpenterc E.P., Mark S. , Sansomb P., Tucker S.J.

2016 - The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor

  Port-a-Patch and      Vesicle Prep Pro publication in Nature (2016)

Authors: 
Survery S., Moparthi L., Kjellbom P., Högestätt E.D., Zygmunt P.M., Johanson U.

2016 - Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola

  Port-a-Patch and      Vesicle Prep Pro publication in Nature Communications (2016)

Authors:
Dhakshnamoorthy B., Rohaim A., Rui H., Blachowicz L., Roux B.

2016 - Pore architecture of TRIC channels and insights into their gating mechanism

  Port-a-Patch and      Vesicle Prep Pro publication in Nature (2016)

Authors: 
Yang H., Hu M., Guo J., Ou X., Cai T., Liu Z.

2016 - Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1

  Port-a-Patch and      Vesicle Prep Pro publication in The Journal of Neuroscience (2016)

Authors: 
Babes A., Sauer S.K., Moparthi L., Kichko T.I., Neacsu C., Namer B., Filipovic M., Zygmunt P.M., Reeh P.W., Fischer M.J.

2016 - Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity

  Port-a-Patch and      Vesicle Prep Pro publication in Scientific Reports (2016)

Authors: 
Moparthi L., Kichko T. I., Eberhardt M., Högestätt E. D., Kjellbom P., Johanson U., Reeh P., W., Leffler A., Filipovic M. R., Zygmunt P. M.

2016 - Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system

  Port-a-Patch and      Vesicle Prep Pro publication in Protein Expression and Purification (2016)

Authors: 
Soranzo T., Cortès S., Gilde F., Kreir M., Picart C., Lenormand J.-L.

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

  SURFE²R N1 and      Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2016 - Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biomedical Science and Engineering (2016)

Authors: 
Sieber M., Kaltenbach S., Hanke W. and Kohn F.P.M

2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of the American Chemical Society (2015)

Authors: 
Cama J., Bajaj H., Pagliara S., Maier T., Braun Y., Winterhalter M., Keyser U.F.

2015 - High-Resolution Size-Discrimination of Single Nonionic Synthetic Polymers with a Highly Charged Biological Nanopore

  Orbit 16 and      Vesicle Prep Pro publication in American Chemical Society Nano (2015)

Authors: 
Baaken G., Halimeh I., Bacri, Pelta J., Oukhaled A., Behrends J.C.

2015 - Golgi anti-apoptotic proteins are highly conserved Ion Channels That Affect Apoptosis and Cell Migration

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

Authors: 
Carrara G., Saraiva N., Parsons M., Byrne B., Prole D.L., Taylor C.W., Smith G.L.

2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

Authors: 
Billen B., Brams M., Debaveye S., Remeeva A., Alpizar Y.A., Waelkens E., Kreir M., Brüggemann A., Talavera K., Nilius B., Voets T., Ulens C.

2015 - Cell-free expression of a functional pore-only sodium channel

  Port-a-Patch and      Vesicle Prep Pro publication in Protein Expression and Purification (2015)

Authors:
Kovácsová G, Gustavsson E, Wang J, Kreir M, Peuker S, Westenhoff S.

2015 - Bilayer-Spanning DNA Nanopores with Voltage- Switching between Open and Closed State

  Orbit 16 and      Vesicle Prep Pro publication in American Chemical Society Nano (2015)

Authors: 
Seifert A., Göpfrich K., Burns J.R., Fertig N., Keyser U.F., Howorka S.

2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

  Orbit 16,      Port-a-Patch and      Vesicle Prep Pro publication in Analyst (2015)

Authors:
Weichbrodt C., Bajaj H., Baaken G., Wang J., Guinot S., Kreir M, Behrends J.C., Winterhalter M., Fertig N.

2014 - Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

   Port-a-Patch and      Vesicle Prep Pro publication in Biomedical Optics Express (2014)

Authors:
Kniggendorf A.-K., Meinhardt-Wollweber M., Yuan X., Roth B., Seifert A., Fertig N., Zeilinger C.

2014 - Studying mechanosensitive ion channels with an automated patch clamp

  Port-a-Patch and      Vesicle Prep Pro publication in European Biophysics Journal (2014)

Authors:
Barthmes M.,  Mac Donald F.J., Birkner J.P., Brüggemann A., Wahl-Schott C., Koçer A.

2014 - Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biological Chemistry (2014)

Authors: 
Li Y., Surya W., Claudine S., Torres J.

2014 - Nanoscale phase behavior on flat and curved membranes

   Vesicle Prep Pro publication in Nanotechnology (2014)

Authors:
Andersen T., Bahadori A., Ott D., Kyrsting A., Reihani S.N.S., Bendix P.M.

2014 - Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

  Port-a-Patch and      Vesicle Prep Pro publication in Biosensors and Bioelectronics (2014)

Authors: 
Dondapati S.K., Kreir M., Quast R.B., Wüstenhagen D.A., Brüggemann A., Fertig N., Kubick S.

2014 - Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain

  Port-a-Patch and      Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

Authors: 
Moparthi L., Survery S., Kreir M., Simonsen C., Kjellbom P., Högestätt E.D., Johanson U., Zygmunt P.M.

2014 - An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity

  Port-a-Patch and      Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

Authors: 
Danilchanka A., Sun J., Pavlenok M., Maueröder C., Speer A., Siroy A., Marrero J., Trujillo C., Mayhew D.L., Doornbos K.S., Muñoz 'L.E., HerrmannM., EhrtS., Berens C., Niederweisa M.

2013 - Lipid-Bilayer-Spanning DNA Nanopores with a Bifunctional Porphyrin Anchor

  Vesicle Prep Pro publication in Angewandte Chemie International Edition (2013)

Authors: 
Burns J.R., Göpfrich K., Wood J.W.,Thacker V.V.,Stulz E., Keyser U.F., Howorka S.

2013 - Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures

  Vesicle Prep Pro publication in Chemistry and Physics of Lipids (2013)

Authors: 
Ostroumova O.S., Chulkov E.G., Stepanenko O.V., Schagina L.V.

2012 - Natural and artificial ion channels for biosensing platforms

   Port-a-Patch,      Patchliner,      SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and      Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

Authors: 
Steller L., Kreir M., Salzer R.

2012 - Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution

   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2012)

Authors: 
Birkner JP, Poolman B, Koçer A.

2012 - Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayer

   Port-a-Patch and      Vesicle Prep Pro publication in The Journal of Biological Chemistry (2012)

Authors: 
Carnarius C., Kreir M., Krick M., Methfessel C., Moehrle V., Valerius O., Brüggemann A., Steinem C., Fertig N.

2012 - Design, synthesis and functional analysis of dansylated polytheonamide mimic: an artificial Peptide ion channel

   Port-a-Patch and      Vesicle Prep Pro publication in Journal of the American Chemical Society (2012)

Authors: 
Itoh H., Matsuoka S., Kreir M., Inoue M.

2011 - Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins

  Port-a-Patch and      Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States (2011)

Authors: 
Shaya D., Kreir M., Robbins R.A., Wong S., Hammon J., Brüggemann A., Minor Jr. D.L.

2010 - Studying mechanosensitive ion channels using liposomes

  Port-a-Patch and      Vesicle Prep Pro publication in Methods in Molecular Biology (2010)

Authors: 
Martinac B., Rohde P.R., Battle A. R., Petrov E., Pal P, Alexander Foo A.FW, Vásquez V., Huynh T. and Kloda A.

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

Authors: 
Mahendran K.R., Kreir M., Weingart H., Fertig N., Winterhalter M.

2009 - The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

  Port-a-Patch and      Vesicle Prep Pro publication in Journal of Structural Biology (2009)

Authors:
Gaßmann O., Kreir M., Ambrosi C., Pranskevich J., Oshima A, Röling C., Sosinsky G., Fertig N., Steinem C.

2009 - Amyloid-beta-Induced Ion Flux in Artificial Lipid Bilayers and Neuronal Cells: Resolving a Controversy

  Port-a-Patch and      Vesicle Prep Pro publication in Neurotoxicity Research (2009)

Authors: 
Capone R., Quiroz F.G., Prangkio P., Saluja I., Sauer A.M., Bautista M.R., Turner R.S., Yang J., Mayer M.

2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes

  Port-a-Patch and      Vesicle Prep Pro publication in Lab-on-a-chip (2008)

Authors: 
Kreir M., Farre C., Beckler M., George M., Fertig N.

2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation

  Port-a-Patch and      Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)

Authors: 
Sondermann M., George M., Fertig N., Behrends J.C.