• Vesicle Prep Pro

    市场上第一款全自动制备无溶剂巨型单层囊泡(GUVs)的设备
  • Vesicle Prep Pro

    GUVs大小一致-适用与不同的应用

KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:  logo pdf   (0.2 MB)

Summary:

Solvent-free planar lipid bilayers were formed in an automated manner using suction to attract a giant unilamellar vesicle (GUV) to the patch clamp chip which subsequently bursts across the aperture. Incubation of GUV's with purified KcsA channel protein yielded proteoliposomes. These proteoliposomes allow for immediate recording of channel activity after GUV sealing. The rapid formation of protein-containing planar lipid bilayers is of potential use for the efficient electrophysiological characterization of KcsA as shown here and also other ion channel proteins of interest.

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