• Vesicle Prep Pro

    市场上第一款全自动制备无溶剂巨型单层囊泡(GUVs)的设备
  • Vesicle Prep Pro

    GUVs大小一致-适用与不同的应用

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

Authors: 
Mahendran K.R., Kreir M., Weingart H., Fertig N., Winterhalter M.

 

Journal: 
J. Biomol. Screen. (2010) 15(3):302-307


Abstract: 

A chip-based automated patch-clamp technique provides an attractive biophysical tool to quantify solute permeation through membrane channels. Proteo–giant unilamellar vesicles (proteo-GUVs) were used to form a stable lipid bilayer across a micrometer-sized hole. Because of the small size and hence low capacitance of the bilayer, single-channel recordings were achieved with very low background noise. The latter allowed the characterization of the influx of 2 major classes of antibiotics—cephalosporins and fluoroquinolones—through the major Escherichia coli porins OmpF and OmpC. Analyzing the ion current fluctuations in the presence of antibiotics revealed transport properties that allowed the authors to determine the mode of permeation. The chip-based setup allows rapid solution exchange and efficient quantification of antibiotic permeation through bacterial porins on a single-molecule level.


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