• Vesicle Prep Pro

    市场上第一款全自动制备无溶剂巨型单层囊泡(GUVs)的设备
  • Vesicle Prep Pro

    GUVs大小一致-适用与不同的应用

2021 - Alamethicin Channels as a Signal Transduction Element in an Immobilized Single Giant Unilamellar Vesicle

 icon vpp  Vesicle Prep Pro publication in Sensors and Materials (2021)

Authors:
Fukuda H., Nitta M., Sakamoto M., Shoji A., Sugawara M.

Journal:

Sensors and Materials (2021) doi: 10.18494/SAM.2021.3067


Abstract: 

A single giant unilamellar vesicle (GUV) functionalized with an anti-bovine serum albumin (BSA) antibody was immobilized on an avidin slip, and alamethicin channels were embedded as a signal transduction element for creating a channel-based molecular sensing system. The GUV sensor based on the membrane-bound anti-BSA antibody receptor exhibited alamethicin activities that reflected the binding of BSA (an analyte) at the membrane/solution interface. The normalized integrated current at −60 mV was able to be used as a measure of the amount of BSA in a solution. The quantification of BSA at pg/mL level was demonstrated.


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