Adenine Nucleotide Translocator (ANT) - Recorded on the Surfer

  SURFE²R N1 data and applications:

The Adenine Nucleotide Translocator (ANT) is located in the inner membrane of mitochondria and transports, as the name suggests, either ADP or ATP alone or against each other.  Recordings were done on mitochondria inner membrane preparations from pigs heart.  Example traces display the action of ANT either in the presence of ATP, in an other experiment in the presence of ADP.

AmtB - Activation by ammonium

 SURFE²R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE²R N1. Shown are transient currents measured after a 100 mM ammonium jump in empty liposomes (green) or proteoliposomes containing AmtB at a lipid protein ratio (LPR) of 50 (black), 10 (red) or 5 (blue). Insert: Normalized current measured in proteoliposomes containing AmtB at an LPR of 50 (black), 10 (red) or 5 (blue).

Data from Mirandela et al, 2018

AmtB - Substrate specificity

 SURFE²R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE²R N1. AmtB substrate specificity. (A) Transient current measured after a 100 mM substrate jump. Ammonium (red), methylammonium (black), potassium (green) or sodium (purple). Potassium and sodium do not act as substrates for AmtB and methylammonium translocates but at a much reduced rate compared with ammonium. Insert: Normalised current after a 100 mM substrate jump. Ammonium (red), methylammonium (black).

Data from Mirandela et al, 2018

ChR2 - Cell-based assay optimization

  SURFE²R N1 data and applications:

For assay optimization we checked the influence on cell suspension volume we have attached to one single sensor on the signal-to-noise ratio during the SURFE²R experiment.

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ChR2 - Exposure time and frequency of light

  SURFE²R N1 data and applications:

We investigated the influence of the duration and frequency of the light pulses for ChR2 activation on the stability of the ChR2 signals over time.

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ChR2 - Membrane potential as driving force

  SURFE²R N1 data and applications:

We investigated the effect of a membrane potential - generated by Na⁺/Ca²⁺ exchange activity of NCX1 - on the signal amplitude of light induced Na⁺ flux by ChR2.

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ChR2 - Stability of Retinal binding

  SURFE²R N1 data and applications:

We investigated the effects of retinal addition and removal on light-induced ChR2 activity using a prototypical SURFE²R N1 add-on for activation by light.

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ChR2 - Symmetry of transport

  SURFE²R N1 data and applications:

Depending on the direction of the Na⁺ gradient, we observed positive or negative transient currents after ChR2 activation by light. A negative control without ChR2 shows no current signal upon light activation.

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ChR2 - Variation of light intensity

  SURFE²R N1 data and applications:

We observed saturation kinetics for the light-induced ChR2 activity with increasing LED intensities using a prototypical SURFE²R N1 add-on for activation by light.

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ClC - EC50 for inward and outward directed chloride flux

  SURFE²R N1 data and applications:

We determined EC₅₀ and relative V_max for Cl^- in an Cl^-/H⁺ exchange assay for both transport directions and found a slight asymmetry in transporter kinetics.

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ClC - Inhibition with OADS

  SURFE²R N1 data and applications:

We investigated the effect of the specific ClC inhibitor OADS on the transport activity of ClC and its reversibility.

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ClC - Inward and outward directed chloride flux

  SURFE²R N1 data and applications:

According to the transport direction of 2Cl^-/1H⁺ exchange, we observed positive (Cl^- efflux) or negative (Cl^- influx) transient currents.

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ClC - Proteoliposomes with different lipid-to-protein ratios

  SURFE²R N1 data and applications:

We tested proteoliposomes with different densities of reconstituted ClC protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.

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EAAT3 - Current Traces at different Sodium Chloride Concentrations

   SURFE²R N1 data and applications:

EAAT3 current traces were recorded after application of different Sodium Chloride concentrations. The EC₅₀ of the peak current was determined as 42.2 mM. 

NCX_Mj - EC50 for Calcium and Magnesium

  SURFE²R N1 data and applications:

Measurement of the transport activity of NCX_Mj. The first solution exchange provides a Na⁺-free solution to establish an outward directed Na⁺ gradient; the second solution exchange provides the divalent cation to activate the ion exchanger. By applying different concentrations of one divalent cation on the same sensor, we determined EC₅₀ for Ca²⁺, Mg²⁺ and Sr²⁺.

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NCX1 - Block by KB-R7943

   SURFE²R N1 data and applications:

Cardiac NCX1 expressed in iCell^® Cardiomyocytes², Cor.4U^® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.   

NCX1 - Block by SEA0400

   SURFE²R N1 data and applications:

Cardiac NCX1 expressed in iCell^® Cardiomyocytes² or HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  

NCX1 - Current traces recorded from Cor.4U Cardiomyocytes, iCell Cardiomyocytes and HEK cells

   SURFE²R N1 data and applications:

Cardiac NCX1 was activated using 30 μM Ca²⁺ on the SURFE²R N1. NCX1 was recorded from Cor.4U^® Cardiomyocytes, iCell^® Cardiomyocytes² or overexpressed in HEK cells. The calcium affinity was determined for NCX1 recorded from iCell^® Cardiomyocytes² and HEK cells, shown on the right.  

Organic Cation Transporter 2 (OCT2) - Pharmacology

 SURFE²R N1 data and applications:

EC₅₀ values and relative V_max for three different substrates are shown.

The IC₅₀ value for Choline was determined as 4.6 mM, for TEA as 0.33 mM and for Metformin as 8.6 mM.

PepT1 - Pharmacology of Lys[Z(NO2)]-Val

  SURFE²R N1 data and applications:

Representive traces of PepT1 overexpressed in CHO cells. PepT1 (SLC15A1) is expressed mainly in intestine and kidney, it enables uptake of oligopeptides. The PepT1 signal is evoked by glycylglycin. The transport of oligopeptides can be inhibited by addition of Lys[Z(NO2)]-Val. The IC₅₀ of Lys[Z(NO2)]-Val was 380µM.

Respiratory Chain Complex I and III - Current Over Mitochondrial Membrane

  SURFE²R N1 data and applications:

In the respiratory chain Complex I and III, oxidation of NADH leads to transport of 4 protons across the membrane. The electrons are transferred to cytochrome c. Because of the electron leakage to oxygen, both complex are the main sites of production of harmful superoxide. In these experiments using the SURFE²R N1, application of 100µM NADH results in a current over the mitochondrial membrane, where Complex I and III are expressed.

Respiratory Chain Complex II and III - Current Traces

  SURFE²R N1 data and applications:

Respiratory Chain Complex II is a succinate dehydrogenase. In this complex, succinate is oxidated to fumarate. The electrons are transfered to the quinone pool, the Complex III (cytochrome bc₁ complex) and cytochrome c, while four protons are transported to the intermembrane space.

Respiratory Chain Complex IV - Current Traces

  SURFE²R N1 data and applications:

The Respiratory Chain Complex IV, known as Cytochrom c oxidase, reduces oxygen by cunsumption of electrons from cytocrome c and transport of two protons to the intermembrans space. Here the reaction runs backwards.

Respiratory Chain Complex V - Action of the ATP Synthase after Application of ATP

  SURFE²R N1 data and applications:

The Respiratory Chain Complex V uses the transmembrane proton gradient (produced by Complex I, III and IV) to generates ATP from ADP plus phosphate. One component of ATP synthase acts as an ion channel that provides for a proton flux back into the mitochondrial matrix. This reflux releases free energy, which is used to drive ATP synthesis, catalyzed by the other component of the complex. Experiments on the SURFE²R N1 show the action of the ATPase after application of ATP.

SERCA - IC50 for Cisplatin

  SURFE²R N1 data and applications:

Ca²⁺-transport in SERCA is triggered by ATP hydrolysis. Using ATP concentration jumps, the authors determined the IC₅₀ of SERCA inhibition by cisplatin

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SGLT1 - Current Traces after Application of alpha-MDG

   SURFE²R N1 data and applications:

Current traces after application of 10 mM and 50 mM alpha-MDG.

SGLT1 - EC50 for D-glucose transport

   SURFE²R N1 data and applications:

Short Description: Na+/sugar translocation is detected using a sugar concentration jump in presence of sodium. Here we determined the KM for glucose transport by SGLT1. 
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SGLT1 - Sugar transport and binding

   SURFE²R N1 data and applications:

Short Description: Na⁺/sugar translocation is detected using a sugar concentration jump in presence of sodium. In absence of sodium, we found a pre steady-state signal, representing sugar binding. Under these conditions, no transport is observed. 
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Sodium-Potassium ATPase - Analysis

  SURFE²R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na⁺/K⁺ pump is responsible for the active transport of Na⁺ and K⁺ in the cells containing relatively high concentrations of K⁺ ions but low concentrations of Na⁺ ions. The Na⁺/K⁺-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na⁺ and K⁺, or in the presence of ATP and Na⁺.

2022 - Structures and mechanism of the plant PIN-FORMED auxin transporter

    SURFE²R N1 publication in Nature (2022)

Authors:
Ung K.L., Winkler M., Schulz L., Kolb M., Janacek D.P., Dedic E., Stokes D.L., Hammes U.Z., Pedersen B.P.

2022 - Structure, mechanism and lipid-mediated remodeling of the mammalian Na+/H+ exchanger NHA2

    SURFE²R N1 publication in Nature Structural & Molecular Biology (2022)

Authors:
Matsuoka R., Fudim R., Jung S., Zhang C., Bazzone A., Chatzikyriakidou Y., Robinson C. V., Nomura N., Iwata S., Landreh M., Orellana L., Beckstein O., Drew D.

2022 - SSM-based electrophysiology, a label-free real-time method reveals sugar binding & transport events in SGLT1

    SURFE²R N1 publication in Biosensors and Bioelectronics (2022)

Authors:
Bazzone A., Körner A., Meincke M., Bhatt M., Srujan S., Barthmes M., Kubick S., Fertig N.

2022 - Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification

    SURFE²R N1 Publication in Science Advances (2022)

Authors:
Bärland N., Rueff A.S., Cebrero G., Hutter C.A.J., Seeger M.A., Veening J.W., Perez C.

2022 - Expression, purification and characterization of human proton-coupled oligopeptide transporter 1 hPEPT1

    SURFE²R N1 publication in Protein Expression and Purification (2022)

Authors:
Rafiq M., Ernst H.A., Aduri N.G., Prabhala B.K., Tufail S., Rahman M., Bloch M.B., Mirza N., Taylor N., Boesen T., Gajhede M., Mirzaa O.

2022 - Electrogenic reaction step and phospholipid translocation pathway of the mammalian P4-ATPase ATP8A2

    SURFE²R N1 publication in FEBS Letters (2022)

Authors:
Tadini-Buoninsegni F., Mikkelsen S.A., Mogensen L.S., Holm R., Molday R.S., Andersen J.P.

2022 - Crystal structures of bacterial Small Multidrug Resistance transporter EmrE in complex with structurally diverse substrates

 SURFE²R N1 Publication in eLife (2022)

Authors:
Kermani A.A., Burata O.E., Koff B.B., Koide A., Koide S., Stockbridge R.B.

2022 - Coexistence of Ammonium Transporter and Channel Mechanisms in Amt-Mep-Rh Twin-His Variants Impairs the Filamentation Signaling Capacity of Fungal Mep2 Transceptors

    SURFE²R N1 publication in mBio (2022)

Authors:
Williamson G., Brito A. S., Bizior A., Tamburrino G., Mirandela G. D., Harris T., Hoskisson P. A., Zachariae U., Marini A. M., Boeckstaens M., Javelle A.

2021 - The CopA2-Type P1B-Type ATPase CcoI Serves as Central Hub for cbb3-Type Cytochrome Oxidase Biogenesis

    SURFE²R N1 publication in Frontiers in Microbiology (2021)

Authors:
Andrei A., Di Renzo M.A., Öztürk Y., Meisner A., Daum N., Frank F., Rauch J., Daldal F., Andrade S.L.A., and Koch H-G.

2021 - Structure and lipid-mediated remodelling mechanism of the Na+/H+ exchanger NHA2

 SURFE²R N1 Publication in BioRxiv (2021)

Authors:
Matsuoka R., Fudim R., Jung S., Zhang C., Bazzone A., Chatzikyriakidou Y., Nomura N., Iwata S., Orellana L., Beckstein O., Drew D.

2021 - Structure and dynamics of the drug-bound bacterial transporter EmrE in lipid bilayers

    SURFE²R N1 publication in Nature Communications(2021)

Authors:
Shcherbakov A.A., Hisao G., Mandala V.S., Thomas N.E., Soltani M., Salter E.A., Davis Jr J.H., Henzler-Wildman K.A., Hong M.

2021 - Structural basis for Potassium transport by KdpFABC

   SURFE²R N1 pre-print in bioRxiv (2021)

Authors:

Sweet M.E., Larsen C., Zhang X., Schlame M., Pedersen B.P., Stokes D.L.

2021 - Stimulation of Ca2+‐ATPase Transport Activity by a Small‐Molecule Drug

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ChemMedChem (2021)

Authors:
Sordi G., Goti A., Young H.S., Palchetti I., Tadini‐Buoninsegni F.

2021 - Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology

    SURFE²R N1 publication in Journal of Visualized Experiments (styled JoVE) (2021)

Authors:
Bärland N., Perez C.

2021 - Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification

    SURFE²R N1 pre-print publication in bioRxiv (2021)

Authors:
Bärland N., Rueff A-S., Cebrero G., Hutter C. A. J., Seeger M. A., Veening J-W., Perez C.

2021 - Functional Characterization of SLC Transporters Using Solid Supported Membranes

SURFE²R N1 Chapter in Biophysics of Membrane Proteins (2021)

Authors:
Bazzone A., Barthmes M.

2021 - Engineering and functional characterization of a proton-driven β-lactam antibiotic translocation module for bionanotechnological applications

    SURFE²R N1 publication in Nature Scientific Reports (2021)

Authors:
Stauffer M., Ucurum Z., Harder D., Fotiadis D.

2021 - Cryo-EM structures of excitatory amino acid transporter 3 visualize coupled substrate, sodium, and proton binding and transport

 SURFE²R N1 publication in Science Advances (2021)

Authors:
Qiu B., Matthies D., Fortea E., Yu Z., Boudker O.

2021 - An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters

  SURFE²R N1 and     SURFE²R 96SE review publication in Frontiers in Pharmacology (2021)

Authors:
Dvorak V., Wiedmer T., Ingles-Prieto A., Altermatt P., Batoulis H., Bärenz F., Bender E., Digles D., Dürrenberger F., Heitman L.H., IJzerman A.P., Kell D.B., Kickinger S., Körzö D., Leippe P., Licher T., Manolova V., Rizzetto R., Sassone F., Scarabottolo L., Schlessinger A., Schneider V., Sijben H.J., Steck A-L., Sundström H., Tremolada S., Wilhelm M., Wright Muelas M., Zindel D., Steppan C-M., Superti-Furga G.

2021 - A Solid Supported Membrane-Based Technology for Electrophysical Screening of B0AT1-Modulating Compounds

   SURFE²R 96SE Publication in SLAS DISCOVERY: Advancing the Science of Drug Discovery. (2021)

Authors:
Gerbeth-Kreul C., Pommereau A., Ruf S., Kane Jr J.L., Kuntzweiler T., Hessler G., Engel C.K., Shum P., Wei L., Czech J., Licher T.

2021 - A Solid Supported Membrane Electrophysiology Assay for Efficient Characterization of Ion-Coupled Transport

    SURFE²R N1 publication in Journal of Biological Chemistry (2021)

Authors:
Thomas N.E., Feng W., Henzler-Wildman K.A.

2021 - A mechanistic switch from selective transporter to an ion channel impairs the filamentation signalling capability of ammonium transceptors in yeast

    SURFE²R N1 pre-print publication in bioRxiv (2021)

Authors:
Williamson G., Brito A. S., Bizior A., Tamburrino G., Mirandela G. D., Harris T., Hoskisson P. A., Zachariae U., Marini A. M., Boeckstaens M., Javelle A.

2020 - Unlocking the Reversal Potential of Solid Supported Membrane Electrophysiology to Determine Transport Stoichiometry

   SURFE²R N1 pre-publication in bioRxiv (2020)

Authors:
Henzler-Wildman K.A., Thomas N.E.

2020 - The structural basis of promiscuity in small multidrug resistance transporters

    SURFE²R N1 publication in Nature Communications (2020)

Authors:
Kermani A.A., Macdonald C.B., Burata O.E., Koff B.B., Koide A., Denbaum E., Koide S., Stockbridge R.B.

2020 - Protein Adsorption on Solid Supported Membranes: Monitoring the Transport Activity of P-Type ATPases

   SURFE²R 96SE and       SURFE²R N1 Review in Molecules (2020)

Authors:
Tadini-Buoninsegni F.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level (2)

  SURFE²R 96SE and       SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors: 
Tadini-Buoninsegni F.,Palchetti I.

2020 - Energy Coupling in Cation-Pumping Pyrophosphatase—Back to Mitchell

   SURFE²R N1 publication in Frontiers in Plant Science (2020)

Authors:
Baykov A.A.

2020 - Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis

 SURFE²R N1 publication in Membranes (2020)

Authors:
Gantner M., Laftsoglou T., Rong H., Postis V.L.G., Jueken L.J.C.

2020 - A two-lane mechanism for selective biological ammonium transport

   SURFE²R N1 publication in eLife (2020)

Authors:
Williamson G., Tamburrino G., Bizior A., Boeckstaens M., Mirandela G.D., Bage M.G., Pisliakov A., Ives C.M., Terras E., Hoskisson P.A., Marini A.M., Zachariae U., Javelle A.

2019 - Phosphatidylserine flipping by the P4-ATPase ATP8A2 is electrogenic

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PNAS (2019)

Authors:
Tadini-Buoninsegni F., Mikkelsen S.A., Mogensen L.S., Molday R.S., and Andersen J.P.

2019 - Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

   SURFE²R N1 publication in Nature Scientific Reports (2019)

Authors:
Patiño-Ruiz M., Fendler K., & Călinescu O.

2019 - Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

   SURFE²R N1 and      Orbit 16 publication in Frontiers in Pharmacology (2019)

Authors:
Dondapati S.K., Lübberding H., Zemella A., Thoring L., Wüstenhagen D.A., Kubick S.

2018 - The lipid environment determines the activity of the E. coli ammonium transporter, AmtB

   SURFE²R N1 publication in Faseb J. (2018)

Authors:
Mirandela G.D., Tamburrino G., Hoskisson P.A., Zachariae U., Javelle A.

2017 - SSM-Based Electrophysiology for Transporter Research

  SURFE²R N1 and     SURFE²R 96SE book chapter in Methody in Enzymology

Authors:
Bazzone A., Barthmes M., Fendler K.

2017 - Mechanisms of charge transfer in human copper ATPases ATP7A and ATP7B

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in IUBMB Life (2017)

Authors:
Tadini-Buoninsegni F., Smeazzetto S.

2017 - Insights into the mechanism of membrane pyrophosphatases by combining experiment and computer simulation

  SURFE²R N1 publication in Structural Dynamics (2017)

Authors: 
Shah N.R., Wilkinson C., Harborne S.P.D., Turku A., Li K.-M., Sun Y.-J., Harris S., Goldman A.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Discovery of Compounds that Positively Modulate the High Affinity Choline Transporter

  SURFE²R N1 publication in Frontiers in Molecular Neuroscience (2017)

Authors: 
Choudhary P., Armstrong E.J., Jorgensen C.C., Piotrowski M., Barthmes M., Torella R., Johnston S.E., Maruyama Y., Janiszewski J.S., Storer R.I., Skerratt S.E., Benn C.L.

2017 - Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2017)

Authors:
Smeazzetto S., Armanious G.P., Moncelli M.R., Bak J.J., Lemieux M.J., Young H.S., Tadini-Buoninsegni F.

2017 - Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

  SURFE²R-technology (custom-built system)  publication in PLoS ONE (2017)

Authors:
Patiño-Ruiz M., Ganea C., Fendler K., Călinescu O.

2016 - pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases

  SURFE²R-technology (custom-built system) publication in PLoS ONE (2016)

Authors:
Bazzone A., Madej M.G., Kaback H.R., Fendler K.

2016 - Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism

  SURFE²R N1 publication in Nature Communications (2016)

Authors: 
Li K., Wilkinson C., Kellosalo J., Tsai J., Kajander T, Jeuken L.J.C., Sun Y., Goldman A.

2016 - Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems

   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2016)

Authors:
Srinivasan L., Baars T.L., Fendler K., Michel H.

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

  SURFE²R N1 and      Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2016 - Electrogenic Cation Binding in the Electroneutral Na+/H+ Antiporter of Pyrococcus abyssi

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2016)

Authors:
Călinescu O., Linder M., Wöhlert D., Yildiz Ö., Kühlbrandt W., Fendler K.

2016 - Charge translocation by mitochondrial NADH:ubiquinone oxidoreductase (complex I) from Yarrowia lipolytica measured on solid-supported membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical and Biophysical Research Communications (2016)

Authors:
Siebels I., Dröse S.

2015 - Structural and Functional Studies of NirC from Salmonella typhimurium

   SURFE²R-technology (custom-built system) publication in Methods in Enzymology (2015)

Authors:
Rycovska-Blume A., Lü W., Andrade S., Fendler K., Einsle O.

2015 - Hofmeister effect of anions on calcium translocation by sarcoplasmic reticulum Ca2+-ATPase

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Nature Scientific Reports (2015)

Authors:
Tadini-Buoninsegni F., Moncelli M.R., Peruzzi N., Ninham B.W., Dei L., Lo Nostroa P.

2015 - A universal mechanism for transport and regulation of CPA sodium proton exchangers

  SURFE²R-technology (custom-built system) publication in Biological Chemistry (2015)

Authors:
Călinescu O., Fendler K.

2015 - A sulfur‐based transport pathway in Cu+‐ATPases

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in EMBO Reports (2015)

Authors:
Mattle D., Zhang L., Sitsel O., Pedersen L.T., Moncelli M.R., Tadini-Buoninsegni F., Gourdon P., Rees D.C., Nissen P., Meloni G.

2014 - Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling

  SURFE²R-technology (custom-built system) publication in Nature Communications (2014)

Authors:
Perez C., Faust B., Mehdipour A.R., Francesconi K.A., Forrest L.R., Ziegler C.

2014 - Species differences in bacterial NhaA Na+/H+ exchangers

  SURFE²R-technology (custom-built system) publication in FEBS Letters (2014)

Authors:
Călinescu O., Danner E., Böhm M., Hunte C., Fendler K..

2014 - NhaA Na+/H+ antiporter mutants that hardly react to the membrane potential

  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Alkoby D., Rimon A., Budak M., Patino-Ruiz M., Călinescu O., Fendler K., Padan E..

2014 - Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium

  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Lentes C.J., Mir S.H., Boehm M., Ganea C., Fendler K., Hunte C.

2014 - Keeping it simple, transport mechanism and pH regulation in Na+/H+ exchangers

   SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2014)

Authors:
Călinescu O., Paulino C., Kühlbrandt W., Fendler K.

2014 - Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch 96 Using Instant Frozen Cells

    SURFE²R N96 (predecessor model of SURFE²R 96SE) book chapter in Patch-Clamp Methods and Protocols

Authors:
Polonchuk L.

2014 - Direct observation of electrogenic NH4(+) transport in ammonium transport (Amt) proteins

   SURFE²R-technology (custom-built system) publication in PNAS (2014)

Authors:
Wacker T., Garcia-Celma J.J., Lewe P., Andrade S.L.

2014 - Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP-Dependent Ca2+ Translocation by Sarco-Endoplasmic Reticulum Ca2+-ATPase (SERCA)

  SURFE²R N1 publication in ChemMedChem (2014)

Authors: 
Sadafi F.Z., Massai L., Bartolommei G., Moncelli M.R., Messori L., Tadini-Buoninsegni F.

2013 - Photocycle and vectorial proton transfer in a rhodopsin from the eukaryote Oxyrrhis marina

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2013)

Authors:
Janke C., Scholz F., Becker-Baldus J., Glaubitz C., Wood P.G., Bamberg E., Wachtveitl J., Bamann C.

2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

   SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)

Authors:
Garcia-Celma J., Szydelko A., Dutzler R.

2013 - Enhanced adsorption of Ca-ATPase containing vesicles on a negatively charged solid supported membrane for the investigation of membrane transporters

  SURFE²R N1 publication in Langmuir (2013)

Authors: 
Sacconi A., Moncelli M.R., Mergheri G., Tadini-Buoninsegni F.

2013 - Electrophysiological characterization of uncoupled mutants of LacY

  SURFE²R-technology (custom-built system) publication in Biochemistry (2013)

Authors:
Gaiko O., Bazzone A., Fendler K., Kaback H.R.

2013 - Differential Effects of Mutations on the Transport Properties of the Na+/H+ Antiporter NhaA from Escherichia coli*

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2013)

Authors:
Mager T., Braner M., Kubsch B., Hatahet L., Alkoby D., Rimon A., Padan E., Fendler K.

2012 - The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter

   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2012)

Authors:
Rycovska A., Hatahet L., Fendler K., Michel H.

2012 - Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP

  SURFE²R-technology (custom-built system) publication in PNAS (2012)

Authors:
Khafizov K., Perez C., Koshy C., Quick M., Fendler K., Ziegler C., Forrest L.R.

2012 - Distinctive features of catalytic and transport mechanisms in mammalian sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) and Cu+ (ATP7A/B) ATPases

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2012)

Authors:
Lewis D., Pilankatta R., Inesi G., Bartolommei G., Moncelli M.R., Tadini-Buoninsegni F.

2012 - Assaying the proton transport and regulation of UCP1 using solid supported membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in European Biophysics Journal (2012)

Authors:
Blesneac I., Ravaud S., Machillot P., Zoonens M., Masscheylen S., Miroux B., Vivaudou M., Pebay-Peyroula E.

2011 - Transport Mechanism and pH Regulation of the Na+/H+ Antiporter NhaA from Escherichia coli

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2011)

Authors:
Mager T., Rimon A., Padan E., Fendler K.

2011 - Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

  SURFE²R ONE (a predecessor model of the SURFE²R N1) publication in Australian Journal of Chemistry (2011)

Authors:
Gaiko O., Janausch I., Geibel S., Vollert H., Arndt P., Gonski S., Fendler K.

2011 - G117C MelB, a mutant melibiose permease with a changed conformational equilibrium

  SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Ganea C., Meyer-Lipp K., Lemonnier R., Krah A., Leblanc G., Fendler K.

2011 - Development of an assay for Complex I/Complex III of the respiratory chain using solid supported membranes and its application in mitochondrial toxicity screening in drug discovery

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2011)

Authors:
Preissl S., Bick I., Obrdlik P., Diekert K., Gul S., Gribbon P.

2011 - Characterisation of the purified human sodium/iodide symporter reveals that the protein is mainly present in a dimeric form and permits the detailed study of a native C-terminal fragment

   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Huc-Brandt S., Marcellin D., Graslin F., Averseng O., Bellanger L., Hivin P., Quemeneur E., Basquin C., Navarro V., Pourcher T., Darrouzet E.

2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)

Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.

2010 - Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Pflügers Archiv - European Journal of Physiology (2010)

Authors:
Balannik V., Obrdlik P., Inayat S., Steensen C., Wang J., Rausch J.M., DeGrado W.F., Kelety B., Pinto L.H.

2010 - Electrophysiology of respiratory chain complexes and the ADP-ATP exchanger in native mitochondrial membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2010)

Authors:
Watzke N., Diekert K., Obrdlik P.

2010 - Electrophysiological characterization of ATPases in native synaptic vesicles and synaptic plasma membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical Journal (2010)

Authors:
Obrdlik P., Diekert K., Watzke N., Keipert C., Pehl U., Brosch C., Boehm N., Bick I., Ruitenberg M., Volknandt W., Kelety B.

2010 - Delineating electrogenic reactions during lactose/H+ symport

  SURFE²R-technology (custom-built system) publication in Biochemistry (2010)

Authors:
Garcia-Celma J.J., Ploch J., Smirnova I., Kaback H.R., Fendler K.

2010 - ATP dependent charge movement in ATP7B Cu+-ATPase is demonstrated by pre-steady state electrical measurements

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2010)

Authors:
Tadini-Buoninsegni F., Bartolommei G., Moncelli M.R., Pilankatta R., Lewis D., Inesi G.

2009 - Measuring Ion Channels on Solid Supported Membranes

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2009)

Authors:
Schulz P., Dueck B., Mourot A., Hatahet L., Fendler K.

2009 - Inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Chemical Research in Toxicology (2009)

Authors:
Gramigni E., Tadini-Buoninsegni F., Bartolommei G., Santini G., Chelazzi G., Moncelli M.R.

2009 - Electrophysiological characterization of LacY

  SURFE²R-technology (custom-built system) publication in PNAS (2009)

Authors:
Garcia-Celma J.J., Smirnova I.N., Kaback H.R., Fendler K.

2009 - Electrogenic ion pumps investigated on a solid supported membrane: comparison of current and voltage measurements

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Langmuir (2009)

Authors:
Bartolommei G., Moncelli M.R., Rispoli G., Kelety B., Tadini-Buoninsegni F.

2009 - DtpB (YhiP) and DtpA (TppB, YdgR) are prototypical proton-dependent peptide transporters of Escherichia coli

  SURFE²R ONE (a predecessor model of SURFE²R N1) publication in The FEBS Journal (2009)

Authors:
Harder D., Stolz J., Casagrande F., Obrdlik P., Weitz D., Fotiadis D., Daniel H.

2009 - An automatic electrophysiological assay for the neuronal glutamate transporter mEAAC1

   SURFE²R-technology (custom-built system) publication in Journal of Neuroscience Methods (2009)

Authors:
Krause R., Watzke N., Kelety B., Dörner W., Fendler K.

2008 - Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane

  SURFE²R-technology (custom-built system) publication in Langmuir (2008)

Authors:
Garcia-Celma J.J., Dueck B., Stein M., Schlueter M., Meyer-Lipp K., Leblanc G., Fendler K.

2008 - Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2008)

Authors:
Wimmer F., Oberwinkler T., Bisle B., Tittor J., Oesterhelt D.

2008 - Effect of Clotrimazole on the Pump Cycle of the Na,K-ATPase

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2008)

Authors:
Bartolommei G., Devaux N., Tadini-Buoninsegni F., Moncelli M., Apell H.-J.

2007 - Functional and structural characterization of a prokaryotic peptide transporter with features similar to mammalian PEPT1

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2007)

Authors:
Weitz D., Harder D., Casagrande F., Fotiadis D., Obrdlik P., Kelety B., Daniel H.

2006 - Transporter assays using solid supported membranes: a novel screening platform for drug discovery

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2006)

Authors:
Kelety B., Diekert K., Tobien J., Watzke N., Dörner W., Obrdlik P., Fendler K.

2006 - The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding

  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2006)

Authors:
Meyer-Lipp K., Séry N., Ganea C., Basquin C., Fendler K., Leblanc G.

2006 - Structure and function of prokaryotic glutamate transporters from Escherichia coli and Pyrococcus horikoshii

   SURFE²R-technology (custom-built system) publication in Biochemistry (2006)

Authors:
Raunser S., Appel M., Ganea C., Geldmacher-Kaufer U., Fendler K., Kühlbrandt W.

2006 - Establishment of Cell-Free Electrophysiology for Ion Transporters: Application for Pharmacological Profiling

   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biomolecular Screening (2006)

Authors:
Geibel S., Flores-Herr N., Licher T., Vollert H.

2005 - Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli

   SURFE²R 500 (a predecessor model of SURFE²R N1) publication in Biochimica et Biophysica Acta (BBA) - Bioenergetics (2005)

Authors:
Zuber D., Krause R., Venturi M., Padan E., Bamberg E., Fendler K.

2004 - Time-resolved charge translocation by sarcoplasmic reticulum Ca-ATPase measured on a solid supported membrane

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2004)

Authors:
Tadini Buoninsegni F., Bartolommei G., Moncelli M.R., Inesi G., Guidelli R.

2004 - Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli

   SURFE²R-technology (custom-built system) publication in Journal of Molecular Biology (2004)

Authors:
Zhou A., Wozniak A., Meyer-Lipp K., Nietschke M., Jung H., Fendler K.

2004 - Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli

   SURFE²R-technology (custom-built system) publication in Journal of Molecular Biology (2004)

Authors:
Zhou A., Wozniak A., Meyer-Lipp K., Nietschke M., Jung H., Fendler K.

2004 - Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane

   SURFE²R-technology (custom-built system) publication in Bioelectrochemistry (2004)

Authors:
Bartolommei G., Buoninsegni F.T., Moncelli M.R.

2003 - Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2003)

Authors:
Burzik C., Kaim G., Dimroth P., Bamberg E., Fendler K.

2002 - Photocurrents Generated by Bacteriorhodopsin Adsorbed on Thiol/Lipid Bilayers Supported by Mercury

   SURFE²R-technology (custom-built system) publication in Langmuir (2002)

Authors:
Dolfi A., Tadini-Buoninsegni F., Moncelli M.R., Guidelli R.

2001 - Evidence for intraprotein charge transfer during the transport activity of the melibiose permease from Escherichia coli

  SURFE²R-technology (custom-built system) publication in Biochemistry (2001)

Authors:
Ganea C., Pourcher T., Leblanc G., Fendler K.

1999 - Kinetics of electrogenic transport by the ADP/ATP carrier

  SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Gropp T., Brustovetsky N., Klingenberg M., Müller V., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na/K-ATPase Investigated on Solid Supported Membranes: Rapid Solution Exchange with a New Technique

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K.

1993 - Charge transport by ion translocating membrane proteins on solid supported membranes

   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1993)

Authors:
Seifert K., Fendler K., Bamberg E.